Summary of Study ST002966

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001845. The data can be accessed directly via it's Project DOI: 10.21228/M87B0H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002966
Study Title	Metabolomics reveal the pathway of benzylisoquinoline alkaloids in Corydalis yanhusuo bulbs
Study Type	spatial and temporal distribution
Study Summary	In general, bulbs of Corydalis yanhusuo can be divided into "mother-bulb (MB)" and "son-bulb (SB)" according to different parts. The mother bulbs are formed by the degeneration and re-expansion of their original stem and are used as medicinal material in production. Son bulbs emerge from axillary buds on horizontally elongated rhizomes, of which the larger bulb can also be used as medicine, while the smaller bulb is reserved as a seed stem for "seed". In this study, materials of C. yanhusuo bulbs were cultivated in the field, which was proposed and identified by Professor Da-xia Chen. Widely targeted metabolome sequencing of C. yanhusuo bulbs was performed by UPLC-ESI MS/MS system, and its metabolites were successfully identified and annotated in self-built database (the MetWare database). A total of 702 metabolites were identified in all samples, including 216 alkaloids, 120 lipids, 67 amino acids and their derivatives, 59 organic acids, 63 phenolic acids, 19 terpenoids, 28 flavonoids, 4 lignin and coumarins, 43 nucleotides and their derivatives, 1 tannin, 3 quinones and 79 other substances. The numbers of up-accumulated and down-accumulated metabolites in MB-A vs MB-C and SB-A vs SB-C were 135 and 148, 90 and 210, respectively. There were 184 kinds of DAMs between SB-A and MB-A (including 144 down-accumulated and 40 up-accumulated compounds in the MB-A samples) and 127 kinds of DAMs between SB-C and MB-C (including 57 down-accumulated and 40 up-accumulated compounds in the MB-C samples) .
Institute	Chongqing Academy of Chinese Materia Medica, Chongqing, China
Laboratory	Department of Traditional Chinese Medicine
Last Name	Zhao
First Name	Xiao
Address	Nanshan stree, Chongqing, Nanan District, 400065, China
Email	321427953@qq.com
Phone	02389029062
Submit Date	2023-11-05
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	APCIESI
Release Date	2023-11-28
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003085
Sampleprep Summary:	After vacuum freeze-drying, the biological sample was crushed at 30 Hz for 1.5 minutes using a mixer (MM 400, Retsch) with zirconia beads. One hundred milligrams of lyophilized powder was accurately weighed, dissolved in 1.2 ml of 70% methanol solution, rotated every 30 minutes for 30 seconds, repeated 6 times, and placed in a refrigerator at 4 °C for 12 h. Finally, after centrifuging the solution at 12 000 rpm for 10 minutes, the extract was filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China, http://www.anpel.com.cn/) to perform UPLC‒MS/MS analysis.

Sampleprep ID:

SP003085

Sampleprep Summary:

After vacuum freeze-drying, the biological sample was crushed at 30 Hz for 1.5 minutes using a mixer (MM 400, Retsch) with zirconia beads. One hundred milligrams of lyophilized powder was accurately weighed, dissolved in 1.2 ml of 70% methanol solution, rotated every 30 minutes for 30 seconds, repeated 6 times, and placed in a refrigerator at 4 °C for 12 h. Finally, after centrifuging the solution at 12 000 rpm for 10 minutes, the extract was filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China, http://www.anpel.com.cn/) to perform UPLC‒MS/MS analysis.