Summary of Study ST003005
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001873. The data can be accessed directly via it's Project DOI: 10.21228/M8MF0Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003005 |
| Study Title | Brown fat metabolomics of Chow and HFD diet |
| Study Summary | Brown adipose tissue (BAT) metabolites in mice fed a standard diet or a high-fat diet for 12 weeks. N = 6 per group. |
| Institute | Harvard Medical School |
| Last Name | Wang |
| First Name | Dandan |
| Address | 3 Blackfan Circle, Boston, MA, 02115, USA |
| dandanwang2022@gmail.com | |
| Phone | 5083733714 |
| Submit Date | 2023-12-12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-02-19 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003125 |
| Sampleprep Summary: | Animals were sacrificed immediately by cervical dislocation and tissues were rapidly extracted and immediately snap frozen in liquid nitrogen for metabolite profiling. For metabolite extraction, tissues were weighed and then homogenized with extraction buffer which consisted of 80% methanol containing D₈-Phenylalanine internal standards at a 40:1 volume to wet weight ratio. Samples were centrifuged at 16,000 x g at 4 °C for 15 min. Finally, 50 μL supernatant was transferred to the glass insert for LC-MS detection. |
