Summary of Study ST003043

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001894. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ6G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003043
Study Title	Retinoic acid receptor alpha activity in proximal tubules prevents kidney injury and fibrosis
Study Summary	Retinoid levels of all-trans-retinol, retinoic acid, and retinyl palmitate were measured in the kidney and serum of GCERRARaD (kidney proximal tubule RARalpha knockout mice) females 3 days or 3 months post-tamoxifen (n=5/group) and age-matched Wild Type females (n=4).
Institute	Weill Cornell Medicine
Last Name	Tang
First Name	Xiao-Han
Address	1300 York Ave, New York, NY10065
Email	xit2001@med.cornell.edu
Phone	3478327329
Submit Date	2024-01-14
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2024-01-18
Release Version	1

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP003164
Sampleprep Summary:	To extract metabolites, frozen kidney cortices were weighed, and ca 20 mg of each cortex was added to a tube containing 650 μL of 80% methanol (diluted in dH2O). Sample tubes were placed in a bead homogenizer for 1 minute at a frequency of 30/s. Samples were then placed in a -20℃ freezer for 10 minutes, centrifuged for 10 minutes at 15,000 RPM in 4℃, and the supernatant was moved to a new tube. The remaining pellets were then re-extracted with 400 μL of 80% methanol, following the above procedure of homogenization and centrifugation. The supernatant was then added to the supernatant pool obtained from the first extraction. The pellet was re-extracted for a third time using 350 μL of 80% MeOH and supernatant was again added to the pool from the first two extractions. The supernatant pool was placed at -20℃ for 15 minutes, centrifuged for 20 minutes at 15,000 RPM, and transferred to a new tube. The methanolic extract samples were speed-vacuumed and reconstituted in 70% acetonitrile (diluted in dH2O) containing 0.025% acetic acid at a protein concentration of 3μg/μL. For serum preparation, samples were diluted 10-fold with 70% acetonitrile (diluted in dH2O) containing 0.025% acetic acid, and centrifuged for 20 minutes at 20,000 g.

Sampleprep ID:

SP003164

Sampleprep Summary:

To extract metabolites, frozen kidney cortices were weighed, and ca 20 mg of each cortex was added to a tube containing 650 μL of 80% methanol (diluted in dH2O). Sample tubes were placed in a bead homogenizer for 1 minute at a frequency of 30/s. Samples were then placed in a -20℃ freezer for 10 minutes, centrifuged for 10 minutes at 15,000 RPM in 4℃, and the supernatant was moved to a new tube. The remaining pellets were then re-extracted with 400 μL of 80% methanol, following the above procedure of homogenization and centrifugation. The supernatant was then added to the supernatant pool obtained from the first extraction. The pellet was re-extracted for a third time using 350 μL of 80% MeOH and supernatant was again added to the pool from the first two extractions. The supernatant pool was placed at -20℃ for 15 minutes, centrifuged for 20 minutes at 15,000 RPM, and transferred to a new tube. The methanolic extract samples were speed-vacuumed and reconstituted in 70% acetonitrile (diluted in dH2O) containing 0.025% acetic acid at a protein concentration of 3μg/μL. For serum preparation, samples were diluted 10-fold with 70% acetonitrile (diluted in dH2O) containing 0.025% acetic acid, and centrifuged for 20 minutes at 20,000 g.