Summary of Study ST003100
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003100 |
| Study Title | Lactiplantibacillus plantarum intervention on fecal bile acids of gestational fecal microbiome transplant germ-free mice |
| Study Summary | The study aims to investigate whether administration of probiotic L. plantarum could influence profiling of fecal bile acids of germ-free mice. L. plantarum during gestation of humanized GF mice showed that α-MCA, UCA, ACA, β-UDCA, and isoLCA were significantly increased. Other bile acids showed no significant difference. |
| Institute | Beijing Sanyuan Foods Co. Ltd |
| Last Name | Lijun |
| First Name | Chen |
| Address | 8th yingchang street, yinghai district |
| chenlijun@sanyuan.com.cn | |
| Phone | 01056306594 |
| Submit Date | 2024-02-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-08 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003221 |
| Sampleprep Summary: | Sample was extracted in 400 μL of methanol, vortexing and shaking for 60 s, add 100 mg of glass beads, and put into a high-throughput tissue grinder, grinding at 55 Hz for 60 s. Repeat the above operation at least twice; ultrasonic at room temperature for 30 min, centrifuged at 12,000 rpm at 4 ℃ for 10 min, and 200 μL of supernatant mixed with 400 μL of water. Take 200 μL of supernatant and add 400 μL of water, vortex for 30 s; take 300 μL of supernatant and filter it through 0.22 μm membrane, vortex for 30 s. After centrifugation, take 20 μL of supernatant and dilute it 10 times with 180 μL of 30% methanol solution, and add it to the assay vial. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
