Summary of Study ST001019

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000682. The data can be accessed directly via it's Project DOI: 10.21228/M8HQ2J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001019
Study Title	Lipidomic profiling of heart and plasma of mice following swim training versus pressure overload
Study Summary	Lipid profiling was performed on hearts and plasma from mice subjected to a physiological stimulus (4 weeks of swim exercise training) or pathological stimulus (4 weeks of pressure overload – transverse aortic constriction; TAC)
Institute	Baker Heart and Diabetes Institute
Laboratory	Cardiac Hypertrophy
Last Name	Tham
First Name	Yow Keat
Address	75 Commercial Rd, Melbourne 3004
Email	yowkeat.tham@baker.edu.au
Phone	+65385321266
Submit Date	2018-07-15
Raw Data File Type(s)	d
Analysis Type Detail	LC-MS
Release Date	2018-07-25
Release Version	1

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Treatment:

Treatment ID:	TR001072
Treatment Summary:	Swim training - Physiological hypertrophy in mice was achieved via swim training twice daily for 4 weeks, as previously described (McMullen et al., 2003). Swim training commenced in adult mice at approximately 3 months of age with 10 min sessions on the first day, with 10 min increments each day until the maximum of 90 min per session was reached. Mice were rested for at least 4 hours between each session, and water temperature was maintained between 30 to 32°C to avoid thermal stress. At the end of every session, mice were individually towel dried to prevent hypothermia. Plasma and hearts were collected 3 h after the last swim session. Transverse aortic constriction - Male mice were subjected to either sham or TAC surgery at approximately 3-3.5 months of age, as previously described (Xu et al., 2008). Two variations of TAC were performed, designated TAC-moderate (TAC-MOD) and TAC-severe (TAC-SEV). Aortic banding performed on the TAC-MOD mice used a blunt 25 G needle (outside diameter 0.5 mm) placed next to the aorta during ligation to obtain a consistent degree of banding (constriction of blood vessel from ~1 mm to 0.5 mm), while a 27 G needle (outside diameter 0.4 mm) was used for the TAC-SEV group (constriction of blood vessel from ~1 mm to 0.4 mm). Sham operated mice were subjected to the same procedure but no ligation was made.

Treatment ID:

TR001072

Treatment Summary:

Swim training - Physiological hypertrophy in mice was achieved via swim training twice daily for 4 weeks, as previously described (McMullen et al., 2003). Swim training commenced in adult mice at approximately 3 months of age with 10 min sessions on the first day, with 10 min increments each day until the maximum of 90 min per session was reached. Mice were rested for at least 4 hours between each session, and water temperature was maintained between 30 to 32°C to avoid thermal stress. At the end of every session, mice were individually towel dried to prevent hypothermia. Plasma and hearts were collected 3 h after the last swim session. Transverse aortic constriction - Male mice were subjected to either sham or TAC surgery at approximately 3-3.5 months of age, as previously described (Xu et al., 2008). Two variations of TAC were performed, designated TAC-moderate (TAC-MOD) and TAC-severe (TAC-SEV). Aortic banding performed on the TAC-MOD mice used a blunt 25 G needle (outside diameter 0.5 mm) placed next to the aorta during ligation to obtain a consistent degree of banding (constriction of blood vessel from ~1 mm to 0.5 mm), while a 27 G needle (outside diameter 0.4 mm) was used for the TAC-SEV group (constriction of blood vessel from ~1 mm to 0.4 mm). Sham operated mice were subjected to the same procedure but no ligation was made.