Summary of Study ST001223
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000820. The data can be accessed directly via it's Project DOI: 10.21228/M8PQ4V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001223 |
| Study Title | Host Metabolic Response in Early Lyme Disease |
| Study Summary | Lyme disease is a tick-borne bacterial illness that occurs in areas of North America, Europe, and Asia. Early infection typically presents as generalized symptoms with an erythema migrans (EM) skin lesion. Bacterial dissemination can result in multiple EM skin lesions or in extracutaneous manifestations such as Lyme neuroborreliosis. Metabolic biosignatures of patients with early Lyme disease can potentially provide diagnostic targets, as well as highlight metabolic pathways that contribute to pathogenesis. Sera from well-characterized patients diagnosed with either early localized Lyme disease (ELL) or early disseminated Lyme disease (EDL), plus healthy individuals (HC), from the United States were analyzed by liquid chromatography-mass spectrometry (LC-MS). Comparative analyses were performed between ELL, or EDL, or ELL combined with EDL, and the HC to develop biosignatures present in early Lyme disease. A direct comparison between ELL and EDL was also performed to develop a biosignature for stages of early Lyme disease. Metabolic pathway analysis and chemical identification of metabolites with LC-tandem mass spectrometry (LC-MS/MS) demonstrated alterations of eicosanoid, bile acid, sphingolipid, glycerophospholipid, and acylcarnitine metabolic pathways during early Lyme disease . These metabolic alterations were confirmed using a separate set of serum samples for validation. The findings demonstrated the metabolic pathways altered in the host during early Lyme disease and provide evidence that the diversity in the type of early Lyme disease manifestations may be associated with particular metabolic alterations. |
| Institute | Colorado State University |
| Department | Department of Microbiology, Immunology, and Pathology |
| Laboratory | Belisle |
| Last Name | Belisle |
| First Name | John |
| Address | 200 West Lake, Campus Delivery 0922, Colorado State University, Fort Collins, CO, 80523 |
| john.belisle@colostate.edu | |
| Phone | 9704915384 |
| Submit Date | 2019-07-02 |
| Publications | Host Metabolic Response in Early Lyme Disease Bryna L. Fitzgerald, Claudia R. Molins, M. Nurul Islam, Barbara Graham, Petronella R. Hove, Gary P. Wormser, Linden Hu, Laura V. Ashton, and John T. Belisle Journal of Proteome Research 2020 19 (2), 610-623 DOI: 10.1021/acs.jproteome.9b00470 |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-09-23 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR001305 |
| Treatment Summary: | N/A |
