Summary of Study ST001296

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000877. The data can be accessed directly via it's Project DOI: 10.21228/M8B382 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001296
Study Title	Metabolomics and Hormonomics to Crack the Code of Filbert Growth
Study Summary	Introduction: Plants respond to changes in their environments through hormonal activation of a physiological cascade that redirects metabolic resources and growth. In filberts (Corylus sp.), chelated iron promotes the growth of new shoots but the mechanism(s) are not understood. Objectives: To use untargeted metabolomics and hormonomics approaches to generate novel hypotheses for the morphoregulatory role of ferric ethylenediamine-N,N'-di-(ortho-hydroxyphenyl) acetic acid (Fe-EDDHA) in filbert shoot organogenesis in vitro. Methods: Data were generated using previously optimized standardized untargeted metabolomics protocols with time of flight mass spectrometry. Multivariate statistical tools (principal component and partial least squares discriminant analysis) did not detect significant differences. Discovery tools Significance Analysis of Microarrays (SAM), multiple linear regression analysis, Bayesian analysis, logical algorithms, machine learning, synthetic biotransformations, targeted hormonomics, and online resources including MetaboAnalyst were used. Results: Starch/sucrose metabolism and shikimate pathway metabolites were increased. Dose dependent decreases were found in polyphenol metabolism, specifically ellagic acid and its methylated derivative 3,4,3'-tri-O-methylellagic acid. Hormonomics analysis revealed significant differences in phytohormones and their conjugates. FeEDDHA treatment reduced indole-3-acetic acid, abscisic acid, salicylic acid, jasmonic acid conjugates (JA-Trp, JA-Ile, OH-JA) and dihydrozeatinglucoside in regenerating explants. Serotonin (5HT) was decreased in FeEDDHA-treated regenerating tissues while the related metabolite melatonin was increased. Eight phenolic conjugates of 5HT and eight catabolites were affected by FeEDDHA indicating that metabolism to sequester, deactivate and metabolize 5HT was induced by Fe(III). Tryptophan was metabolized through kynurenine but not anthranilate. Conclusion: Seven novel hypotheses were generated to guide future studies to understand the regulatory control(s) of shoot organogenesis.
Institute	University of British Columbia
Department	Chemistry
Laboratory	PlantSMART
Last Name	Murch
First Name	Susan
Address	3247 University Way
Email	susan.murch@ubc.ca
Phone	250-807-9566
Submit Date	2019-12-20
Raw Data Available	Yes
Raw Data File Type(s)	raw(Waters)
Analysis Type Detail	LC-MS
Release Date	2020-06-20
Release Version	1

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Treatment:

Treatment ID:	TR001385
Treatment Summary:	Filbert (C. avellana L. × C. americana Marshall cv. Geneva; Grimo Nut Nursery, Niagara-on-the-Lake, ON, Canada) shoot cultures were provided from germplasm maintained at the Gosling Research Institute for Plant Preservation (GRIPP; University of Guelph, Guelph, ON). Plantlets were grown in GA-7 vessels according to methods previously described (Garrison et al. 2013; Latawa et al. 2016). In brief, cultures were grown on semi-solid modified NCGR-COR medium (Yu and Reed 1995) supplemented with 10 g L-1 myo-inositol, 200 mg L-1 glycine, 100 mg L-1 nicotinic acid, 100 mg L-1 thiamine (PhytoTechnology Laboratories), 17.6 µM benzylaminopurine (BAP; Sigma-Aldrich), 0.014 µM indole-3-butyric acid (IBA; Sigma-Aldrich), 0.29 µM gibberellic acid (GA3; PhytoTechnology Laboratories), and 30 g L-1 glucose with 0, 230, 460 and 690 µM Fe-EDDHA (Sigma, St. Louis, MO) and the pH of the medium was adjusted to 5.7 before autoclaving at 121 ºC for 20 min. Cultures were maintained in a growth room at 23 ± 2oC with a 16 h photoperiod of 40 µmol m-2 s-1 provided by cool-white fluorescent lamps (Osram Sylvania Ltd., Mississauga, ON, Canada).

Treatment ID:

TR001385

Treatment Summary:

Filbert (C. avellana L. × C. americana Marshall cv. Geneva; Grimo Nut Nursery, Niagara-on-the-Lake, ON, Canada) shoot cultures were provided from germplasm maintained at the Gosling Research Institute for Plant Preservation (GRIPP; University of Guelph, Guelph, ON). Plantlets were grown in GA-7 vessels according to methods previously described (Garrison et al. 2013; Latawa et al. 2016). In brief, cultures were grown on semi-solid modified NCGR-COR medium (Yu and Reed 1995) supplemented with 10 g L-1 myo-inositol, 200 mg L-1 glycine, 100 mg L-1 nicotinic acid, 100 mg L-1 thiamine (PhytoTechnology Laboratories), 17.6 µM benzylaminopurine (BAP; Sigma-Aldrich), 0.014 µM indole-3-butyric acid (IBA; Sigma-Aldrich), 0.29 µM gibberellic acid (GA3; PhytoTechnology Laboratories), and 30 g L-1 glucose with 0, 230, 460 and 690 µM Fe-EDDHA (Sigma, St. Louis, MO) and the pH of the medium was adjusted to 5.7 before autoclaving at 121 ºC for 20 min. Cultures were maintained in a growth room at 23 ± 2oC with a 16 h photoperiod of 40 µmol m-2 s-1 provided by cool-white fluorescent lamps (Osram Sylvania Ltd., Mississauga, ON, Canada).