Summary of Study ST002057
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001302. The data can be accessed directly via it's Project DOI: 10.21228/M8DQ4C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002057 |
| Study Title | Distinct Human Hepatocyte Lipidomics Profiles for Nonalcoholic Steatohepatitis and In Vitro-Induced Steatosis |
| Study Summary | Nonalcoholic steatohepatitis (NASH) is a severe form of steatotic liver injury that can be caused by a variety of stimuli and has a significant mortality rate. A common technique to induce in vitro steatosis involves culturing primary human hepatocytes (PHH) in a fatty acid-enriched media. This study compared the lipidome of PHH cultured in a fatty acid-enriched media to hepatocytes from patients with NASH and healthy controls to determine whether such culture techniques could generate a hepatocellular lipid profile similar to that observed in NASH patients. LC-MS lipidomics analysis of hepatocytes from patients with NASH revealed increases in the total cellular abundance of glycerolipids, phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols, phosphatidylinositols and phosphatidylserines compared to healthy control hepatocytes. PHH cultured in a fatty acid-enriched environment demonstrated an increase in total lipid abundance, however, changes were limited to glycerolipids; in contrast to NASH hepatocytes, increases in the abundance of phospholipids were not observed. |
| Institute | Monash Institute of Pharmaceutical Sciences |
| Last Name | Kralj |
| First Name | Thomas |
| Address | 381 Royal Parade |
| Tom.Kralj@monash.edu | |
| Phone | +61 3 9902 6000 |
| Submit Date | 2021-12-08 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-01-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR002151 |
| Treatment Summary: | NASH hepatocytes were taken from patients with NASH. IVIS Hepatocytes were cultured and treated with fatty acid enriched media |
| Treatment Compound: | Free fatty acids |
| Cell Growth Config: | 24-well plate |
