Summary of Study ST002330

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001494. The data can be accessed directly via it's Project DOI: 10.21228/M8MD91 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002330
Study TitleEarly-stage responses to Plasmodiophora brassicae at the metabolome levels in clubroot resistant and susceptible oilseed Brassica napus
Study TypeTimecourse experiment
Study SummaryA total of 36 samples comprised two types of genotypes [CR (5 individuals pooled in each biological replicate) and CS (5 individuals pooled in each biological replicate)], two treatments (inoculated and uninoculated) and three biological replicates generated from three independent experiments and collected at 1-, 4-, and 7–DPI were used to extract primary and secondary metabolites and analyse the differences among the treatments.
Institute
Trent University
DepartmentBiology
LaboratoryEmery Lab
Last NameKisiala
First NameAnna
Address1600 West Bank Drive, Trent University
Emailannakisiala@trentu.ca
Phone7057481011
Submit Date2022-10-17
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-11-16
Release Version1
Anna Kisiala Anna Kisiala
https://dx.doi.org/10.21228/M8MD91
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR002429
Treatment Summary:A single spore-derived isolate of P. brassicae pathotype 3H (P3H) was used in this study. To assess the disease progression post-inoculation, histology, and scanning electron microscopy (SEM) was conducted on P. brassicae inoculated and uninoculated root tissues. Samples were collected by Adhikary as follows: seedlings were randomly selected, gently uprooted from the soil, the roots were washed thoroughly and collected in triplicates at after 24 hours of inoculation [hereinafter indicated as 1-day post-inoculation (DPI)], 4-, and 7-DPI.
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