Summary of Study ST001115
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000746. The data can be accessed directly via it's Project DOI: 10.21228/M87X0C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001115 |
| Study Title | Growth cone-enriched lipidome of embryonic to early postnatal mouse brain |
| Study Summary | A growth cone (GC) is a part of a neuron considered a hub for axon growth, motility and guidance functions. Unraveling the molecular composition of GCs and events through which active GCs transition to terminal synapses is of importance to developmental and regenerative neuroscience research. Here, we present a dataset on the lipid profiling of the growth cone-enriched fractions derived from E18, P0, P3, P6 and P9 C57BL/6J mouse brains. For comparison, we analyzed non-growth cone membranes. |
| Institute | University of Miami |
| Department | Ophthalmology, Bascom Palmer Eye Institute |
| Laboratory | Sanjoy K. Bhattacharya |
| Last Name | Bhattacharya |
| First Name | Sanjoy |
| Address | 1638 NW 10th Ave, Miami, FL 33136 |
| sbhattacharya@med.miami.edu | |
| Phone | +1 (305) 4824103 |
| Submit Date | 2018-11-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-01-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000746 |
| Project DOI: | doi: 10.21228/M87X0C |
| Project Title: | Growth cone-enriched lipidome of embryonic to early postnatal mouse brain |
| Project Summary: | A growth cone (GC) is a part of a neuron considered a hub for axon growth, motility and guidance functions. Unraveling the molecular composition of GCs and events through which active GCs transition to terminal synapses is of importance to developmental and regenerative neuroscience research. Here, we present a dataset on the lipid profiling of the growth cone-enriched fractions derived from E18, P0, P3, P6 and P9 C57BL/6J mouse brains. For comparison, we analyzed non-growth cone membranes. |
| Institute: | University of Miami |
| Department: | Ophthalmology, Bascom Palmer Eye Institute |
| Laboratory: | Sanjoy K. Bhattacharya |
| Last Name: | Bhattacharya |
| First Name: | Sanjoy |
| Address: | 1638 NW 10th Ave, Miami, FL 33136 |
| Email: | sbhattacharya@med.miami.edu |
| Phone: | +1 (305) 4824103 |
| Contributors: | Sanjoy K. Bhattacharya, Anna M. Trzeciecka |
Subject:
| Subject ID: | SU001161 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Animal Animal Supplier: | The Jackson Laboratory |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Fraction |
|---|---|---|
| SA076141 | GC_E18_1 | GC | Age:E18 |
| SA076142 | GC_E18_6 | GC | Age:E18 |
| SA076143 | GC_E18_5 | GC | Age:E18 |
| SA076144 | GC_E18_2 | GC | Age:E18 |
| SA076145 | GC_E18_3 | GC | Age:E18 |
| SA076146 | GC_E18_4 | GC | Age:E18 |
| SA076147 | GC_P0_6 | GC | Age:P0 |
| SA076148 | GC_P0_5 | GC | Age:P0 |
| SA076149 | GC_P0_2 | GC | Age:P0 |
| SA076150 | GC_P0_1 | GC | Age:P0 |
| SA076151 | GC_P0_4 | GC | Age:P0 |
| SA076152 | GC_P0_3 | GC | Age:P0 |
| SA076153 | GC_P3_6 | GC | Age:P3 |
| SA076154 | GC_P3_4 | GC | Age:P3 |
| SA076155 | GC_P3_5 | GC | Age:P3 |
| SA076156 | GC_P3_1 | GC | Age:P3 |
| SA076157 | GC_P3_2 | GC | Age:P3 |
| SA076158 | GC_P3_3 | GC | Age:P3 |
| SA076159 | GC_P6_6 | GC | Age:P6 |
| SA076160 | GC_P6_5 | GC | Age:P6 |
| SA076161 | GC_P6_4 | GC | Age:P6 |
| SA076162 | GC_P6_1 | GC | Age:P6 |
| SA076163 | GC_P6_2 | GC | Age:P6 |
| SA076164 | GC_P6_3 | GC | Age:P6 |
| SA076165 | GC_P9_6 | GC | Age:P9 |
| SA076166 | GC_P9_5 | GC | Age:P9 |
| SA076167 | GC_P9_1 | GC | Age:P9 |
| SA076168 | GC_P9_4 | GC | Age:P9 |
| SA076169 | GC_P9_2 | GC | Age:P9 |
| SA076170 | GC_P9_3 | GC | Age:P9 |
| SA076171 | M_E18_6 | M | Age:E18 |
| SA076172 | M_E18_5 | M | Age:E18 |
| SA076173 | M_E18_1 | M | Age:E18 |
| SA076174 | M_E18_2 | M | Age:E18 |
| SA076175 | M_E18_4 | M | Age:E18 |
| SA076176 | M_E18_3 | M | Age:E18 |
| SA076177 | M_P0_6 | M | Age:P0 |
| SA076178 | M_P0_5 | M | Age:P0 |
| SA076179 | M_P0_4 | M | Age:P0 |
| SA076180 | M_P0_1 | M | Age:P0 |
| SA076181 | M_P0_2 | M | Age:P0 |
| SA076182 | M_P0_3 | M | Age:P0 |
| SA076183 | M_P3_5 | M | Age:P3 |
| SA076184 | M_P3_4 | M | Age:P3 |
| SA076185 | M_P3_6 | M | Age:P3 |
| SA076186 | M_P3_3 | M | Age:P3 |
| SA076187 | M_P3_1 | M | Age:P3 |
| SA076188 | M_P3_2 | M | Age:P3 |
| SA076189 | M_P6_5 | M | Age:P6 |
| SA076190 | M_P6_4 | M | Age:P6 |
| SA076191 | M_P6_6 | M | Age:P6 |
| SA076192 | M_P6_3 | M | Age:P6 |
| SA076193 | M_P6_1 | M | Age:P6 |
| SA076194 | M_P6_2 | M | Age:P6 |
| SA076195 | M_P9_6 | M | Age:P9 |
| SA076196 | M_P9_5 | M | Age:P9 |
| SA076197 | M_P9_3 | M | Age:P9 |
| SA076198 | M_P9_1 | M | Age:P9 |
| SA076199 | M_P9_2 | M | Age:P9 |
| SA076200 | M_P9_4 | M | Age:P9 |
| Showing results 1 to 60 of 60 |
Collection:
| Collection ID: | CO001155 |
| Collection Summary: | Growth cones (GC) were enriched by sucrose-gradient centrifugation from embryonic and postnatal C57BL/6J mouse brains. Non-GC membranes (M) were collected for comparison. |
| Sample Type: | Brain |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001175 |
| Treatment Summary: | As described in the method. |
Sample Preparation:
| Sampleprep ID: | SP001168 |
| Sampleprep Summary: | As described in the method. |
| Extract Storage: | -20℃ |
Combined analysis:
| Analysis ID | AN001811 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Accela 600 |
| Column | Thermo Acclaim C30 (150 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | main area |
Chromatography:
| Chromatography ID: | CH001278 |
| Chromatography Summary: | Reversed phase chromatographic separation utilized Accela Autosampler (Thermo), Accela 600 pump (Thermo) and Acclaim C30 column: 3 µm, 2.1x150 mm (Thermo). The column temperature was maintained at 30˚C and tray temperature at 20˚C. Solvent A was composed of 10 mM ammonium acetate (LC-MS grade) in 60:40 methanol:water (LC-MS grade) with 0.2% formic acid (FA; LC-MS grade). Solvent B was composed of 10 mM ammonium acetate with 60:40 methanol:chloroform with 0.2% FA. The flow rate was 260 µL/min and injection volume was 10 µL. The gradient was 35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min. |
| Instrument Name: | Thermo Accela 600 |
| Column Name: | Thermo Acclaim C30 (150 x 2.1mm,3um) |
| Column Temperature: | 30 |
| Flow Gradient: | 35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min |
| Flow Rate: | 260 µL/min |
| Solvent A: | 60% methanol/40% water; 0.2% formic acid; 10 mM ammonium acetate |
| Solvent B: | 60% methanol/40% chloroform; 0.2% formic acid; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001671 |
| Analysis ID: | AN001811 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | UNSPECIFIED |
