Summary of Study ST001257
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000843. The data can be accessed directly via it's Project DOI: 10.21228/M8QH5G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001257 |
Study Title | Nutrimetabolomics and DASH diet |
Study Summary | Although health benefits of the Dietary Approaches to Stop Hypertension (DASH) diet are established, it is not understood which food compounds result in these benefits. We used a step-wise approach to identify unique compounds from individual foods of a DASH-style diet, determined if these Food-Specific Compounds (FSC) are detectable in urine, and then examined relationships between urinary FSC and blood pressure (BP). Nineteen subjects were randomized into 6-week controlled DASH-style diet interventions. Untargeted, LC/MS-based metabolomics was performed on 24-hour urine samples collected before and after each intervention and on 12 representative DASH-style foods. |
Institute | University of Colorado Denver |
Department | Anschutz Medical Campus |
Last Name | Reisdorph |
First Name | Nichole |
Address | 12850 East Montview Blvd, Aurora, CO, 80045, USA |
nichole.reisdorph@ucdenver.edu | |
Phone | 3037249234 |
Submit Date | 2019-09-22 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2020-09-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000843 |
Project DOI: | doi: 10.21228/M8QH5G |
Project Title: | Foodomics Dash Diet |
Project Summary: | Untargeted metabolomics was used to analyze urine and a variety of foods from the Dietary Approaches to Stop Hypertension (DASH) diet in order to understand which food compounds result in health benefits. |
Institute: | University of Colorado Denver |
Department: | Anschutz Medical Campus |
Last Name: | Reisdorph |
First Name: | Nichole |
Address: | 12850 East Montview Blvd, Aurora, CO, 80045, USA |
Email: | nichole.reisdorph@ucdenver.edu |
Phone: | 3037249234 |
Funding Source: | NIH (NIDDK, NCATS, NCRR), National Pork Board, NIH Indiana CCTSI, USDA |
Subject:
Subject ID: | SU001325 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Sample type |
---|---|---|
SA091308 | 02 Apple Juice | Food | Food type:Apple Juice | Pre or Post and food:na |
SA091309 | 05 Apple | Food | Food type:Apple | Pre or Post and food:na |
SA091310 | 12 Beef Tenderloin | Food | Food type:Beef | Pre or Post and food:na |
SA091311 | 03 Blueberries | Food | Food type:Blueberries | Pre or Post and food:na |
SA091312 | 04 Broccoli | Food | Food type:Broccoli | Pre or Post and food:na |
SA091313 | 09 Chicken Tenderloin | Food | Food type:Chicken | Pre or Post and food:na |
SA091314 | 13 Coffee diluted | Food | Food type:Coffee diluted | Pre or Post and food:na |
SA091315 | 14 Coffee Neat | Food | Food type:Coffee neat | Pre or Post and food:na |
SA091316 | 11 coffee | Food | Food type:Coffee | Pre or Post and food:na |
SA091317 | 07 Cucumber | Food | Food type:Cucumber | Pre or Post and food:na |
SA091318 | 01 Grapefruit | Food | Food type:Grapefruit | Pre or Post and food:na |
SA091319 | 08 Peanut Butter 8 ul | Food | Food type:Peanut Butter | Pre or Post and food:na |
SA091320 | 06 Pork Loin | Food | Food type:Pork Loin | Pre or Post and food:na |
SA091321 | 10 Tilapia | Food | Food type:Tilapia | Pre or Post and food:na |
SA091322 | S31-141 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091323 | S31-148 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091324 | S31-107 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091325 | S31-109 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091326 | S31-108 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091327 | S31-114 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091328 | S31-119 Post Po | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091329 | S31-116 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091330 | S31-115 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091331 | S31-120 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091332 | S31-111 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091333 | S31-106 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091334 | S31-144 Post Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091335 | S31-142 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091336 | S31-105 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091337 | S31-145 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091338 | S31-146 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091339 | S31-101 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091340 | S31-102 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Chicken |
SA091341 | S31-111 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091342 | S31-114 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091343 | S31-102 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091344 | S31-146 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091345 | S31-115 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091346 | S31-101 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091347 | S31-142 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091348 | S31-116 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091349 | S31-109 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091350 | S31-108 Post Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091351 | S31-141 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091352 | S31-107 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091353 | S31-148 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091354 | S31-105 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091355 | S31-145 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091356 | S31-119 Post-Ch | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091357 | S31-106 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091358 | S31-144 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091359 | S31-120 Post-Po | Urine | Food type:urine | Pre or Post and food:Post Pork |
SA091360 | S31-141 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091361 | S31-142 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091362 | S31-120 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091363 | S31-145 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091364 | S31-119 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091365 | S31-108 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091366 | S31-148 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091367 | S31-106 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091368 | S31-105 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091369 | S31-102 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091370 | S31-101 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091371 | S31-107 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091372 | S31-144 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091373 | S31-114 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091374 | S31-115 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091375 | S31-111 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091376 | S31-146 Pre Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091377 | S31-116 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091378 | S31-109 Pre-Ch | Urine | Food type:urine | Pre or Post and food:Pre Chicken |
SA091379 | S31-144 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091380 | S31-146 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091381 | S31-148 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091382 | S31-145 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091383 | S31-111 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091384 | S31-107 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091385 | S31-108 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091386 | S31-106 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091387 | S31-105 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091388 | S31-101 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091389 | S31-102 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091390 | S31-109 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091391 | S31-114 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091392 | S31-120 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091393 | S31-141 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091394 | S31-119 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091395 | S31-116 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091396 | S31-115 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
SA091397 | S31-142 Pre-Po | Urine | Food type:urine | Pre or Post and food:Pre Pork |
Showing results 1 to 90 of 90 |
Collection:
Collection ID: | CO001319 |
Collection Summary: | Twenty-four-hour urine collections were obtained before and near the end of each of the two DASH-style diet periods. A total of 76 urine samples were used for untargeted metabolomics analysis, including 38 collected while the 19 participants consumed their typical diets (19 before each intervention period) and 38 while the DASH-style diet was consumed (19 during each intervention period). For simplicity, these are referred to as pre- and post-diet urine samples. Food was purchased from a local grocery store for metabolomic analyses. All four types of meat included in the diet (pork, chicken, fish, and beef loin) were analyzed. Fruits and vegetables, if not pre-washed, were washed with tap water and prepared with inedible parts (i.e. leaves or peels) removed. Meat and eggs were cooked as instructed to match prescribed cooked weight. |
Sample Type: | Urine |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001340 |
Treatment Summary: | During the 18-week study period, participants initially consumed their self-chosen, unrestricted (typical) diets for two weeks. Participants were then randomly assigned to consume a DASH-style diet with either lean pork or chicken and fish as the predominant sources of dietary protein for six weeks. This time period was followed by a four-week washout period during which participants again consumed their typical diets prior to consuming the DASH-style diet containing the other predominant protein sources. Samples utilized for the current research consisted of 12 individual foods consumed during the DASH-style diet and urine samples collected prior to each intervention and during the final two weeks of each controlled feeding period. |
Sample Preparation:
Sampleprep ID: | SP001333 |
Sampleprep Summary: | Individual food and urine samples were stored at -80 °C prior to sample preparation. Individual foods (including apple juice) were individually dried using a FreeZone 2.5 plus lyophilizer (Labconco, Kansas City, MO, USA). Dried samples were then divided and macerated using a chilled bio-pulverizer (BioSpec Products, Bartlesville, OK). Approximately 50 mg of each sample was measured into chilled microcentrifuge tubes and stored at -80o C prior to further processes and analysis. 10 µL of coffee was diluted with 90 µL of water, vortexed, aliquotted and stored at -80° C until analysis. An aliquot of coffee was also reserved for neat (i.e. unprocessed) analysis, and stored at -80° C until analysis. Freeze-dried foods were removed from -80° C and allowed to thaw on ice. To each sample, 480 μl of chilled methanol, 10 µl each of a hydrophilic spike mix, hydrophilic positive control mix, hydrophobic spike mix and hydrophobic positive control mix were added (see Supplemental Table S1). Samples were gently vortexed for 10 seconds, placed at -80o C for 60 minutes, and then centrifuged 0o C at 18,000 x g for 15 minutes to facilitate protein precipitation. Supernatants were transferred to new microcentrifuge tubes and dried using vacuum centrifugation at 45o C for approximately 60 minutes. Each sample was suspended in 50 µl of 95:5 LC/MS grade water-acetonitrile and gently vortexed for 30 seconds. Following a quick centrifugation, each sample was then divided into replicate 20 µl aliquots, while 10 µl was removed from each sample to generate a pooled QC sample. Samples were stored at -80° C until analysis. Urine samples were analyzed neat (i.e. without any preparation) except that authentic standards were spiked into urine samples to monitor variability in instrument response or batch effects. |
Processing Storage Conditions: | Described in summary |
Combined analysis:
Analysis ID | AN002086 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 6520 |
Column | Agilent Zorbax Rapid Resolution HT (RRHT) SB-AQ (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6520 QTOF |
Ion Mode | POSITIVE |
Units | Abundance (Counts Log2 Transformed) |
Chromatography:
Chromatography ID: | CH001523 |
Instrument Name: | Agilent 6520 |
Column Name: | Agilent Zorbax Rapid Resolution HT (RRHT) SB-AQ (100 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001937 |
Analysis ID: | AN002086 |
Instrument Name: | Agilent 6520 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Agilent 6520 Time-of-Flight (TOF-MS) with dual electrospray ionization (ESI) source, scan rate 2.21 spectra/second, mass range 50-1700 m/z, gas temperature 325 °C, gas flow 12.0 L/min, nebulizer 30 psi, skimmer 60 V, capillary voltage 4000 V, fragmentor 120 V, reference masses 121.050873 and 922.009798 (Reference mix, Agilent Technologies,). |
Ion Mode: | POSITIVE |