Summary of study ST000014

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000013. The data can be accessed directly via it's Project DOI: 10.21228/M8G59C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000014
Study TitleSkeletal Muscle Treatment (palmitate/palmitoyl carnitine)
Study TypeMS analysis
Study SummaryIsolated skelelal muscle mitochondria incubated in the presence of fatty acid oxidation precursors, palmitic acid and palmitoyl carnitine
Institute
University of California, Davis
DepartmentDavis Genome Center
LaboratoryFiehn
Last NameKind
First NameTobias
Address451 E. Health Sci. Drive, Davis, California 95616, USA
Emailtkind@ucdavis.edu
Submit Date2013-05-20
Num Groups12
Total Subjects72
Raw Data AvailableNo
Analysis Type DetailGC-MS
Release Date2013-06-19
Release Version1
Tobias Kind Tobias Kind
https://dx.doi.org/10.21228/M8G59C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000013
Project DOI:doi: 10.21228/M8G59C
Project Title:Skeletal Muscle Treatment
Project Type:MS analysis
Institute:University of California, Davis
Department:Davis Genome Center
Laboratory:Fiehn
Last Name:Fiehn
First Name:Oliver
Address:451 E. Health Sci. Drive, Davis, California 95616, USA
Email:ofiehn@ucdavis.edu

Subject:

Subject ID:SU000030
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:0.25 years
Gender:male
Animal Animal Supplier:University of Ottawa, Canada Mary-Ellen Harper PhD, PI;
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Compartment Minutes Skeletal Muscle Treatment
SA000732103879cytosol 0 palmitic acid 2.4 uM
SA000733103884cytosol 0 palmitic acid 2.4 uM
SA000734103889cytosol 0 palmitic acid 2.4 uM
SA000735103894cytosol 0 palmitic acid 2.4 uM
SA000736103899cytosol 0 palmitic acid 2.4 uM
SA000737103904cytosol 0 palmitic acid 2.4 uM
SA000756104003cytosol 0 palmitic acid 9.6 uM
SA000757104008cytosol 0 palmitic acid 9.6 uM
SA000758104013cytosol 0 palmitic acid 9.6 uM
SA000759104018cytosol 0 palmitic acid 9.6 uM
SA000760104023cytosol 0 palmitic acid 9.6 uM
SA000761104028cytosol 0 palmitic acid 9.6 uM
SA000780104127cytosol 0 palmitoyl carnitine 9.6 uM
SA000781104132cytosol 0 palmitoyl carnitine 9.6 uM
SA000782104137cytosol 0 palmitoyl carnitine 9.6 uM
SA000783104142cytosol 0 palmitoyl carnitine 9.6 uM
SA000784104147cytosol 0 palmitoyl carnitine 9.6 uM
SA000785104152cytosol 0 palmitoyl carnitine 9.6 uM
SA000744103941cytosol 20 palmitic acid 2.4 uM
SA000745103946cytosol 20 palmitic acid 2.4 uM
SA000746103951cytosol 20 palmitic acid 2.4 uM
SA000747103956cytosol 20 palmitic acid 2.4 uM
SA000748103961cytosol 20 palmitic acid 2.4 uM
SA000749103966cytosol 20 palmitic acid 2.4 uM
SA000768104065cytosol 20 palmitic acid 9.6 uM
SA000769104070cytosol 20 palmitic acid 9.6 uM
SA000770104075cytosol 20 palmitic acid 9.6 uM
SA000771104080cytosol 20 palmitic acid 9.6 uM
SA000772104085cytosol 20 palmitic acid 9.6 uM
SA000773104090cytosol 20 palmitic acid 9.6 uM
SA000792104189cytosol 20 palmitoyl carnitine 9.6 uM
SA000793104194cytosol 20 palmitoyl carnitine 9.6 uM
SA000794104199cytosol 20 palmitoyl carnitine 9.6 uM
SA000795104204cytosol 20 palmitoyl carnitine 9.6 uM
SA000796104209cytosol 20 palmitoyl carnitine 9.6 uM
SA000797104214cytosol 20 palmitoyl carnitine 9.6 uM
SA000738103910mito 0 palmitic acid 2.4 uM
SA000739103915mito 0 palmitic acid 2.4 uM
SA000740103920mito 0 palmitic acid 2.4 uM
SA000741103925mito 0 palmitic acid 2.4 uM
SA000742103930mito 0 palmitic acid 2.4 uM
SA000743103935mito 0 palmitic acid 2.4 uM
SA000762104034mito 0 palmitic acid 9.6 uM
SA000763104039mito 0 palmitic acid 9.6 uM
SA000764104044mito 0 palmitic acid 9.6 uM
SA000765104049mito 0 palmitic acid 9.6 uM
SA000766104054mito 0 palmitic acid 9.6 uM
SA000767104059mito 0 palmitic acid 9.6 uM
SA000786104158mito 0 palmitoyl carnitine 9.6 uM
SA000787104163mito 0 palmitoyl carnitine 9.6 uM
SA000788104168mito 0 palmitoyl carnitine 9.6 uM
SA000789104173mito 0 palmitoyl carnitine 9.6 uM
SA000790104178mito 0 palmitoyl carnitine 9.6 uM
SA000791104183mito 0 palmitoyl carnitine 9.6 uM
SA000750103972mito 20 palmitic acid 2.4 uM
SA000751103977mito 20 palmitic acid 2.4 uM
SA000752103982mito 20 palmitic acid 2.4 uM
SA000753103987mito 20 palmitic acid 2.4 uM
SA000754103992mito 20 palmitic acid 2.4 uM
SA000755103997mito 20 palmitic acid 2.4 uM
SA000774104096mito 20 palmitic acid 9.6 uM
SA000775104101mito 20 palmitic acid 9.6 uM
SA000776104106mito 20 palmitic acid 9.6 uM
SA000777104111mito 20 palmitic acid 9.6 uM
SA000778104116mito 20 palmitic acid 9.6 uM
SA000779104121mito 20 palmitic acid 9.6 uM
SA000798104220mito 20 palmitoyl carnitine 9.6 uM
SA000799104225mito 20 palmitoyl carnitine 9.6 uM
SA000800104230mito 20 palmitoyl carnitine 9.6 uM
SA000801104235mito 20 palmitoyl carnitine 9.6 uM
SA000802104240mito 20 palmitoyl carnitine 9.6 uM
SA000803104245mito 20 palmitoyl carnitine 9.6 uM
Showing results 1 to 72 of 72

Collection:

Collection ID:CO000014
Sample Type:Tissue
Collection Method:isolated skelelal muscle mitochondria incubated in the presence of fatty acid oxidation precursors.
Collection Time:9/4/06
Volumeoramount Collected:1 mg

Treatment:

Treatment ID:TR000022
Treatment Compound:palmitic acid 2.4 uM | palmitic acid 9.6 uM | palmitoyl carnitine 9.6 uM

Sample Preparation:

Sampleprep ID:SP000027
Sampleprep Protocol ID:Mitochondrial Matrix Preparation
Extraction Method:Extramitochondrial Buffer Preparation: The Harper lab first entrifuged mitochondrial preparations in order to get the extramitochondrial buffer and mitochondrial pellet. Mitochondrial Matrix Preparation: the mitochondrial matrix was exposed by repeated freeze-thaws of the mitochondrial pellet; the insoluble material was spun down, and supernatant collected as matrix. Pellets were resuspended in 100 ul of buffer (140 mM KCL, 20 HEPES, 5 K2HPO4, 1 EGTA, pH 7.4 at RT)
Organ:skeletal muscle
Organ Specification:total
Cell Type:myocyte
Subcellular Location:extramitochondrial buffer

Combined analysis:

Analysis ID AN000031
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus III GC TOF
Ion Mode POSITIVE
Units Peak height

Chromatography:

Chromatography ID:CH000014
Instrument Name:Agilent 6890N
Chromatography Type:GC

MS:

MS ID:MS000030
Analysis ID:AN000031
Instrument Name:Leco Pegasus III GC TOF
Instrument Type:GC-TOF
MS Type:EI
Ion Mode:POSITIVE
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