Summary of study ST000015

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000013. The data can be accessed directly via it's Project DOI: 10.21228/M8G59C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000015
Study TitleSkeletal Muscle Treatment (palmitate and inhibitors)
Study TypeMS analysis
Study SummaryIsolated skelelal muscle mitochondria incubated in the presence of fatty acid oxidation precursor and inhibitors
Institute
University of California, Davis
DepartmentDavis Genome Center
LaboratoryFiehn
Last NameKind
First NameTobias
Address451 E. Health Sci. Drive, Davis, California 95616, USA
Emailtkind@ucdavis.edu
Submit Date2013-05-20
Num Groups20
Total Subjects125
Raw Data AvailableNo
Analysis Type DetailGC-MS
Release Date2013-06-19
Release Version1
Tobias Kind Tobias Kind
https://dx.doi.org/10.21228/M8G59C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000013
Project DOI:doi: 10.21228/M8G59C
Project Title:Skeletal Muscle Treatment
Project Type:MS analysis
Institute:University of California, Davis
Department:Davis Genome Center
Laboratory:Fiehn
Last Name:Fiehn
First Name:Oliver
Address:451 E. Health Sci. Drive, Davis, California 95616, USA
Email:ofiehn@ucdavis.edu

Subject:

Subject ID:SU000031
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL6/J
Age Or Age Range:0.4 years
Gender:Female
Animal Animal Supplier:University of Ottawa, Vivarium.
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Compartment Minutes Skeletal Muscle Treatment
SA000903332577buffer 0 19 uM palmitate
SA000904332581buffer 0 19 uM palmitate
SA000905332585buffer 0 19 uM palmitate
SA000906332589buffer 0 19 uM palmitate
SA000907332593buffer 0 19 uM palmitate
SA000908332597buffer 0 19 uM palmitate
SA000909332601buffer 0 19 uM palmitate
SA000880332469buffer 0 2 uM palmitate
SA000881332477buffer 0 2 uM palmitate
SA000882332481buffer 0 2 uM palmitate
SA000883332485buffer 0 2 uM palmitate
SA000884332489buffer 0 2 uM palmitate
SA000858332353buffer 0 9 uM palmitate
SA000859332361buffer 0 9 uM palmitate
SA000860332365buffer 0 9 uM palmitate
SA000861332369buffer 0 9 uM palmitate
SA000862332373buffer 0 9 uM palmitate
SA000830332241buffer 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000831332245buffer 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000832332249buffer 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000833332253buffer 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000834332257buffer 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000835332261buffer 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000836332265buffer 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000804332129buffer 0 ethanol only
SA000805332133buffer 0 ethanol only
SA000806332137buffer 0 ethanol only
SA000807332141buffer 0 ethanol only
SA000808332145buffer 0 ethanol only
SA000809332149buffer 0 ethanol only
SA000810332153buffer 0 ethanol only
SA000916332633buffer 20 19 uM palmitate
SA000917332637buffer 20 19 uM palmitate
SA000918332641buffer 20 19 uM palmitate
SA000919332645buffer 20 19 uM palmitate
SA000920332649buffer 20 19 uM palmitate
SA000921332653buffer 20 19 uM palmitate
SA000922332657buffer 20 19 uM palmitate
SA000891332525buffer 20 2 uM palmitate
SA000892332529buffer 20 2 uM palmitate
SA000893332533buffer 20 2 uM palmitate
SA000894332537buffer 20 2 uM palmitate
SA000895332541buffer 20 2 uM palmitate
SA000896332545buffer 20 2 uM palmitate
SA000870332409buffer 20 9 uM palmitate
SA000871332413buffer 20 9 uM palmitate
SA000872332417buffer 20 9 uM palmitate
SA000873332421buffer 20 9 uM palmitate
SA000874332429buffer 20 9 uM palmitate
SA000844332297buffer 20 9 uM palmitate+inhibitors (rotenone+malonate)
SA000845332301buffer 20 9 uM palmitate+inhibitors (rotenone+malonate)
SA000846332305buffer 20 9 uM palmitate+inhibitors (rotenone+malonate)
SA000847332309buffer 20 9 uM palmitate+inhibitors (rotenone+malonate)
SA000848332313buffer 20 9 uM palmitate+inhibitors (rotenone+malonate)
SA000849332317buffer 20 9 uM palmitate+inhibitors (rotenone+malonate)
SA000850332321buffer 20 9 uM palmitate+inhibitors (rotenone+malonate)
SA000817332185buffer 20 ethanol only
SA000818332189buffer 20 ethanol only
SA000819332193buffer 20 ethanol only
SA000820332197buffer 20 ethanol only
SA000821332201buffer 20 ethanol only
SA000822332205buffer 20 ethanol only
SA000823332209buffer 20 ethanol only
SA000910332605matrix 0 19 uM palmitate
SA000911332609matrix 0 19 uM palmitate
SA000912332613matrix 0 19 uM palmitate
SA000913332617matrix 0 19 uM palmitate
SA000914332625matrix 0 19 uM palmitate
SA000915332629matrix 0 19 uM palmitate
SA000885332493matrix 0 2 uM palmitate
SA000886332497matrix 0 2 uM palmitate
SA000887332501matrix 0 2 uM palmitate
SA000888332505matrix 0 2 uM palmitate
SA000889332509matrix 0 2 uM palmitate
SA000890332513matrix 0 2 uM palmitate
SA000863332381matrix 0 9 uM palmitate
SA000864332385matrix 0 9 uM palmitate
SA000865332389matrix 0 9 uM palmitate
SA000866332393matrix 0 9 uM palmitate
SA000867332397matrix 0 9 uM palmitate
SA000868332401matrix 0 9 uM palmitate
SA000869332405matrix 0 9 uM palmitate
SA000837332269matrix 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000838332273matrix 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000839332277matrix 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000840332281matrix 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000841332285matrix 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000842332289matrix 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000843332293matrix 0 9 uM palmitate+inhibitors (rotenone+malonate)
SA000811332157matrix 0 ethanol only
SA000812332161matrix 0 ethanol only
SA000813332165matrix 0 ethanol only
SA000814332169matrix 0 ethanol only
SA000815332173matrix 0 ethanol only
SA000816332181matrix 0 ethanol only
SA000923332661matrix 20 19 uM palmitate
SA000924332665matrix 20 19 uM palmitate
SA000925332669matrix 20 19 uM palmitate
SA000926332673matrix 20 19 uM palmitate
SA000927332677matrix 20 19 uM palmitate
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Collection:

Collection ID:CO000015
Sample Type:Tissue
Collection Method:Isolated skelelal muscle mitochondria incubated in the presence of fatty acid oxidation precursor and inhibitors
Collection Time:4/19/07
Volumeoramount Collected:1 mg

Treatment:

Treatment ID:TR000025
Treatment Compound:ethanol only | 9 uM palmitate+inhibitors (rotenone+malonate) | 9 uM palmitate | 2 uM palmitate | 19 uM palmitate |

Sample Preparation:

Sampleprep ID:SP000028
Sampleprep Protocol ID:Extramitochondrial Buffer Preparation
Extraction Method:The Harper lab first entrifuged mitochondrial preparations in order to get the extramitochondrial buffer and mitochondrial pellet. Mitochondrial Matrix Preparation: the mitochondrial matrix was exposed by repeated freeze-thaws of the mitochondrial pellet; the insoluble material was spun down, and supernatant collected as matrix.
Organ:skeletal muscle
Organ Specification:total
Cell Type:myocyte
Subcellular Location:extramitochondrial buffer

Combined analysis:

Analysis ID AN000032
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus III GC TOF
Ion Mode POSITIVE
Units Peak height

Chromatography:

Chromatography ID:CH000015
Instrument Name:Agilent 6890N
Chromatography Type:GC

MS:

MS ID:MS000031
Analysis ID:AN000032
Instrument Name:Leco Pegasus III GC TOF
Instrument Type:GC-TOF
MS Type:EI
Ion Mode:POSITIVE
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