Summary of study ST000019

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000018. The data can be accessed directly via it's Project DOI: 10.21228/M8Z59Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000019
Study TitleDetermine purity and quality of IROA labelled glucose
Study Type NMR and MS analysis
Study SummaryWe demonstrate the global metabolic analysis ofCaenorhabditis elegansstress responses using a mass-spectrometry-based technique called isotopic ratio outlier analysis (IROA). In an IROA protocol, control and experimental samples are isotopically labeled with 95 and 5%13C, and the two sample populations are mixed together for uniform extraction, sample preparation, and LC-MS analysis.To illustrate the utility of IROA for global metabolomics, we exposed wild-type (N2) worms to a heat shock (30 min heat shock at 33 C), which causes significant, widespread changes in metabolism. We collected and analyzed material from the exometabolome (all material that worms release in the supernatant) and the endometabolome (homogenized total extracts from the worm bodies).
Institute
University of Florida
DepartmentBiochemistry & Molecular Biology
Last NameStupp
First NameGregory
Emailstuppie@ufl.edu
Submit Date2013-12-10
Study CommentsDetermine purity and quality of IROA labelled glucose
Raw Data AvailableYes
Raw Data File Type(s).par.temp,.txt,.mnova, binary data
Uploaded File Size90 M
Analysis Type DetailNMR
Release Date2014-03-05
Release Version1
Gregory Stupp Gregory Stupp
https://dx.doi.org/10.21228/M8Z59Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000018
Project DOI:doi: 10.21228/M8Z59Q
Project Title:Isotopic Ratio Outlier Analysis Global Metabolomics ofCaenorhabditis elegans
Project Type:IROA labeling study
Project Summary:We demonstrate the global metabolic analysis ofCaenorhabditis elegansstress responses using a mass-spectrometry-based technique called isotopic ratio outlier analysis (IROA). In an IROA protocol, control and experimental samples are isotopically labeled with 95 and 5%13C, and the two sample populations are mixed together for uniform extraction, sample preparation, and LC-MS analysis.To illustrate the utility of IROA for global metabolomics, we exposed wild-type (N2) worms to a heat shock (30 min heat shock at 33 C), which causes significant, widespread changes in metabolism. We collected and analyzed material from the exometabolome (all material that worms release in the supernatant) and the endometabolome (homogenized total extracts from the worm bodies).
Institute:University of Florida
Department:Biochemistry & Molecular Biology
Laboratory:Edison
Last Name:Edison
First Name:Arthur
Address:R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Email:aedison@ufl.edu
Phone:(352) 392-4535
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