Summary of study ST000045

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000044. The data can be accessed directly via it's Project DOI: 10.21228/M8D59R This work is supported by NIH grant, U2C- DK119886.

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Study IDST000045
Study TitlePlasma metabolomics: Comparison of non-diabetic controls with T1D patients
Study TypeDrug effect study
Study SummaryNon-diabetic controls whose metabolites were compared to T1D patients with and without insulin. Seven C-peptide┬ľnegative T1D subjects were studied on two occasions: one during insulin treatment and the other following withdrawal of insulin for 8 h and compared with matched healthy ND participants
Institute
Mayo Clinic
DepartmentEndocrinology
Last NameNair
First NameSreekumaran
EmailDasari.Surendra@mayo.edu
Submit Date2014-03-24
Num Groups1
Total Subjects7
Raw Data AvailableYes
Raw Data File Type(s).bin,.xml,.stg,.m
Uploaded File Size66 G
Analysis Type DetailLC-MS
Release Date2014-04-20
Release Version1
Sreekumaran Nair Sreekumaran Nair
https://dx.doi.org/10.21228/M8D59R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000044
Project DOI:doi: 10.21228/M8D59R
Project Title:Concordance of changes in metabolic pathways based on plasma metabolomics and skeletal muscle transcriptomics in type 1 diabetes.
Project Type:Untargeted LC-MS Metabolomics
Project Summary:Insulin regulates many cellular processes, but the full impact of insulin deficiency on cellular functions remains to be defined. Applying a mass spectrometry-based nontargeted metabolomics approach, we report here alterations of 330 plasma metabolites representing 33 metabolic pathways during an 8-h insulin deprivation in type 1 diabetic individuals. These pathways included those known to be affected by insulin such as glucose, amino acid and lipid metabolism, Krebs cycle, and immune responses and those hitherto unknown to be altered including prostaglandin, arachidonic acid, leukotrienes, neurotransmitters, nucleotides, and anti-inflammatory responses. A significant concordance of metabolome and skeletal muscle transcriptome-based pathways supports an assumption that plasma metabolites are chemical fingerprints of cellular events. Although insulin treatment normalized plasma glucose and many other metabolites, there were 71 metabolites and 24 pathways that differed between nondiabetes and insulin-treated type 1 diabetes. Confirmation of many known pathways altered by insulin using a single blood test offers confidence in the current approach. Future research needs to be focused on newly discovered pathways affected by insulin deficiency and systemic insulin treatment to determine whether they contribute to the high morbidity and mortality in T1D despite insulin treatment.
Institute:Mayo Clinic
Department:Endocrinology
Last Name:Nair
First Name:Sreekumaran
Address:200 First Street SW, Rochester, MN 55905
Email:Dasari.Surendra@mayo.edu
Phone:507-284-0513
Funding Source:R01 DK41973, UL1 RR024150-01
Project Comments:22415876
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