Summary of study ST000145

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000145
Study TitlePrimary T Cell Noxa Knockdown (Donor 8)
Study SummaryCells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 12 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-03-13
Num Groups3
Total Subjects15
Raw Data AvailableYes
Raw Data File Type(s).cd,.cG, .xml, .bin,.stg
Uploaded File Size3.8 G
Analysis Type DetailGC/LC-MS
Release Date2015-03-13
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M85C7W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000129
Project DOI:doi: 10.21228/M85C7W
Project Title:Metabolic effects of Noxa
Project Summary:labeled glutamine metabolomics on Noxa expressing cells
Institute:University of Minnesota
Department:Masonic Cancer Center
Laboratory:Kelekar Lab
Last Name:Kelekar
First Name:Ameeta
Address:420 Delaware Street SE, Minneapolis, MN 55455
Email:ameeta@umn.edu
Phone:612-626-2358

Subject:

Subject ID:SU000164
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Label
SA008028S00016916Glucose
SA008029S00016929Glucose
SA008030S00016921Glucose
SA008031S00016920Glucose
SA008032S00016918Glucose
SA008033S00016919Glucose
SA008034S00016917Glucose
SA008035S00016928Glutamine
SA008036S00016930Glutamine
SA008037S00016927Glutamine
SA008038S00016923Glutamine
SA008039S00016922Glutamine
SA008040S00016924Glutamine
SA008041S00016925Glutamine
SA008042S00016926Glutamine
Showing results 1 to 15 of 15

Collection:

Collection ID:CO000150
Sample Type:Cells

Treatment:

Treatment ID:TR000169

Sample Preparation:

Sampleprep ID:SP000164
Sampleprep Protocol Filename:Fluxomics_analysis_protocol-2015-03-11.pdf

Combined analysis:

Analysis ID AN000230 AN000231
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Agilent 1260 LCLuna 3um NH2 100 , LC Column 150 x 1 mm, Ea (Phenomenex Inc.) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6520 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units Area Area

Chromatography:

Chromatography ID:CH000160
Methods ID:AQM010|AQM011
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m.zip
ALPHA_KETO_ACIDS-FULL.M.zip
Instrument Name:Agilent 1260
Column Name:Agilent 1260 LCLuna 3um NH2 100 , LC Column 150 x 1 mm, Ea (Phenomenex Inc.)
Chromatography Type:HILIC
  
Chromatography ID:CH001300
Methods ID:AQM011
Methods Filename:ALPHA_KETO_ACIDS-FULL.M.zip
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000181
Analysis ID:AN000230
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000182
Analysis ID:AN000231
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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