Summary of study ST000193

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000164. The data can be accessed directly via it's Project DOI: 10.21228/M81G69 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000193
Study TitleBAF60a LKO Liver specific knockout Bile acid
Study SummaryTwelve weeks old WT and BAF60a Liver specfific knockout mice were fed with high cholesterol diet for 8 weeks. Mice were sacrified after 4 h of starvation. Blood samples were harvested by heart puncture for plasma. Liver were dissected and immediately frozen in liquid nitrogen. All the samples were kept in -80oC freezer for long term storage
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-09
Num Groups2
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s).xml,.xsd,.stg, .bin, .cd,.cG
Uploaded File Size64 M
Analysis Type DetailLC-MS
Release Date2015-12-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M81G69
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000164
Project DOI:doi: 10.21228/M81G69
Project Title:BAF60a LKO Liver specific knockout Bile acid
Project Summary:Regulation of Bile acid metabolism by hepatic BAF60a
Institute:University of Michigan
Department:Life Sciences Institute
Laboratory:Lin Lab
Last Name:Lin
First Name:Jiandie
Address:Ann Arbor, MI
Email:jdlin@umich.edu
Phone:734-615-3512

Subject:

Subject ID:SU000212
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA009748S00014262Knockout
SA009749S00014253Knockout
SA009750S00014267Knockout
SA009751S00014268Knockout
SA009752S00014270Knockout
SA009753S00014259Knockout
SA009754S00014261Knockout
SA009755S00014258Knockout
SA009756S00014256Wild type
SA009757S00014255Wild type
SA009758S00014254Wild type
SA009759S00014266Wild type
SA009760S00014269Wild type
SA009761S00014265Wild type
SA009762S00014257Wild type
SA009763S00014263Wild type
SA009764S00014264Wild type
SA009765S00014260Wild type
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000200
Sample Type:Liver

Treatment:

Treatment ID:TR000220

Sample Preparation:

Sampleprep ID:SP000214
Sampleprep Protocol Filename:Bile-acids-2015-03-11.docx

Combined analysis:

Analysis ID AN000295
Analysis type MS
Chromatography type Reversed phase
Chromatography system
Column Waters Acquity BEH C18 (50 x 2.1mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode NEGATIVE
Units nmol

Chromatography:

Chromatography ID:CH000216
Methods ID:AQM040
Methods Filename:2014-03-27-Bile_acid-full-ESN-insert.m.zip
Column Name:Waters Acquity BEH C18 (50 x 2.1mm, 1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000244
Analysis ID:AN000295
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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