Summary of study ST000274

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000218. The data can be accessed directly via it's Project DOI: 10.21228/M86W2G This work is supported by NIH grant, U2C- DK119886.

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Study IDST000274
Study TitleHIF 1 alpha type 2 cells metabolomics
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study SummaryLung contusion is a major risk factor for the development of acute respiratory distress syndrome. Hypoxia-inducible factor-1? is the primary transcription factor that is responsible for regulating the cellular response to changes in oxygen tension. We set to determine if hypoxia-inducible factor-1? plays a role in the pathogenesis of acute inflammatory response and injury in lung contusion. Nonlethal closed-chest unilateral lung contusion was induced in a hypoxia reporter mouse model and type 2 cell-specific hypoxia-inducible factor-1? conditional knockout mice. The mice were killed at 5-, 24-, 48-, and 72-hour time points, and the extent of systemic and tissue hypoxia was assessed. In addition, injury and inflammation were assessed by measuring bronchoalveolar lavage cells (flow cytometry and cytospin), albumin (permeability injury), and cytokines (inflammation). Isolated type 2 cells from the hypoxia-inducible factor-1? conditional knockout mice were isolated and evaluated for proinflammatory cytokines following lung contusion. Finally, the role of nuclear factor-?B and interleukin-1? as intermediates in this interaction was studied. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245055/
Institute
University of Michigan
DepartmentSurgery
LaboratoryRaghavendran Lab
Last NameThomas
First NameBivin
AddressAnn Arbor, MI
Emailbthomas@umich.edu
Phone734-615-7142
Submit Date2014-06-04
Num Groups4
Total Subjects8
Study Commentshttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245055/ : HIF1 (+/+) stands for hypoxia reporter mouse model
HIF1 (-/-) stands for type 2 cell-specific hypoxia-inducible factor-1? conditional knockout
Analysis Type DetailLC-MS
Release Date2016-01-13
Release Version1
Bivin Thomas Bivin Thomas
https://dx.doi.org/10.21228/M86W2G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000218
Project DOI:doi: 10.21228/M86W2G
Project Title:HIF 1 alpha
Project Summary:Glycolysis/TCA/Nucleotide analysis (AEC cells)
Institute:University of Michigan
Department:Surgery
Laboratory:Raghavendran Lab
Last Name:Raghavendran
First Name:Krishnan
Address:Ann Arbor, MI
Email:kraghave@umich.edu
Phone:734-615-7142

Subject:

Subject ID:SU000294
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Treatment
SA012209S00014949HIF1 (-/-) Control
SA012210S00014948HIF1 (-/-) Control
SA012205S00014944HIF1 (+/+) Control
SA012206S00014945HIF1 (+/+) Control
SA012211S00014950HIF1 (-/-) Lung contusion, 24h
SA012212S00014951HIF1 (-/-) Lung contusion, 24h
SA012207S00014947HIF1 (+/+) Lung contusion, 24h
SA012208S00014946HIF1 (+/+) Lung contusion, 24h
SA012213S00020491Pooled sample Pooled cells
Showing results 1 to 9 of 9

Collection:

Collection ID:CO000288
Sample Type:Cells

Treatment:

Treatment ID:TR000308

Sample Preparation:

Sampleprep ID:SP000302
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000437
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode NEGATIVE
Units pmol/µg protein

Chromatography:

Chromatography ID:CH000306
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS000378
Analysis ID:AN000437
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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