Summary of study ST000279

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000222. The data can be accessed directly via it's Project DOI: 10.21228/M8TP4R This work is supported by NIH grant, U2C- DK119886.

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Study IDST000279
Study TitleViral Effect on Metabolism (part II)
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study SummarySmall airway epithelial cell (SAEC) (Lonza Walkersville, Inc., MD) were grown according to the manufacturer's instructions in growth medium (SAGM) containing 0.03 mg/ml bovine pituitary extract (BPE), 0.5 µg/ml hydrocortisone, 0.5 ng/ml hEGF, 0.5 µg/ml epinephrine, 10 µg/ml transferrin, 5 µg/ml insulin, 0.1 ng/ml retinoic acid, 6.5ng/ml triiodothyronine, 50 µg/ml gentamicin and 50ng/ml Amphotericin-B, and 0.5 mg/ml bovine serum albumin (BSA, fatty acid free). When SAE grew to around 80 to 90% confluence, the cells were put into basal medium not supplemented with growth factors 3hours. Then the cell monolayers were infected with naïve hMPV at multiplicity of infection (MOI) of 3 to certain time. The supernatant was saved and cells were harvested and stored exactly according to the protocol provided by University of Michigan Metabolomics Core Facility for metabolomics analysis. 50mM ammonium acetate in water was used for rinse buffer.
Institute
University of Michigan
DepartmentDepartment of Pediatrics
LaboratoryCasola Lab (University of Texas Medical Branch)
Last NameZhao
First NameYanhua
AddressGalveston, TX
Emailyazhao1@utmb.edu
Phone409-443-1461
Submit Date2015-02-05
Num Groups4
Total Subjects24
Analysis Type DetailGC/LC-MS
Release Date2016-01-13
Release Version1
Yanhua Zhao Yanhua Zhao
https://dx.doi.org/10.21228/M8TP4R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000222
Project DOI:doi: 10.21228/M8TP4R
Project Title:Viral Effect on Metabolism
Project Type:Glycolysis/TCA/Nucleotide analysis (tissue/cells)
Project Summary:Global Metabolic profiling of infection by respiratory virus
Institute:University of Texas
Department:Department of Pediatrics
Laboratory:Casola Lab
Last Name:Casola
First Name:Antonella
Address:Galveston, TX
Email:yazhao1@utmb.edu
Phone:409-772-2798

Subject:

Subject ID:SU000299
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Time Diseased
SA012255S00017199Naïve hMPV 0hr
SA012256S00017200Naïve hMPV 0hr
SA012257S00017201Naïve hMPV 0hr
SA012258S00017206Naïve hMPV 15hrs
SA012259S00017205Naïve hMPV 15hrs
SA012260S00017207Naïve hMPV 15hrs
SA012261S00017209Naïve hMPV 24hrs
SA012262S00017210Naïve hMPV 24hrs
SA012263S00017208Naïve hMPV 24hrs
SA012264S00017203Naïve hMPV 6hrs
SA012265S00017202Naïve hMPV 6hrs
SA012266S00017204Naïve hMPV 6hrs
SA012267S00017188RSV 0hr
SA012268S00017189RSV 0hr
SA012269S00017187RSV 0hr
SA012270S00017195RSV 15hrs
SA012271S00017193RSV 15hrs
SA012272S00017194RSV 15hrs
SA012273S00017198RSV 24hrs
SA012274S00017197RSV 24hrs
SA012275S00017196RSV 24hrs
SA012276S00017190RSV 6hrs
SA012277S00017192RSV 6hrs
SA012278S00017191RSV 6hrs
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000293
Sample Type:Cells, Cultured

Treatment:

Treatment ID:TR000313

Sample Preparation:

Sampleprep ID:SP000307
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000445 AN000446
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6530 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units pmol/µg protein pmol/µg protein

Chromatography:

Chromatography ID:CH000316
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001309
Methods ID:AQM011
Methods Filename:ALPHA_KETO_ACIDS-FULL.M.zip
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000386
Analysis ID:AN000445
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000387
Analysis ID:AN000446
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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