Summary of study ST000282

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000224. The data can be accessed directly via it's Project DOI: 10.21228/M8K599 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000282
Study TitlePilot Study 13C flux effects when RhoC or RhoA perturbed (13C BCs)
Study Type13C mass isotopomer analysis (flux studies)
Study SummaryRho-GTPases are small GTP-binding proteins that contribute to the epithelial-to-mesenchymal transition by regulating several cellular processes including organization of the actin cytoskeleton, cell motility, transcription, and cell proliferation. Overexpression of RhoC-GTPases (RhoC) in breast cancer has been implicated in poor disease prognosis due to increased cancer cells invasion, migration, and motility, which warranted its consideration as a therapeutic target for inhibiting breast cancer metastasis. Using silencing RNA (siRNA) molecules to knockdown RhoC expression is a promising approach to inhibit breast cancer metastases.
Institute
University of Michigan
DepartmentInternal Medicine
LaboratoryMerajver Lab
Last NameWynn
First NameMichelle
AddressUniversity Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Emailmlwynn@umich.edu
Phone734-647-3408
Submit Date2015-02-11
Num Groups5
Total Subjects16
Analysis Type DetailGC/LC-MS
Release Date2016-01-13
Release Version1
Michelle Wynn Michelle Wynn
https://dx.doi.org/10.21228/M8K599
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000224
Project DOI:doi: 10.21228/M8K599
Project Title:13C mass isotopomer analysis
Project Summary:Pilot studies before designing future experiments
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Merajver Lab
Last Name:Merajver
First Name:Sophia
Address:University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Email:smerajve@med.umich.edu
Phone:(734)936-6884

Subject:

Subject ID:SU000302
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line 13C Label RhoC overexpression RhoA knockdown RhoC knockdown RhoC drug
SA012306S00017282MCF10A  Glutamine no no no no
SA012307S00017283MCF10A  Glutamine yes no no no
SA012308S00017284MDA-MB-231 Glutamine no no no no
SA012309S00017287MDA-MB-231 Glutamine no no no yes
SA012310S00017286MDA-MB-231 Glutamine no no yes no
SA012311S00017285MDA-MB-231 Glutamine no yes no no
SA012312S00017295SUM149 Glucose no no no no
SA012313S00017293SUM149 Glucose no no no no
SA012314S00017294SUM149 Glucose no no no yes
SA012315S00017290SUM149 Glutamine no no no no
SA012316S00017288SUM149 Glutamine no no no no
SA012317S00017289SUM149 Glutamine no no no yes
SA012318S00017296SUM190 Glucose no no no no
SA012319S00017297SUM190 Glucose no no yes no
SA012320S00017291SUM190 Glutamine no no no no
SA012321S00017292SUM190 Glutamine no no yes no
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000296
Sample Type:Cell Line

Treatment:

Treatment ID:TR000316

Sample Preparation:

Sampleprep ID:SP000310
Sampleprep Protocol Filename:Fluxomics_analysis_protocol-2015-03-11.docx

Combined analysis:

Analysis ID AN000449 AN000450
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6530 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units Area Area normalized

Chromatography:

Chromatography ID:CH000320
Methods ID:AQM010
Methods Filename:QTOF-002-HILIC-35min-1mm.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001310
Methods ID:AQM012
Methods Filename:MAHMOUD-PALM_14ISOTOPE.M
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000390
Analysis ID:AN000449
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000391
Analysis ID:AN000450
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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