Summary of Study ST000309
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000248. The data can be accessed directly via it's Project DOI: 10.21228/M8QS3G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000309 |
| Study Title | Metabolome of three Vancomycin-intermediate Staphylococcus aureus (VISA) mutants compared with the parent strain MM66 |
| Study Summary | Vancomycin-intermediate Staphylococcus aureus (VISA) evolve in a strain-specific manner and acquire mutations that lead to alterations in cell wall metabolism that reduce susceptibility to vancomycin. We had earlier isolated several VISA mutant strains of the clinical hVISA strain MM66. This study is aimed at analyzing the metabolome of these mutants in comparison to the parent strain. |
| Institute | Oklahoma State University |
| Last Name | Gustafson |
| First Name | John |
| Address | 246C Noble Research Center Oklahoma State University Stillwater, OK 74078-3035 |
| john.gustafson@okstate.edu | |
| Phone | 405-744-6189 |
| Submit Date | 2016-01-12 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2016-01-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000248 |
| Project DOI: | doi: 10.21228/M8QS3G |
| Project Title: | Metabolome analysis of vancomycin-intermediate Staphylococcus aureus (VISA) strains |
| Project Summary: | GC/MS analysis of metabolites isolated from VISA mutants of Staphylococcus aureus hVISA strain MM66, compared to its parent strain MM66 |
| Institute: | Oklahoma State University |
| Last Name: | Gustafson |
| First Name: | John |
| Address: | 246C Noble Research Center Oklahoma State University Stillwater, OK 74078-3035 |
| Email: | john.gustafson@okstate.edu |
| Phone: | 405-744-6189 |
Subject:
| Subject ID: | SU000329 |
| Subject Type: | Bacterial cells |
| Subject Species: | Staphylococcus aureus |
| Taxonomy ID: | 1280 |
| Species Group: | Microorganism |
Factors:
Subject type: Bacterial cells; Subject species: Staphylococcus aureus (Factor headings shown in green)
| mb_sample_id | local_sample_id | source | type |
|---|---|---|---|
| SA014035 | MM66-2S1 | mutant | VISA2 |
| SA014040 | MM66-2S2 | mutant | VISA2 |
| SA014042 | MM66-2S3 | mutant | VISA2 |
| SA014038 | MM66-3S3 | mutant | VISA3 |
| SA014041 | MM66-3S2 | mutant | VISA3 |
| SA014043 | MM66-3S1 | mutant | VISA3 |
| SA014036 | MM66-4S2 | mutant | VISA4 |
| SA014037 | MM66-4S3 | mutant | VISA4 |
| SA014039 | MM66-4S1 | mutant | VISA4 |
| SA014044 | MM66-S3 | parent | hVISA |
| SA014045 | MM66-S1 | parent | hVISA |
| SA014046 | MM66-S2 | parent | hVISA |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO000323 |
| Collection Summary: | MM66 VISA selected on 3 mg/l vancomycin |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR000343 |
| Treatment Summary: | MM66 VISA selected on 3 mg/l vancomycin |
Sample Preparation:
| Sampleprep ID: | SP000337 |
| Sampleprep Summary: | The sample extraction was referred to the procedure of Roessner et al. (2000, 2001) with minor changes: About 10 mg (DW) of S. aureus cells were crashed in the mill (Model, Manufacturer), and then sonicated with the addition of 1.5 ml of 70% methanol for 10 min at 600C, followed by extraction with 1.5 ml water at room temperature. Both extracts were combined and dried in vacuum. Prior to GC/MS analysis, dried polar extracts were derivatized with 80 µl Methoxyamine hydrochloride (20mg ml-1) for 60 min at 400C and then with 80 µl MSTFA at 650C for 40 min. |
Combined analysis:
| Analysis ID | AN000489 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Agilent HP5-MS |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | relative concentration/10mg DW |
Chromatography:
| Chromatography ID: | CH000349 |
| Instrument Name: | Agilent 6890N |
| Column Name: | Agilent HP5-MS |
| Flow Rate: | 1.3 ml min-1 |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000427 |
| Analysis ID: | AN000489 |
| Instrument Name: | Agilent 5973 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 230C |
| Scanning Range: | 50-800 m/z |
