Summary of study ST000309

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000248. The data can be accessed directly via it's Project DOI: 10.21228/M8QS3G This work is supported by NIH grant, U2C- DK119886.

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Study IDST000309
Study TitleMetabolome of three Vancomycin-intermediate Staphylococcus aureus (VISA) mutants compared with the parent strain MM66
Study SummaryVancomycin-intermediate Staphylococcus aureus (VISA) evolve in a strain-specific manner and acquire mutations that lead to alterations in cell wall metabolism that reduce susceptibility to vancomycin. We had earlier isolated several VISA mutant strains of the clinical hVISA strain MM66. This study is aimed at analyzing the metabolome of these mutants in comparison to the parent strain.
Institute
Oklahoma State University
Last NameGustafson
First NameJohn
Address246C Noble Research Center Oklahoma State University Stillwater, OK 74078-3035
Emailjohn.gustafson@okstate.edu
Phone405-744-6189
Submit Date2016-01-12
Analysis Type DetailGC-MS
Release Date2016-01-14
Release Version1
John Gustafson John Gustafson
https://dx.doi.org/10.21228/M8QS3G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000248
Project DOI:doi: 10.21228/M8QS3G
Project Title:Metabolome analysis of vancomycin-intermediate Staphylococcus aureus (VISA) strains
Project Summary:GC/MS analysis of metabolites isolated from VISA mutants of Staphylococcus aureus hVISA strain MM66, compared to its parent strain MM66
Institute:Oklahoma State University
Last Name:Gustafson
First Name:John
Address:246C Noble Research Center Oklahoma State University Stillwater, OK 74078-3035
Email:john.gustafson@okstate.edu
Phone:405-744-6189

Subject:

Subject ID:SU000329
Subject Type:Bacterial cells
Subject Species:Staphylococcus aureus
Taxonomy ID:1457394
Species Group:Microorganism

Factors:

Subject type: Bacterial cells; Subject species: Staphylococcus aureus (Factor headings shown in green)

mb_sample_id local_sample_id source type
SA014035MM66-2S1mutant VISA2
SA014040MM66-2S2mutant VISA2
SA014042MM66-2S3mutant VISA2
SA014038MM66-3S3mutant VISA3
SA014041MM66-3S2mutant VISA3
SA014043MM66-3S1mutant VISA3
SA014036MM66-4S2mutant VISA4
SA014037MM66-4S3mutant VISA4
SA014039MM66-4S1mutant VISA4
SA014044MM66-S3parent hVISA
SA014045MM66-S1parent hVISA
SA014046MM66-S2parent hVISA
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000323
Collection Summary:MM66 VISA selected on 3 mg/l vancomycin
Sample Type:Bacterial cells

Treatment:

Treatment ID:TR000343
Treatment Summary:MM66 VISA selected on 3 mg/l vancomycin

Sample Preparation:

Sampleprep ID:SP000337
Sampleprep Summary:The sample extraction was referred to the procedure of Roessner et al. (2000, 2001) with minor changes: About 10 mg (DW) of S. aureus cells were crashed in the mill (Model, Manufacturer), and then sonicated with the addition of 1.5 ml of 70% methanol for 10 min at 600C, followed by extraction with 1.5 ml water at room temperature. Both extracts were combined and dried in vacuum. Prior to GC/MS analysis, dried polar extracts were derivatized with 80 µl Methoxyamine hydrochloride (20mg ml-1) for 60 min at 400C and then with 80 µl MSTFA at 650C for 40 min.

Combined analysis:

Analysis ID AN000489
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column Agilent HP5-MS
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5973
Ion Mode POSITIVE
Units relative concentration/10mg DW

Chromatography:

Chromatography ID:CH000349
Instrument Name:Agilent 6890N
Column Name:Agilent HP5-MS
Flow Rate:1.3 ml min-1
Chromatography Type:GC

MS:

MS ID:MS000427
Analysis ID:AN000489
Instrument Name:Agilent 5973
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Ion Source Temperature:230C
Scanning Range:50-800 m/z
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