Summary of Study ST000384

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000301. The data can be accessed directly via it's Project DOI: 10.21228/M84P4J This work is supported by NIH grant, U2C- DK119886.


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Study IDST000384
Study TitleMetabolomic profiles in P. gingivalis cells treated with pABA
Study SummaryMany human infections are polymicrobial in origin, and synergistic interactions among community inhabitants control colonization and pathogenic potential (Murray et al., 2014). However, few interspecies interactions have been functionally dissected at the molecular level or characterized on a systems level. Periodontitis, which is the sixth most prevalent infectious disease worldwide (Kassebaum et al., 2014), is associated with a dysbiotic microbial community, and the keystone pathogen Porphyromonas gingivalis forms synergistic communities with the accessory pathogen Streptococcus gordonii (Lamont and Hajishengallis, 2015). P. gingivalis and S. gordonii communicate through co-adhesion and metabolite perception, and close association between P. gingivalis and S. gordonii results in significant changes in the expressed proteomes of both organisms (Kuboniwa et al., 2012, Hendrickson et al., 2012). Here we show that streptococcal 4 aminobenzoate/para-amino benzoic acid (pABA) is required for maximal accumulation of P. gingivalis in communities with S. gordonii. Exogenous pABA upregulates production of fimbrial interspecies adhesins and of a tyrosine phosphorylation-dependent signaling system in P. gingivalis. Consequently, fimbrial-dependent attachment and invasion of epithelial cells by P. gingivalis is also increased by pABA. Moreover, trans-omics studies performed by proteomics and metabolomics showed that pABA induces metabolic shifts within P. gingivalis, predominantly folate derivative biosynthesis. In a murine oral infection model, pABA increased colonization and survival of P. gingivalis, but did not increase virulence. The results establish streptococcal pABA as a major component of the interspecies S. gordonii-P. gingivalis interaction which regulates distinct aspects of polymicrobial synergy.
Osaka University
DepartmentGraduate School of Dentistry
Last NameKuboniwa
First NameMasae
Address1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan
Submit Date2016-04-16
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2016-04-16
Release Version1
Masae Kuboniwa Masae Kuboniwa application/zip

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Project ID:PR000301
Project DOI:doi: 10.21228/M84P4J
Project Title:Metabolomic profiles in P. gingivalis cells treated with pABA
Project Summary:Metabolomics data for Porphyromonas gingivalis in the presence or absence of 4 aminobenzoate/para-amino benzoic acid (pABA)
Institute:Osaka University
Last Name:Kuboniwa
First Name:Masae
Address:1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan


Subject ID:SU000405
Subject Type:Bacteria
Subject Species:Porphyromonas gingivalis ATCC 33277
Taxonomy ID:431947
Species Group:Microorganism


Subject type: Bacteria; Subject species: Porphyromonas gingivalis ATCC 33277 (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA017478pABA-3pABA (1 mg/ml)
SA017479pABA-1pABA (1 mg/ml)
SA017480pABA-2pABA (1 mg/ml)
Showing results 1 to 6 of 6


Collection ID:CO000399
Collection Summary:Cells were collected and washed with Milli-Q water by centrifugation.
Sample Type:Bacterial cells


Treatment ID:TR000419
Treatment Summary:P. gingivalis cells were incubated anaerobically with PBS in the presence or absence of 1 mg/ml of pABA at 37°C for 2 h.

Sample Preparation:

Sampleprep ID:SP000412
Sampleprep Summary:Bacterial pellets (5E+09 cfu) were immediately fixed by adding 5 µM internal standard-containing methanol.

Combined analysis:

Analysis ID AN000619
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent CE
Column Fused silica capillary 50um ×80 cm
MS instrument type TOF
MS instrument name Agilent CE-TOFMS
Units Relative Area


Chromatography ID:CH000444
Instrument Name:Agilent CE
Column Name:Fused silica capillary 50um ×80 cm
Chromatography Type:Reversed phase


MS ID:MS000552
Analysis ID:AN000619
Instrument Name:Agilent CE-TOFMS
Instrument Type:TOF