Summary of Study ST000384
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000301. The data can be accessed directly via it's Project DOI: 10.21228/M84P4J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000384 |
| Study Title | Metabolomic profiles in P. gingivalis cells treated with pABA |
| Study Summary | Many human infections are polymicrobial in origin, and synergistic interactions among community inhabitants control colonization and pathogenic potential (Murray et al., 2014). However, few interspecies interactions have been functionally dissected at the molecular level or characterized on a systems level. Periodontitis, which is the sixth most prevalent infectious disease worldwide (Kassebaum et al., 2014), is associated with a dysbiotic microbial community, and the keystone pathogen Porphyromonas gingivalis forms synergistic communities with the accessory pathogen Streptococcus gordonii (Lamont and Hajishengallis, 2015). P. gingivalis and S. gordonii communicate through co-adhesion and metabolite perception, and close association between P. gingivalis and S. gordonii results in significant changes in the expressed proteomes of both organisms (Kuboniwa et al., 2012, Hendrickson et al., 2012). Here we show that streptococcal 4 aminobenzoate/para-amino benzoic acid (pABA) is required for maximal accumulation of P. gingivalis in communities with S. gordonii. Exogenous pABA upregulates production of fimbrial interspecies adhesins and of a tyrosine phosphorylation-dependent signaling system in P. gingivalis. Consequently, fimbrial-dependent attachment and invasion of epithelial cells by P. gingivalis is also increased by pABA. Moreover, trans-omics studies performed by proteomics and metabolomics showed that pABA induces metabolic shifts within P. gingivalis, predominantly folate derivative biosynthesis. In a murine oral infection model, pABA increased colonization and survival of P. gingivalis, but did not increase virulence. The results establish streptococcal pABA as a major component of the interspecies S. gordonii-P. gingivalis interaction which regulates distinct aspects of polymicrobial synergy. |
| Institute | Osaka University |
| Department | Graduate School of Dentistry |
| Last Name | Kuboniwa |
| First Name | Masae |
| Address | 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan |
| kuboniwa@dent.osaka-u.ac.jp | |
| Phone | +81-6-6879-2922 |
| Submit Date | 2016-04-16 |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-04-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000301 |
| Project DOI: | doi: 10.21228/M84P4J |
| Project Title: | Metabolomic profiles in P. gingivalis cells treated with pABA |
| Project Summary: | Metabolomics data for Porphyromonas gingivalis in the presence or absence of 4 aminobenzoate/para-amino benzoic acid (pABA) |
| Institute: | Osaka University |
| Last Name: | Kuboniwa |
| First Name: | Masae |
| Address: | 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan |
| Email: | kuboniwa@dent.osaka-u.ac.jp |
| Phone: | +81-6-6879-2922 |
Subject:
| Subject ID: | SU000405 |
| Subject Type: | Bacteria |
| Subject Species: | Porphyromonas gingivalis ATCC 33277 |
| Taxonomy ID: | 431947 |
| Species Group: | Microorganism |
Factors:
Subject type: Bacteria; Subject species: Porphyromonas gingivalis ATCC 33277 (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA017475 | PBS-1 | - |
| SA017476 | PBS-3 | - |
| SA017477 | PBS-2 | - |
| SA017478 | pABA-3 | pABA (1 mg/ml) |
| SA017479 | pABA-1 | pABA (1 mg/ml) |
| SA017480 | pABA-2 | pABA (1 mg/ml) |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO000399 |
| Collection Summary: | Cells were collected and washed with Milli-Q water by centrifugation. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR000419 |
| Treatment Summary: | P. gingivalis cells were incubated anaerobically with PBS in the presence or absence of 1 mg/ml of pABA at 37°C for 2 h. |
Sample Preparation:
| Sampleprep ID: | SP000412 |
| Sampleprep Summary: | Bacterial pellets (5E+09 cfu) were immediately fixed by adding 5 µM internal standard-containing methanol. |
Combined analysis:
| Analysis ID | AN000619 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent CE |
| Column | Fused silica capillary 50um ×80 cm |
| MS Type | ESI |
| MS instrument type | TOF |
| MS instrument name | Agilent CE-TOFMS |
| Ion Mode | UNSPECIFIED |
| Units | Relative Area |
Chromatography:
| Chromatography ID: | CH000444 |
| Instrument Name: | Agilent CE |
| Column Name: | Fused silica capillary 50um ×80 cm |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000552 |
| Analysis ID: | AN000619 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| Ion Mode: | UNSPECIFIED |
