Summary of study ST000701

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000148. The data can be accessed directly via it's Project DOI: 10.21228/M8V011 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000701
Study TitleDifferences in metabolism in mouse embryonic fibroblasts (control vs SCF-beta-TrCP knockout)
Study TypeMS analysis
Study SummaryMouse embryonic fibroblasts were grown in DMED supplemented with 10%FBS. The same number of cells were plated in eight 6cm dishes. When the cells were ~70% confluent, four dishes received ethanol (carrier) and the other four received tamoxifen (1uM final) to induce knockout. The cells and media were harvested 4 days later. The media harvested from control and knockout dishes will be compared with the starting medium. The cells were washed with cold PBS twice while they are attached to the dishes. The whole dish with cells attached were immediately frozen in dry ice and wrapped with aluminum foil and stored at -80C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups6
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8V011
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000148
Project DOI:doi: 10.21228/M8V011
Project Title:trcp
Project Summary:metabolic phenotypes of a clock mutant mouse
Institute:Florida State University
Department:Biomedical Sciences
Laboratory:Lee Lab
Last Name:Lee
First Name:Choogon
Address:Tallahassee, FL
Email:choogon.lee@med.fsu.edu
Phone:850-645-1478

Subject:

Subject ID:SU000724
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id GENOTYPE Sample type
SA039526S00013520Control Fibroblasts
SA039527S00013519Control Fibroblasts
SA039528S00013521Control Fibroblasts
SA039529S00013518Control Fibroblasts
SA039530S00013515Control Fresh media
SA039531S00013516Control Spent media
SA039532S00013525Knockout Fibroblasts
SA039533S00013524Knockout Fibroblasts
SA039534S00013523Knockout Fibroblasts
SA039535S00013522Knockout Fibroblasts
SA039536S00013517Knockout Spent media
SA039537S00020125Pooled Fibroblasts
SA039538S00020124Pooled Spent media
Showing results 1 to 13 of 13

Collection:

Collection ID:CO000718
Collection Summary:-
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000738
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000731
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001089 AN001090
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000766
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000984
Analysis ID:AN001089
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000985
Analysis ID:AN001090
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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