Summary of study ST000834

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000595. The data can be accessed directly via it's Project DOI: 10.21228/M8RQ4G This work is supported by NIH grant, U2C- DK119886.

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Study IDST000834
Study TitlePurine and TCA measurements in salt sensitive (SS) hypertension
Study TypeMS analysis
Study SummarySS rats were surgically implanted with a chronic servo-control cuff, the purpose of which is to maintain normal pressure to the left kidney while the right kidney is exposed to high blood pressure. After 7 days of high salt treatment, which induces high blood pressure in the SS rat, rats were anesthetized with pentobarbital, kidneys were flushed and removed. The renal medulla was separated from the cortex using scissors, and the renal medullas were frozen in liquid nitrogen and stored at -80 C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups11
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailGC/LC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8RQ4G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000595
Project DOI:doi: 10.21228/M8RQ4G
Project Title:Purine and TCA Measurements in SS Hypertension
Project Type:MS analysis
Project Summary:Based on RNA-seq ontological and Bayesian analyses, both purine levels and TCA-cycle intermediates have been nominated as being regulated by pressure in the SS rat. This project seeks to verify the previous RNA-seq prediction.
Institute:Medical College of Wisconsin
Department:Physiology
Laboratory:Cowley Lab
Last Name:Cowley
First Name:Allen
Address:Milwaukee, WI
Email:cowley@mcw.edu
Phone:414-955-8277

Subject:

Subject ID:SU000910
Subject Type:RAT
Subject Species:Rattus
Taxonomy ID:10114
Species Group:Mammal

Factors:

Subject type: RAT; Subject species: Rattus (Factor headings shown in green)

mb_sample_id local_sample_id Side
SA050829S00025614Left
SA050830S00025608Left
SA050831S00025612Left
SA050832S00025610Left
SA050833S00025609Right
SA050834S00025615Right
SA050835S00025611Right
SA050836S00025613Right
Showing results 1 to 8 of 8

Collection:

Collection ID:CO000904
Collection Summary:-
Sample Type:Kidney

Treatment:

Treatment ID:TR000924
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000917
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001424 AN001425
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent Agilent 7890N
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent HP5-MS (15m × 0.25mm, 0.25 um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6520B QTOF Agilent 5975
Ion Mode NEGATIVE POSITIVE
Units counts/mg nmol/mg

Chromatography:

Chromatography ID:CH000995
Chromatography Summary:Amino, Aminopropyl HILIC
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH000996
Chromatography Summary:5% Phenyl Methyl Siloxane
Instrument Name:Agilent 7890N
Column Name:Agilent HP5-MS (15m × 0.25mm, 0.25 um)
Chromatography Type:GC

MS:

MS ID:MS001314
Analysis ID:AN001424
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001315
Analysis ID:AN001425
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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