Summary of study ST000886

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000615. The data can be accessed directly via it's Project DOI: 10.21228/M85Q46 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000886
Study TitleMechanism Behind Stay Green Trait in Bread Wheat (Triticum aestivum L.)
Study TypeComparison
Study SummaryTwo different wheat genotypes were treated with the high temperature and control conditions under full irrigated condition. Leaf tissues were collected for all 2-different treatments with six replicates after 7 and 10 days of high temperature treatment.
Institute
University of Florida
DepartmentAgronomy
Last NameBabar
First NameAli
Address3105 Mccarty Hall, gainesville, FL 32611
Emailmababar@ufl.edu
Phone217-552-2346
Submit Date2017-09-21
Num Groups8
Total Subjects48
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2018-10-10
Release Version1
Ali Babar Ali Babar
https://dx.doi.org/10.21228/M85Q46
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000615
Project DOI:doi: 10.21228/M85Q46
Project Title:World Food crops Breeding
Project Summary:The aim of the study is to perform LC-MS global metabolomics analysis on two different wheat genotypes were treated with the high temperature and control conditions under fully irrigated condition
Institute:University of Florida
Department:Agronomy
Last Name:Babar
First Name:Ali
Address:University of Florida
Email:mababar@ufl.edu
Phone:none

Subject:

Subject ID:SU000920
Subject Type:Plant
Subject Species:Triticum aestivum L.
Taxonomy ID:4565
Species Group:Plant

Factors:

Subject type: Plant; Subject species: Triticum aestivum L. (Factor headings shown in green)

mb_sample_id local_sample_id Strain Treatment Timepoint
SA05145720SONALIKA Control 10
SA05145821SONALIKA Control 10
SA05145924SONALIKA Control 10
SA05146019SONALIKA Control 10
SA05146123SONALIKA Control 10
SA05146222SONALIKA Control 10
SA05146310SONALIKA Control 7
SA05146412SONALIKA Control 7
SA05146511SONALIKA Control 7
SA0514669SONALIKA Control 7
SA0514677SONALIKA Control 7
SA0514688SONALIKA Control 7
SA05146927SONALIKA Heat Stress 10
SA05147028SONALIKA Heat Stress 10
SA05147130SONALIKA Heat Stress 10
SA05147229SONALIKA Heat Stress 10
SA05147326SONALIKA Heat Stress 10
SA05147425SONALIKA Heat Stress 10
SA05147514SONALIKA Heat Stress 7
SA05147615SONALIKA Heat Stress 7
SA05147717SONALIKA Heat Stress 7
SA05147816SONALIKA Heat Stress 7
SA05147918SONALIKA Heat Stress 7
SA05148013SONALIKA Heat Stress 7
SA05148151STAY GREEN Control 10
SA05148250STAY GREEN Control 10
SA05148352STAY GREEN Control 10
SA05148454STAY GREEN Control 10
SA05148549STAY GREEN Control 10
SA05148653STAY GREEN Control 10
SA05148737STAY GREEN Control 7
SA05148841STAY GREEN Control 7
SA05148939STAY GREEN Control 7
SA05149038STAY GREEN Control 7
SA05149142STAY GREEN Control 7
SA05149240STAY GREEN Control 7
SA05149357STAY GREEN Heat Stress 10
SA05149458STAY GREEN Heat Stress 10
SA05149559STAY GREEN Heat Stress 10
SA05149660STAY GREEN Heat Stress 10
SA05149756STAY GREEN Heat Stress 10
SA05149855STAY GREEN Heat Stress 10
SA05149944STAY GREEN Heat Stress 7
SA05150046STAY GREEN Heat Stress 7
SA05150148STAY GREEN Heat Stress 7
SA05150245STAY GREEN Heat Stress 7
SA05150347STAY GREEN Heat Stress 7
SA05150443STAY GREEN Heat Stress 7
Showing results 1 to 48 of 48

Collection:

Collection ID:CO000914
Collection Summary:None
Sample Type:Plant
Volumeoramount Collected:Flag leaf
Storage Conditions:-80 C

Treatment:

Treatment ID:TR000934
Treatment:Abiotic
Plant Growth Location:Gainesville, FL
Plant Light Period:12H
Plant Humidity:70%
Plant Temp:Control: 24C/12Hrs, 18C/12Hrs & Stress: Day 36C/12Hrs, Night 28C/12Hrs
Plant Watering Regime:Irrigated
Plant Estab Date:2016-09-09
Plant Harvest Date:2016-12-01
Plant Growth Stage:Anthesis
Plant Harvest Method:Leaves were washed with distilled water, cut and freeze in liquid nitrogen
Plant Storage:-80C

Sample Preparation:

Sampleprep ID:SP000927
Sampleprep Summary:Weigh 30mg of the lyophilized and pulverized sample into clean Eppendorf tube and record weight. Add 20μL of Metabolites Internal Standard Mix to each sample. Add 750 μL of Methanol and 750 μL of 10mM Ammonium Acetate to each sample. Homogenize for 30 seconds at 1800 rpm on the BeadBeater. Sonicate for 10-20 minutes at room temperature. Centrifuge at room temperature for 10 minutes at 17,000 xg. Transfer 50 μL of supernatant to a labeled LC vial with a fused insert. Transfer the rest of the supernatant to a labeled 2 mL LC Vial with no fused insert to store in -80°C.
Sampleprep Protocol Filename:Plant_Tissue_SamplePrep_Instrument_method.pdf
Appendix_A -_Internal_Standard_Prep_GLCMS.pdf

Combined analysis:

Analysis ID AN001443 AN001444
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak Height Peak Height

Chromatography:

Chromatography ID:CH001014
Methods Filename:Plant_Tissue_SamplePrep_Instrument_method.pdf
Chromatography Comments:Reverse phase or HILIC- HPLC-MS
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:350ul/min
Solvent A:0.1% Formic Acid in Water
Solvent B:Acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS001333
Analysis ID:AN001443
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001334
Analysis ID:AN001444
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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