Summary of study ST001065

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000713. The data can be accessed directly via it's Project DOI: 10.21228/M8HM48 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001065
Study TitleLipidomics analysis for aged mice brain cortex (part-II)
Study TypeMS lipidomics analysis
Study SummaryLipidomics studies on samples from aged and young mice.
Institute
Takeda Pharmaceutical Company Limited
Last NameAndo
First NameAyumi
Address26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa 251-8555, Japan
Emailayumi.kawamura@takeda.com
Phone+81466-32-4216
Submit Date2018-09-30
Raw Data AvailableYes
Raw Data File Type(s).gda
Analysis Type DetailLC-MS
Release Date2019-10-11
Release Version1
Ayumi Ando Ayumi Ando
https://dx.doi.org/10.21228/M8HM48
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000713
Project DOI:doi: 10.21228/M8HM48
Project Title:Lipidomics analysis for aged mice organs
Project Type:MS lipidomics analysis
Project Summary:Lipidomics analysis for aged mice organs
Institute:Takeda Pharmaceutical Company Limited
Last Name:Ando
First Name:Ayumi
Address:26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa, 251-8555, Japan
Email:ayumi.kawamura@takeda.com
Phone:+81-466-32-4216

Subject:

Subject ID:SU001109
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J mice
Age Or Age Range:8 week (“young”) and 112 week (“old”).
Weight Or Weight Range:young: 20.2g (mean), aged: 34.3g (mean)
Gender:Male
Animal Animal Supplier:CLEA (Tokyo, Japan)
Animal Light Cycle:a 12-hour light/12-hour dark schedule
Animal Feed:CE-2, CLEA
Animal Water:tap water

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Age Tissue
SA071942Cortex_60_agedAged 112 weeks Cerebral Cortex
SA071943Cortex_58_agedAged 112 weeks Cerebral Cortex
SA071944Cortex_56_agedAged 112 weeks Cerebral Cortex
SA071945Cortex_54_agedAged 112 weeks Cerebral Cortex
SA071946Cortex_52_agedAged 112 weeks Cerebral Cortex
SA071947Adipose_60_agedAged 112 weeks Epididymal adipose tissue
SA071948Adipose_58_agedAged 112 weeks Epididymal adipose tissue
SA071949Adipose_52_agedAged 112 weeks Epididymal adipose tissue
SA071950Adipose_54_agedAged 112 weeks Epididymal adipose tissue
SA071951Adipose_56_agedAged 112 weeks Epididymal adipose tissue
SA071952Femoral_52_agedAged 112 weeks Femoral muscle
SA071953Femoral_58_agedAged 112 weeks Femoral muscle
SA071954Femoral_60_agedAged 112 weeks Femoral muscle
SA071955Femoral_56_agedAged 112 weeks Femoral muscle
SA071956Femoral_54_agedAged 112 weeks Femoral muscle
SA071957Liver_60_agedAged 112 weeks Liver
SA071958Liver_58_agedAged 112 weeks Liver
SA071959Liver_56_agedAged 112 weeks Liver
SA071960Liver_52_agedAged 112 weeks Liver
SA071961Liver_54_agedAged 112 weeks Liver
SA071962Cortex_59_youngYoung 8 weeks Cerebral Cortex
SA071963Cortex_57_youngYoung 8 weeks Cerebral Cortex
SA071964Cortex_51_youngYoung 8 weeks Cerebral Cortex
SA071965Cortex_55_youngYoung 8 weeks Cerebral Cortex
SA071966Cortex_53_youngYoung 8 weeks Cerebral Cortex
SA071967Adipose_59_youngYoung 8 weeks Epididymal adipose tissue
SA071968Adipose_57_youngYoung 8 weeks Epididymal adipose tissue
SA071969Adipose_55_youngYoung 8 weeks Epididymal adipose tissue
SA071970Adipose_51_youngYoung 8 weeks Epididymal adipose tissue
SA071971Adipose_53_youngYoung 8 weeks Epididymal adipose tissue
SA071972Femoral_57_youngYoung 8 weeks Femoral muscle
SA071973Femoral_59_youngYoung 8 weeks Femoral muscle
SA071974Femoral_55_youngYoung 8 weeks Femoral muscle
SA071975Femoral_53_youngYoung 8 weeks Femoral muscle
SA071976Femoral_51_youngYoung 8 weeks Femoral muscle
SA071977Liver_59_youngYoung 8 weeks Liver
SA071978Liver_57_youngYoung 8 weeks Liver
SA071979Liver_55_youngYoung 8 weeks Liver
SA071980Liver_53_youngYoung 8 weeks Liver
SA071981Liver_51_youngYoung 8 weeks Liver
Showing results 1 to 40 of 40

Collection:

Collection ID:CO001103
Collection Summary:All mice were killed by decapitation for sample collection. Collected tissues were immediately frozen by liquid nitrogen then stored -80 C until use. (Femoral, Cortex, Liver, Adipose) tissues.
Sample Type:Brain
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001123
Treatment Summary:Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics.

Sample Preparation:

Sampleprep ID:SP001116
Sampleprep Summary:Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics.

Combined analysis:

Analysis ID AN001742 AN001743
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Orbitrap XL Orbitrap XL
Column Waters XBridge C18 (50 x 2.1mm, 3um) Waters XBridge C18 (50 x 2.1mm, 3um)
MS Type ESI ESI
MS instrument type Single quadrupole Single quadrupole
MS instrument name Thermo LTQ XL Thermo LTQ XL
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH001231
Chromatography Summary:The liquid chromatography/mass spectrometry system consisted of an Ultimate3000 RSLC system (Thermo Fisher Scientific inc., Sunnyvale, CA, USA) using a gradient elution consisting of mobile phase A (0.01% acetic acid and 1 mM ammonia in MilliQ water) and mobile phase B (0.001% acetic acid and 0.2 mM ammonia in ethanol/isopropanol [3:1])
Instrument Name:Orbitrap XL
Column Name:Waters XBridge C18 (50 x 2.1mm, 3um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001610
Analysis ID:AN001742
Instrument Name:Thermo LTQ XL
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001611
Analysis ID:AN001743
Instrument Name:Thermo LTQ XL
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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