Summary of study ST001101

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000737. The data can be accessed directly via it's Project DOI: 10.21228/M8DM5Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST001101
Study TitleIdentification of potential sepsis biomarkers in burn injury conditions.
Study Summary12 mice were subjected to one of four treatment groups: no burn injury:no injection, burn injury:no infection, no burn injury:infection, and burn injury:infection. The researchers in this study are looking for metabolites in serum that could indicate sepsis in mice with burn injuries.
Institute
Texas Tech University
DepartmentDepartment of Biological Sciences
Last NameElmassry
First NameMoamen
Address 2901 Main St, Lubbock, TX 79409
Emailmoamen.elmassry@ttu.edu
Phone806-742-2715
Submit Date2018-11-29
Num Groups4
Total Subjects12
Raw Data AvailableYes
Analysis Type DetailGC-MS
Release Date2019-01-22
Release Version1
Moamen Elmassry Moamen Elmassry
https://dx.doi.org/10.21228/M8DM5Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000737
Project DOI:doi: 10.21228/M8DM5Q
Project Title:Identifying sepsis biomarkes, using GC-MS, in mice with burn injuries
Project Summary:12 mice were subjected to one of four treatment groups. After completion of treatment, mouse serum was taken from each mouse, prepared for analysis, and they studied using GC-MS analysis.
Institute:University of California, Davis
Department:Genome and Biomedical Sciences Facility
Laboratory:WCMC Metabolomics Core
Last Name:Fiehn
First Name:Oliver
Address:1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Email:ofiehn@ucdavis.edu
Phone:(530) 754-8258

Subject:

Subject ID:SU001145
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Burn Status Infected
SA075053FBurned No
SA075054DBurned No
SA075055EBurned No
SA075056KBurned Yes
SA075057JBurned Yes
SA075058LBurned Yes
SA075059AHealthy No
SA075060CHealthy No
SA075061BHealthy No
SA075062GHealthy Yes
SA075063HHealthy Yes
SA075064IHealthy Yes
Showing results 1 to 12 of 12

Collection:

Collection ID:CO001139
Collection Summary:Blood were aseptically collected from mice directly from the heart then left at room temperature for a few hours to clot, then centrifuged to separate the serum. Serum was then packed on dry ice and sent to the WCMC center in Davis, CA.
Sample Type:Serum

Treatment:

Treatment ID:TR001159
Treatment Summary:Mice were placed into 1 of 4 treatment groups, with three mice in each group. The first group contained uninjured mice who were not infected. The second group contained thermally injured mice who were not infected. The third group contained mice that were uninjured and infected with Pseudomonas aeruginosa. The fourth group of mice were thermally injured and infected with Pseudomonas aeruginosa.

Sample Preparation:

Sampleprep ID:SP001152
Sampleprep Summary:30µL of plasma was aliquoted into a 1.5 mL eppendorph tube. 1 mL of 3:3:2 ACN:H20:IPA was added to each sample. Samples were vortexed for 10 seconds and then shaken at 4°C for 5 minutes using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 x 475 µL aliquots were removed, one dried down and submitted to derivitization, the other frozen at -20°C for backup. Samples were derivatized by adding 10 µL of MeOX and then shaken at 30°C for 1.5 hours using a orbital mixing plate. 91 µL of MSTFA + FAMES mixutre is added to each sample. Samples were shaken at 37°C for 30 minutes. Samples submitted to GC analysis.

Combined analysis:

Analysis ID AN001790
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus III GC TOF
Ion Mode POSITIVE
Units Peak Height

Chromatography:

Chromatography ID:CH001264
Instrument Name:Agilent 7890A
Column Name:Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
Column Temperature:50-330°C
Flow Rate:1 mL min^(-1)
Injection Temperature:50°C ramped to 250°C by 12°C s-1
Oven Temperature:50°C for 1 min, then ramped at 20°C min-1 to 330°C, held constant for 5 min
Chromatography Type:GC

MS:

MS ID:MS001652
Analysis ID:AN001790
Instrument Name:Leco Pegasus III GC TOF
Instrument Type:GC-TOF
MS Type:EI
Ion Mode:POSITIVE
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