Summary of study ST001113

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000744. The data can be accessed directly via it's Project DOI: 10.21228/M8HD5K This work is supported by NIH grant, U2C- DK119886.

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Study IDST001113
Study TitleExposure to Oral Contraceptives Alters Human Endometrial Stem Cells Culture Media Metabolomics
Study SummaryAlthough the effects of oral contraceptives (OCs) in the endometrium has been well established, its influence in the production and metabolism of endometrial mesenchymal stem cells (EnMSC) remains unclear. Therefore, we analyzed the effect of OCs in the EnMSC secretome by culture media quantitative metabolomics. The EnMSC were collected from menstrual shedding of five donors (OC group, n=3; control, non-OC group, n=2) and cultured for three passages. Cells characterization was performed by flow cytometry, and culture media was collected at the end of each passage for further quantitative metabolomics. The metabolites with higher discriminant power for sample classification were Alanine, PC aa C30:0, c4-OH-Pro, PC aa C32:2, PC ae C32:2, PC ae C40:2, glycine and PC ae C32:1 for the OC group, whereas PC aa C36:6, PC aa C34:4, SM OH C16:1, SM C26:0, PC aa C38:0, serine and PC aa C36:5 were representative of the non-OC group. This panel of metabolites showed 98% of sensitivity in sample classification according with respective groups. Altered concentrations of metabolites may be an effect of OC hormonal properties on EnMSC metabolism. Thus, this metabolomic approach could assist in the management of future stem cell therapies according to patients’ specific responses to hormone treatments.
Institute
Universidade Federal de Sao Paulo
Last NameCordeiro
First NameFernanda
AddressRua Embau, 231, Vila Clementino
Emailfernandabertuccez85@gmail.com
Phone11996667402
Submit Date2018-01-16
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Fernanda Cordeiro Fernanda Cordeiro
https://dx.doi.org/10.21228/M8HD5K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000744
Project DOI:doi: 10.21228/M8HD5K
Project Title:Exposure to Oral Contraceptives Alters Human Endometrial Stem Cells Culture Media Metabolomics
Project Summary:The project analyzed the effect of oral contraceptives in the metabolism of endometrial mesenchmal stem cells, via culture media quantitative metabolomics.
Institute:Universidade Federal de Sao Paulo
Last Name:Bertuccez Cordeiro
First Name:Fernanda
Address:Rua Embau, 231, Vila Clementino, Sao Paulo, São Paulo, 04039-060, Brazil
Email:fernandabertuccez85@gmail.com
Phone:5511996667402

Subject:

Subject ID:SU001158
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Woman

Factors:

Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Study Group
SA075910R12non-OC
SA075911R8non-OC
SA075912R7non-OC
SA075913R9non-OC
SA075914R10non-OC
SA075915R11non-OC
SA075901R1OC
SA075902R15OC
SA075903R14OC
SA075904R13OC
SA075905R6OC
SA075906R2OC
SA075907R3OC
SA075908R4OC
SA075909R5OC
Showing results 1 to 15 of 15

Collection:

Collection ID:CO001152
Collection Summary:The endometrial mesenchymal stem cells (EnMSC) were collected from menstrual shedding, isolated and cultured in vitro from donors who used or not oral contraceptives. The EnMSC were grown up until the second passage (P2) and 24h before the end of each passage (P0, P1 and P2) the culture medium was completely replaced for the collection. The samples were centrifuged at 400 × g for 5 minutes, filtered with 0,2 µm filter and stored at -80 ºC until the metabolomics analysis without any extraction method.
Sample Type:Stem cells culture media

Treatment:

Treatment ID:TR001172
Treatment Summary:Culture media samples were collected by the end of each passage, kept under -20C until the moment of analysis.

Sample Preparation:

Sampleprep ID:SP001165
Sampleprep Summary:The endometrial mesenchymal stem cells (EnMSC) were collected from menstrual shedding, isolated and cultured in vitro from donors who used or not oral contraceptives. The EnMSC were grown up until the second passage (P2) and 24h before the end of each passage (P0, P1 and P2) the culture medium was completely replaced for the collection. The samples were centrifuged at 400 × g for 5 minutes, filtered with 0,2 µm filter and stored at -80 ºC until the metabolomics analysis without any extraction method.

Combined analysis:

Analysis ID AN001809
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system None
Column none
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 4000 QTrap
Ion Mode UNSPECIFIED
Units umol

Chromatography:

Chromatography ID:CH001274
Instrument Name:None
Column Name:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS001667
Analysis ID:AN001809
Instrument Name:ABI Sciex 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:UNSPECIFIED
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