Summary of Study ST001300
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000881. The data can be accessed directly via it's Project DOI: 10.21228/M8T38D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001300 |
| Study Title | Luminal metabolome profiles ofUC-HMA mice transplanted with a healthy human-derived fecal microbiota (FMT). |
| Study Summary | Luminal (fecal) metabolome of FMT treated UC-HMA mice and FMT untreated UC-HMA mice were compared. |
| Institute | University of Michigan |
| Department | Internal Medicine |
| Last Name | Kamada |
| First Name | Nobuhiko |
| Address | 1150 W Medical center Dr, Ann Arbor, MI 48109, USA |
| nkamada@umich.edu | |
| Phone | +1-734-763-2142 |
| Submit Date | 2019-12-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-01-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000881 |
| Project DOI: | doi: 10.21228/M8T38D |
| Project Title: | Luminal metabolome profiles ofUC-HMA mice transplanted with a healthy human-derived fecal microbiota (FMT). |
| Project Summary: | We found that UC-HMA mice, GF mice were colonized with gut microbiota derived from patients with UC, were susceptible to Clostridioides difficile (C. difficile) infection. However, FMT almost completely prevented the growth of C. difficile in the gut of UC-HMA mice. To clear this mechanism, fecal samples were collected from FMT-treated and -untreated UC-HMA mice, and metabolite profiles of them were analyzed. |
| Institute: | University of Michigan |
| Department: | Internal Medicine |
| Last Name: | Kamada |
| First Name: | Nobuhiko |
| Address: | 1150 W Medical center Dr, Ann Arbor, MI 48109, USA |
| Email: | nkamada@umich.edu |
| Phone: | +1-734-763-2142 |
Subject:
| Subject ID: | SU001374 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA094241 | FMT8 | Control |
| SA094242 | FMT9 | Control |
| SA094243 | FMT1 | Control |
| SA094244 | FMT6 | Control |
| SA094245 | FMT7 | Control |
| SA094246 | FMT2 | Control |
| SA094247 | FMT4 | Control |
| SA094248 | FMT3 | Control |
| SA094249 | FMT5 | Control |
| SA094250 | FMT16 | FMT |
| SA094251 | FMT17 | FMT |
| SA094252 | FMT18 | FMT |
| SA094253 | FMT15 | FMT |
| SA094254 | FMT10 | FMT |
| SA094255 | FMT11 | FMT |
| SA094256 | FMT12 | FMT |
| SA094257 | FMT13 | FMT |
| SA094258 | FMT14 | FMT |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO001369 |
| Collection Summary: | Feces of FMT-treated/-untreated UC-HMA mice were harvested. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR001389 |
| Treatment Summary: | GF C57BL/6 mice were colonized with UC microbiotas for 2 weeks. After colonization of HC microbiota, the mice were transplanted with healthy human microbiota once (day -3) before collecting fecal samples. |
Sample Preparation:
| Sampleprep ID: | SP001382 |
| Sampleprep Summary: | Fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol. |
Combined analysis:
| Analysis ID | AN002164 | AN002165 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent 7100 CE | Agilent 7100 CE |
| Column | Fused-silica,50um(cation) | COSMO(+),50um(anion |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Agilent G3250AA LC/MSD TOF system | Agilent G3250AA LC/MSD TOF system |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | nmol/g | nmol/g |
Chromatography:
| Chromatography ID: | CH001582 |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Fused-silica,50um(cation) |
| Internal Standard: | 20 μM of methionine sulfone |
| Chromatography Type: | CE |
| Chromatography ID: | CH001583 |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | COSMO(+),50um(anion |
| Internal Standard: | 20 μM of D-camphor-10-sulfonic acid (CSA) |
| Chromatography Type: | CE |
MS:
| MS ID: | MS002013 |
| Analysis ID: | AN002164 |
| Instrument Name: | Agilent G3250AA LC/MSD TOF system |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002014 |
| Analysis ID: | AN002165 |
| Instrument Name: | Agilent G3250AA LC/MSD TOF system |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | NEGATIVE |
