Summary of study ST001300

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000881. The data can be accessed directly via it's Project DOI: 10.21228/M8T38D This work is supported by NIH grant, U2C- DK119886.

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Study IDST001300
Study TitleLuminal metabolome profiles ofUC-HMA mice transplanted with a healthy human-derived fecal microbiota (FMT).
Study SummaryLuminal (fecal) metabolome of FMT treated UC-HMA mice and FMT untreated UC-HMA mice were compared.
Institute
University of Michigan
DepartmentInternal Medicine
Last NameKamada
First NameNobuhiko
Address1150 W Medical center Dr, Ann Arbor, MI 48109, USA
Emailnkamada@umich.edu
Phone+1-734-763-2142
Submit Date2019-12-24
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2020-01-08
Release Version1
Nobuhiko Kamada Nobuhiko Kamada
https://dx.doi.org/10.21228/M8T38D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000881
Project DOI:doi: 10.21228/M8T38D
Project Title:Luminal metabolome profiles ofUC-HMA mice transplanted with a healthy human-derived fecal microbiota (FMT).
Project Summary:We found that UC-HMA mice, GF mice were colonized with gut microbiota derived from patients with UC, were susceptible to Clostridioides difficile (C. difficile) infection. However, FMT almost completely prevented the growth of C. difficile in the gut of UC-HMA mice. To clear this mechanism, fecal samples were collected from FMT-treated and -untreated UC-HMA mice, and metabolite profiles of them were analyzed.
Institute:University of Michigan
Department:Internal Medicine
Last Name:Kamada
First Name:Nobuhiko
Address:1150 W Medical center Dr, Ann Arbor, MI 48109, USA
Email:nkamada@umich.edu
Phone:+1-734-763-2142

Subject:

Subject ID:SU001374
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA094241FMT8Control
SA094242FMT9Control
SA094243FMT1Control
SA094244FMT6Control
SA094245FMT7Control
SA094246FMT2Control
SA094247FMT4Control
SA094248FMT3Control
SA094249FMT5Control
SA094250FMT16FMT
SA094251FMT17FMT
SA094252FMT18FMT
SA094253FMT15FMT
SA094254FMT10FMT
SA094255FMT11FMT
SA094256FMT12FMT
SA094257FMT13FMT
SA094258FMT14FMT
Showing results 1 to 18 of 18

Collection:

Collection ID:CO001369
Collection Summary:Feces of FMT-treated/-untreated UC-HMA mice were harvested.
Sample Type:Feces

Treatment:

Treatment ID:TR001389
Treatment Summary:GF C57BL/6 mice were colonized with UC microbiotas for 2 weeks. After colonization of HC microbiota, the mice were transplanted with healthy human microbiota once (day -3) before collecting fecal samples.

Sample Preparation:

Sampleprep ID:SP001382
Sampleprep Summary:Fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol.

Combined analysis:

Analysis ID AN002164 AN002165
Analysis type MS MS
Chromatography type Other Other
Chromatography system Agilent 7100 CE Agilent 7100 CE
Column Fused-silica, i.d. 50 µm(cation) COSMO(+), i.d.50 μm(anion
MS Type ESI ESI
MS instrument type Other Other
MS instrument name Agilent G3250AA LC/MSD TOF system Agilent G3250AA LC/MSD TOF system
Ion Mode POSITIVE NEGATIVE
Units nmol/g nmol/g

Chromatography:

Chromatography ID:CH001582
Instrument Name:Agilent 7100 CE
Column Name:Fused-silica, i.d. 50 µm(cation)
Internal Standard:20 μM of methionine sulfone
Chromatography Type:Other
  
Chromatography ID:CH001583
Instrument Name:Agilent 7100 CE
Column Name:COSMO(+), i.d.50 μm(anion
Internal Standard:20 μM of D-camphor-10-sulfonic acid (CSA)
Chromatography Type:Other

MS:

MS ID:MS002013
Analysis ID:AN002164
Instrument Name:Agilent G3250AA LC/MSD TOF system
Instrument Type:Other
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:POSITIVE
  
MS ID:MS002014
Analysis ID:AN002165
Instrument Name:Agilent G3250AA LC/MSD TOF system
Instrument Type:Other
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:NEGATIVE
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