Summary of Study ST001350

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000920. The data can be accessed directly via it's Project DOI: 10.21228/M8S11R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001350
Study TitleExtraction of high-resolution Metabolomics data of the Yeast Metabolic Cycle
Study SummaryExtraction of a high resolution metabolomics dataset through the Yeast Metabolic Cycle to complete a multi-omics dataset in this model system
Institute
University of Florida
Last NameConesa
First NameAna
Address2033 Mowry Rd, Gainesville, FL 32610-3633
Emailsalvacasani@gmail.com / aconesa@ufl.edu
Phone352-273-8127
Submit Date2020-03-06
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2020-12-01
Release Version1
Ana Conesa Ana Conesa
https://dx.doi.org/10.21228/M8S11R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000920
Project DOI:doi: 10.21228/M8S11R
Project Title:Development of multiomics methods for the integrative study of the Yeast Metabolic Cycle
Project Summary:Extraction of metabolomics, epigenetics and gene expression datasets in the Yeast Metabolic Cycle for the development of statistical methods for omics integration
Institute:University of Florida
Last Name:Conesa
First Name:Ana
Address:2033 Mowry Rd, Gainesville, FL 32610-3633
Email:salvacasani@gmail.com / aconesa@ufl.edu
Phone:352-273-8127

Subject:

Subject ID:SU001424
Subject Type:Yeast
Subject Species:Saccharomyces cerevisiae
Taxonomy ID:4932
Species Group:Yeast

Factors:

Subject type: Yeast; Subject species: Saccharomyces cerevisiae (Factor headings shown in green)

mb_sample_id local_sample_id Timepoint Replicate
SA097980Sample 1010 1
SA097981Sample 3110 2
SA097982Sample 4910 3
SA097983Sample 7310 4
SA097984Sample 9510 5
SA097985Sample 1111 1
SA097986Sample 3211 2
SA097987Sample 5011 3
SA097988Sample 7411 4
SA097989Sample 9611 5
SA097990Sample 1212 1
SA097991Sample 3312 2
SA097992Sample 5112 3
SA097993Sample 7512 4
SA097994Sample 9712 5
SA097995Sample 1313 1
SA097996Sample 3413 2
SA097997Sample 5213 3
SA097998Sample 7613 4
SA097999Sample 9813 5
SA098000Sample 1414 1
SA098001Sample 3514 2
SA098002Sample 5314 3
SA098003Sample 7714 4
SA098004Sample 9914 5
SA098005Sample 1515 1
SA098006Sample 3615 2
SA098007Sample 5415 3
SA098008Sample 7815 4
SA098009Sample 10015 5
SA098010Sample 1616 1
SA098011Sample 3716 2
SA098012Sample 5516 3
SA098013Sample 7916 4
SA098014Sample 10116 5
SA098015Sample 1717 1
SA098016Sample 3817 2
SA098017Sample 5617 3
SA098018Sample 8017 4
SA098019Sample 10217 5
SA098020Sample 1818 1
SA098021Sample 3918 2
SA098022Sample 5718 3
SA098023Sample 8118 4
SA098024Sample 10318 5
SA098025Sample 1919 1
SA098026Sample 4019 2
SA098027Sample 5819 3
SA098028Sample 8219 4
SA098029Sample 10419 5
SA098030Sample 11 1
SA098031Sample 221 2
SA098032Sample 611 3
SA098033Sample 641 4
SA098034Sample 861 5
SA098035Sample 2020 1
SA098036Sample 4120 2
SA098037Sample 5920 3
SA098038Sample 8320 4
SA098039Sample 10520 5
SA098040Sample 2121 1
SA098041Sample 4221 2
SA098042Sample 6021 3
SA098043Sample 8421 4
SA098044Sample 10621 5
SA098045Sample 22 1
SA098046Sample 232 2
SA098047Sample 622 3
SA098048Sample 652 4
SA098049Sample 872 5
SA098050Sample 33 1
SA098051Sample 243 2
SA098052Sample 633 3
SA098053Sample 663 4
SA098054Sample 883 5
SA098055Sample 44 1
SA098056Sample 254 2
SA098057Sample 434 3
SA098058Sample 674 4
SA098059Sample 894 5
SA098060Sample 55 1
SA098061Sample 265 2
SA098062Sample 445 3
SA098063Sample 685 4
SA098064Sample 905 5
SA098065Sample 66 1
SA098066Sample 276 2
SA098067Sample 456 3
SA098068Sample 696 4
SA098069Sample 916 5
SA098070Sample 77 1
SA098071Sample 287 2
SA098072Sample 467 3
SA098073Sample 707 4
SA098074Sample 927 5
SA098075Sample 88 1
SA098076Sample 298 2
SA098077Sample 478 3
SA098078Sample 718 4
SA098079Sample 938 5
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Collection:

Collection ID:CO001419
Collection Summary:Samples were collected from fermentor eppendorf bioflo 120 through different timepoints of the Yeast Metabolic Cycle. Cells were snap frozen on liquid nitrogen for metabolite extraction.
Sample Type:Yeast cells

Treatment:

Treatment ID:TR001439
Treatment Summary:Cells were not treated, this was a timecourse on stable conditions, that yeast cells use to enter an ultradian rythm, which was measured during the timecourse.

Sample Preparation:

Sampleprep ID:SP001432
Sampleprep Summary:Cell pellets were lyophilized to dryness overnight. The lyophilized pellet was homogenized in 50:50 acetonitrile/water with 0.3 % formic acid for OAs and AAs, 5% TCA for acetyl and malonyl CoA, 0.5M PCA for oxidized nucleotides and 50:50 methanol/NaOH for reduced nucleotides using the Precellys bead-based homogenizing system. Precellys tough micro-organism lysing kit (VK-05) was used to burst the yeast cells. An aliquot of homogenate for the required assay was aliquoted and immediately stored at -80oC.

Chromatography:

Chromatography ID:CH001651
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Hypercarb
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN002246
Analysis Type:MS
Chromatography ID:CH001651
Num Factors:107
Num Metabolites:63
Units:nmoles/mg protein
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