Summary of Study ST001668

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001071. The data can be accessed directly via it's Project DOI: 10.21228/M8870S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001668
Study Title	D-Allulose effects on hepatic metabolomics profile in rodents
Study Summary	To determine the hepatic metabolomics profile after D-allulose intake.
Institute	Matsutani Chemical Industry Co., Ltd.
Last Name	Kanasaki
First Name	Akane
Address	5-3 Kita-Itami, Itami, Hyogo, 664-8508, Japan
Email	akane-kanasaki@matsutani.co.jp
Phone	81-72-771-2052
Submit Date	2021-01-19
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2022-01-19
Release Version	1

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Project:

Project ID:	PR001071
Project DOI:	doi: 10.21228/M8870S
Project Title:	D-Allulose effects on metabolomics profile in rodents
Project Summary:	To determine the metabolomics profile after D-allulose intake.
Institute:	Matsutani Chemical Industry Co., Ltd.
Last Name:	Kanasaki
First Name:	Akane
Address:	5-3 Kita-Itami, Itami, Hyogo, 664-8508, Japan
Email:	akane-kanasaki@matsutani.co.jp
Phone:	81-72-771-2052

Subject:

Subject ID:	SU001745
Subject Type:	Mammal
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116
Species Group:	Mammals

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Diet
SA152357	C3	control
SA152358	C2	control
SA152359	C4	control
SA152360	C5	control
SA152361	C6	control
SA152362	C1	control
SA152351	P3	D-Allulose
SA152352	P4	D-Allulose
SA152353	P6	D-Allulose
SA152354	P2	D-Allulose
SA152355	P5	D-Allulose
SA152356	P1	D-Allulose

Showing results 1 to 12 of 12

Showing results 1 to 12 of 12

Collection:

Collection ID:	CO001738
Collection Summary:	The livers were collected and then flash-frozen in liquid N2
Sample Type:	Liver

Treatment:

Treatment ID:	TR001758
Treatment Summary:	Seven-week old male Wistar rats were fed AIN-93G-based diets with/without 3% D-allulose, ad libitum for 4 weeks.

Sample Preparation:

Sampleprep ID:	SP001751
Sampleprep Summary:	For CE-TOFMS, The frozen tissue was plunged into 50% acetonitrile/Milli-Q water containing internal standards. The tissues were homogenized thrice, and then centrifuged. The upper aqueous layer was centrifugally filtered to remove the proteins. The filtrate was then centrifugally concentrated and re-suspended in 50 µL of Milli-Q water for CE-MS analysis. For LC-TOFMS, the frozen tissue was plunged into 1% formic acid/acetonitrile containing the internal standard solution. Tissue was homogenized thrice, and the mixture was yet again homogenized after adding Milli-Q water and then centrifuged. The supernatant was collected and 500 1% formic acid/acetonitrile and Milli-Q water were added to the precipitate. Homogenization and centrifugation were performed as described previously, and the supernatant was mixed with previously collected one. The mixed supernatant was filtrated to remove the proteins and phospholipids. The filtrate was then desiccated and dissolved in 200 µL of isopropanol/Milli-Q water for LC-MS analysis.

Sampleprep ID:

SP001751

Sampleprep Summary:

For CE-TOFMS, The frozen tissue was plunged into 50% acetonitrile/Milli-Q water containing internal standards. The tissues were homogenized thrice, and then centrifuged. The upper aqueous layer was centrifugally filtered to remove the proteins. The filtrate was then centrifugally concentrated and re-suspended in 50 µL of Milli-Q water for CE-MS analysis. For LC-TOFMS, the frozen tissue was plunged into 1% formic acid/acetonitrile containing the internal standard solution. Tissue was homogenized thrice, and the mixture was yet again homogenized after adding Milli-Q water and then centrifuged. The supernatant was collected and 500 1% formic acid/acetonitrile and Milli-Q water were added to the precipitate. Homogenization and centrifugation were performed as described previously, and the supernatant was mixed with previously collected one. The mixed supernatant was filtrated to remove the proteins and phospholipids. The filtrate was then desiccated and dissolved in 200 µL of isopropanol/Milli-Q water for LC-MS analysis.

Chromatography:

Chromatography ID:	CH002008
Instrument Name:	Agilent CE
Column Name:	fused silica capillary (50 μm i.d. × 80 cm)
Chromatography Type:	CE

Chromatography ID:	CH002009
Instrument Name:	Agilent 1200SL
Column Name:	ODS (2x50mm,2um)
Chromatography Type:	Reversed phase

Analysis:

Analysis ID:	AN002720
Analysis Type:	MS
Chromatography ID:	CH002008
Num Factors:	2
Num Metabolites:	137
Units:	Relative Area

Analysis ID:	AN002721
Analysis Type:	MS
Chromatography ID:	CH002008
Num Factors:	2
Num Metabolites:	133
Units:	Relative Area

Analysis ID:	AN002722
Analysis Type:	MS
Chromatography ID:	CH002009
Num Factors:	2
Num Metabolites:	37
Units:	Relative Area

Analysis ID:	AN002723
Analysis Type:	MS
Chromatography ID:	CH002009
Num Factors:	2
Num Metabolites:	44
Units:	Relative Area