Summary of Study ST001668

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001071. The data can be accessed directly via it's Project DOI: 10.21228/M8870S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST001668
Study TitleD-Allulose effects on hepatic metabolomics profile in rodents
Study SummaryTo determine the hepatic metabolomics profile after D-allulose intake.
Institute
Matsutani Chemical Industry Co., Ltd.
Last NameKanasaki
First NameAkane
Address5-3 Kita-Itami, Itami, Hyogo, 664-8508, Japan
Emailakane-kanasaki@matsutani.co.jp
Phone81-72-771-2052
Submit Date2021-01-19
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-01-19
Release Version1
Akane Kanasaki Akane Kanasaki
https://dx.doi.org/10.21228/M8870S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001071
Project DOI:doi: 10.21228/M8870S
Project Title:D-Allulose effects on metabolomics profile in rodents
Project Summary:To determine the metabolomics profile after D-allulose intake.
Institute:Matsutani Chemical Industry Co., Ltd.
Last Name:Kanasaki
First Name:Akane
Address:5-3 Kita-Itami, Itami, Hyogo, 664-8508, Japan
Email:akane-kanasaki@matsutani.co.jp
Phone:81-72-771-2052

Subject:

Subject ID:SU001745
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Diet
SA152357C3control
SA152358C2control
SA152359C4control
SA152360C5control
SA152361C6control
SA152362C1control
SA152351P3D-Allulose
SA152352P4D-Allulose
SA152353P6D-Allulose
SA152354P2D-Allulose
SA152355P5D-Allulose
SA152356P1D-Allulose
Showing results 1 to 12 of 12

Collection:

Collection ID:CO001738
Collection Summary:The livers were collected and then flash-frozen in liquid N2
Sample Type:Liver

Treatment:

Treatment ID:TR001758
Treatment Summary:Seven-week old male Wistar rats were fed AIN-93G-based diets with/without 3% D-allulose, ad libitum for 4 weeks.

Sample Preparation:

Sampleprep ID:SP001751
Sampleprep Summary:For CE-TOFMS, The frozen tissue was plunged into 50% acetonitrile/Milli-Q water containing internal standards. The tissues were homogenized thrice, and then centrifuged. The upper aqueous layer was centrifugally filtered to remove the proteins. The filtrate was then centrifugally concentrated and re-suspended in 50 µL of Milli-Q water for CE-MS analysis. For LC-TOFMS, the frozen tissue was plunged into 1% formic acid/acetonitrile containing the internal standard solution. Tissue was homogenized thrice, and the mixture was yet again homogenized after adding Milli-Q water and then centrifuged. The supernatant was collected and 500 1% formic acid/acetonitrile and Milli-Q water were added to the precipitate. Homogenization and centrifugation were performed as described previously, and the supernatant was mixed with previously collected one. The mixed supernatant was filtrated to remove the proteins and phospholipids. The filtrate was then desiccated and dissolved in 200 µL of isopropanol/Milli-Q water for LC-MS analysis.

Combined analysis:

Analysis ID AN002720 AN002721 AN002722 AN002723
Analysis type MS MS MS MS
Chromatography type Other Other Reversed phase Reversed phase
Chromatography system Agilent CE Agilent CE Agilent 1200SL Agilent 1200SL
Column fused silica capillary (50 μm i.d. × 80 cm) fused silica capillary (50 μm i.d. × 80 cm) ODS column (2×50 mm, 2 μm) ODS column (2×50 mm, 2 μm)
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF QTOF QTOF
MS instrument name Agilent CE-TOFMS Agilent CE-TOFMS Agilent 6230 TOF Agilent 6230 TOF
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Relative Area Relative Area Relative Area Relative Area

Chromatography:

Chromatography ID:CH002008
Instrument Name:Agilent CE
Column Name:fused silica capillary (50 μm i.d. × 80 cm)
Chromatography Type:Other
  
Chromatography ID:CH002009
Instrument Name:Agilent 1200SL
Column Name:ODS column (2×50 mm, 2 μm)
Chromatography Type:Reversed phase

MS:

MS ID:MS002517
Analysis ID:AN002720
Instrument Name:Agilent CE-TOFMS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS002518
Analysis ID:AN002721
Instrument Name:Agilent CE-TOFMS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
  
MS ID:MS002519
Analysis ID:AN002722
Instrument Name:Agilent 6230 TOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS002520
Analysis ID:AN002723
Instrument Name:Agilent 6230 TOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
  logo