Summary of Study ST001915
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001208. The data can be accessed directly via it's Project DOI: 10.21228/M8JX2X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001915 |
| Study Title | Myocardial Rev-erb-mediated diurnal metabolic rhythm( Part1/3) |
| Study Summary | Agonists and antagonists of nuclear receptor Rev-erbα/β, key components of the circadian clock, can benefit the heart. Here, we show that mice with cardiomyocyte-specific knockout (KO) of Rev-erbα/β display progressive cardiac dilation and lethal heart failure. Inducible ablation of Rev-erbα/β in adult hearts causes similar phenotypes. Impaired fatty acid oxidation in the KO myocardium, particularly in the light cycle, precedes contractile dysfunctions with a reciprocal overreliance on carbohydrate utilization, particularly in the dark cycle. These findings delineate temporal coordination between clock-mediated anticipation and nutrient-induced response in myocardial metabolism. |
| Institute | Baylor College of Medicine |
| Last Name | Song |
| First Name | Shiyang |
| Address | One Baylor Plaza, Houston, Texas, 77030, USA |
| shiyangs@bcm.edu | |
| Phone | 7137983159 |
| Submit Date | 2021-09-10 |
| Raw Data Available | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-12-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001208 |
| Project DOI: | doi: 10.21228/M8JX2X |
| Project Title: | Myocardial Rev-erb-mediated diurnal metabolic rhythm |
| Project Summary: | Agonists and antagonists of nuclear receptor Rev-erbα/β, key components of the circadian clock, can benefit the heart. Here, we show that mice with cardiomyocyte-specific knockout (KO) of Rev-erbα/β display progressive cardiac dilation and lethal heart failure. Inducible ablation of Rev-erbα/β in adult hearts causes similar phenotypes. Impaired fatty acid oxidation in the KO myocardium, particularly in the light cycle, precedes contractile dysfunctions with a reciprocal overreliance on carbohydrate utilization, particularly in the dark cycle. These findings delineate temporal coordination between clock-mediated anticipation and nutrient-induced response in myocardial metabolism. |
| Institute: | Baylor College of Medicine |
| Last Name: | Song |
| First Name: | Shiyang |
| Address: | One Baylor Plaza, Houston, Texas, 77030, USA |
| Email: | shiyangs@bcm.edu |
| Phone: | 7137983159 |
Subject:
| Subject ID: | SU001993 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Group | Genotype | Experimental variables |
|---|---|---|---|---|
| SA177703 | KO_ZT10_3 | KO_ZT10 | Rev-erb cardiomyocyte specific knock out | KO mouse heart ventrcle harvest at ZT10 |
| SA177704 | KO_ZT10_1 | KO_ZT10 | Rev-erb cardiomyocyte specific knock out | KO mouse heart ventrcle harvest at ZT10 |
| SA177705 | KO_ZT10_2 | KO_ZT10 | Rev-erb cardiomyocyte specific knock out | KO mouse heart ventrcle harvest at ZT10 |
| SA177706 | KO_ZT22_2 | KO_ZT22 | Rev-erb cardiomyocyte specific knock out | KO mouse heart ventrcle harvest at ZT22 |
| SA177707 | KO_ZT22_1 | KO_ZT22 | Rev-erb cardiomyocyte specific knock out | KO mouse heart ventrcle harvest at ZT22 |
| SA177708 | KO_ZT22_3 | KO_ZT22 | Rev-erb cardiomyocyte specific knock out | KO mouse heart ventrcle harvest at ZT22 |
| SA177709 | WT_ZT10_1 | WT_ZT10 | wild type control | WT mouse heart ventrcle harvest at ZT10 |
| SA177710 | WT_ZT10_3 | WT_ZT10 | wild type control | WT mouse heart ventrcle harvest at ZT10 |
| SA177711 | WT_ZT10_2 | WT_ZT10 | wild type control | WT mouse heart ventrcle harvest at ZT10 |
| SA177712 | WT_ZT22_1 | WT_ZT22 | wild type control | WT mouse heart ventrcle harvest at ZT22 |
| SA177713 | WT_ZT22_2 | WT_ZT22 | wild type control | WT mouse heart ventrcle harvest at ZT22 |
| SA177714 | WT_ZT22_3 | WT_ZT22 | wild type control | WT mouse heart ventrcle harvest at ZT22 |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO001986 |
| Collection Summary: | Snap-frozen heart ventricles were collected from both WT and Rev-erb cardiomyocyte-specific KO mice at ZT10 or ZT22 |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR002005 |
| Treatment Summary: | no special treatment |
Sample Preparation:
| Sampleprep ID: | SP001999 |
| Sampleprep Summary: | Heart ventricle tissues were harvested from male mice at 3 months old (n = 3 at each condition) and were snap-frozen in liquid nitrogen. Lipids were extracted as previously described 55–57. Lipids were separated on a Shimadzu CTO-20A Nexera X2 UHPLC systems, 1.8 μm particle 50 × 2.1 mm Acquity HSS UPLC T3 column (Waters, Milford, MA). Lipidomics acquisition performed in both pos and neg mode ionization. Pos mode lipids were normalized by Internal Standard PC 17:0; [M+H]+, Neg mode lipids were normalized by Internal Standard PC 34:0. |
Chromatography:
| Chromatography ID: | CH002298 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Chromatography Type: | GC |
Analysis:
| Analysis ID: | AN003113 |
| Analysis Type: | MS |
| Chromatography ID: | CH002298 |
| Num Factors: | 4 |
| Num Metabolites: | 1941 |
| Units: | pmoles/l |
