Summary of Study ST001928

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001218. The data can be accessed directly via it's Project DOI: 10.21228/M88H76 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001928
Study TitleMetabolomics profiles of premenopausal women are different based on O-desmethylangolensin metabotype
Study SummaryUrinary O-desmethylangolensin (ODMA) concentrations provide a functional gut microbiome marker of dietary isoflavone daidzein metabolism to ODMA. Individuals who do not have gut microbial environments that produce ODMA have less favorable cardiometabolic and cancer risk profiles. Urinary metabolomics profiles were evaluated in relation to ODMA metabotypes within and between individuals over time. Secondary analysis was conducted of data from the BEAN2 trial, which was a cross-over study of premenopausal women consuming six months on a high- and a low-soy diet, each separated by a 1-month washout period. In all of the 672 samples in the study, 66 of the 84 women had the same ODMA metabotype at seven or all eight time points. Two or four urine samples per woman were selected based on temporal metabotypes in order to compare within and across individuals. Metabolomics assays for primary metabolism and biogenic amines were conducted in 60 urine samples from 20 women. Partial least-squares discriminant analysis was used to compare metabolomics profiles.
Institute
George Mason University
Last NameFrankenfeld
First NameCara
Address4400 University Drive, Fairfax, VA 22030
Emailprof.frankenfeld@gmail.com
Phone206-265-2563
Submit Date2021-08-20
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2021-10-29
Release Version1
Cara Frankenfeld Cara Frankenfeld
https://dx.doi.org/10.21228/M88H76
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001218
Project DOI:doi: 10.21228/M88H76
Project Title:Metabolomics profiles of premenopausal women are different based on O-desmethylangolensin metabotype
Project Summary:Urinary O-desmethylangolensin (ODMA) concentrations provide a functional gut microbiome marker of dietary isoflavone daidzein metabolism to ODMA. Individuals who do not have gut microbial environments that produce ODMA have less favorable cardiometabolic and cancer risk profiles. Urinary metabolomics profiles were evaluated in relation to ODMA metabotypes within and between individuals over time. Secondary analysis was conducted of data from the BEAN2 trial, which was a cross-over study of premenopausal women consuming six months on a high- and a low-soy diet, each separated by a 1-month washout period. In all of the 672 samples in the study, 66 of the 84 women had the same ODMA metabotype at seven or all eight time points. Two or four urine samples per woman were selected based on temporal metabotypes in order to compare within and across individuals. Metabolomics assays for primary metabolism and biogenic amines were conducted in 60 urine samples from 20 women. Partial least-squares discriminant analysis was used to compare metabolomics profiles.
Institute:George Mason University
Last Name:Frankenfeld
First Name:Cara
Address:4400 University Drive, Fairfax, VA 22030
Email:prof.frankenfeld@gmail.com
Phone:(206) 265-2563
Funding Source:Soy Health Research Program

Subject:

Subject ID:SU002006
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Organ Treatment Group ODMAmetabotye
SA178426Biorec004Plasma - - -
SA178427Biorec005Plasma - - -
SA178428Biorec003Plasma - - -
SA178429Biorec001Plasma - - -
SA178430Biorec002Plasma - - -
SA178431Biorec006Plasma - - -
SA178420MtdBlank002- - - -
SA178421MtdBlank001- - - -
SA178422MtdBlank004- - - -
SA178423MtdBlank003- - - -
SA178424MtdBlank005- - - -
SA178425MtdBlank006- - - -
SA178432B334u2.2Urine C0 C0 Nonproducer
SA178433B328u8.2Urine C0 C0 Nonproducer
SA178434B328u5.2Urine C0 C0 Nonproducer
SA178435B312u4.2Urine C0 C0 Nonproducer
SA178436B393u2.2Urine C0 C0 Nonproducer
SA178437B434u1.2Urine C0 C0 Nonproducer
SA178438B393u3.2Urine C0 C0 Nonproducer
SA178439B434u5.2Urine C0 C0 Nonproducer
SA178440B407u2.2Urine C0 C0 Nonproducer
SA178441B334u7.2Urine C0 C0 Nonproducer
SA178442B312u3.2Urine C0 C0 Nonproducer
SA178443B455u7.2Urine C0 C0 Nonproducer
SA178444B169u5.2Urine C0 C0 Nonproducer
SA178445B311u8.2Urine C0 C0 Nonproducer
SA178446B169u1.2Urine C0 C0 Nonproducer
SA178447B134u2.2Urine C0 C0 Nonproducer
SA178448B134u1.2Urine C0 C0 Nonproducer
SA178449B455u6.2Urine C0 C0 Nonproducer
SA178450B407u3.2Urine C0 C0 Nonproducer
SA178451B311u6.2Urine C0 C0 Nonproducer
SA178452B134u4.2Urine C0 C0 Producer
SA178453B134u5.2Urine C0 C0 Producer
SA178454B407u5.2Urine C1 C1 Producer
SA178455B455u5.2Urine C1 C1 Producer
SA178456B455u2.2Urine C1 C1 Producer
SA178457B407u6.2Urine C1 C1 Producer
SA178458B434u6.2Urine C1 C1 Producer
SA178459B434u7.2Urine C1 C1 Producer
SA178460B393u1.2Urine C1 C1 Producer
SA178461B312u5.2Urine C1 C1 Producer
SA178462B312u6.2Urine C1 C1 Producer
SA178463B311u2.2Urine C1 C1 Producer
SA178464B169u4.2Urine C1 C1 Producer
SA178465B393u8.2Urine C1 C1 Producer
SA178466B169u6.2Urine C1 C1 Producer
SA178467B328u2.2Urine C1 C1 Producer
SA178468B311u5.2Urine C1 C1 Producer
SA178469B328u3.2Urine C1 C1 Producer
SA178470B334u8.2Urine C1 C1 Producer
SA178471B334u5.2Urine C1 C1 Producer
SA178472B248u2.2Urine NC0 NC0 Nonproducer
SA178473B248u1.2Urine NC0 NC0 Nonproducer
SA178475B199u6.2Urine NC1 NC1 Producer
SA178476B199u7.2Urine NC1 NC1 Producer
SA178477B185u2.2Urine NC1 NC1 Producer
SA178478B234u1.2Urine NC1 NC1 Producer
SA178479B126u7.2Urine NC1 NC1 Producer
SA178480B126u2.2Urine NC1 NC1 Producer
SA178481B105u4.2Urine NC1 NC1 Producer
SA178482B185u5.2Urine NC1 NC1 Producer
SA178483B524u8.2Urine NC1 NC1 Producer
SA178484B431u5.2Urine NC1 NC1 Producer
SA178485B431u3.2Urine NC1 NC1 Producer
SA178486B105u3.1Urine NC1 NC1 Producer
SA178487B483u2.2Urine NC1 NC1 Producer
SA178488B483u7.2Urine NC1 NC1 Producer
SA178489B532u5.2Urine NC1 NC1 Producer
SA178490B532u1.2Urine NC1 NC1 Producer
SA178491B524u6.2Urine NC1 NC1 Producer
SA178492B234u6.2Urine NC1 NC1 Producer
SA178474B532u4.4Urine NC1 - -
Showing results 1 to 73 of 73

Collection:

Collection ID:CO001999
Collection Summary:Urine biospecimens were collected during cross-over trial among premenopausal women conducted from 2007-2010, and details about the study design and population are published (Morimoto et al 2014; Maskarinec et al 2011; Maskarinec et al 2012). The BEAN2 trial was a cross-over study with six months on a high- and a low-soy diet, each separated by a 1-month washout period.
Sample Type:Urine

Treatment:

Treatment ID:TR002018
Treatment Summary:As part of the parent study, daidzein, equol, and ODMA concentrations were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). These previously measured concentrations were used to classify metabotypes. Each urine sample was identified as being ODMA producer/non-producer and equol producer/non-producer based on a cut-off of equol/ODMA:daidzein ratio of 0.018. All samples, except two samples, which were excluded from selection, had daidzein concentrations >2 nmol/mg creatinine, indicating sufficient presence of the precursor metabolite for metabolite detection.

Sample Preparation:

Sampleprep ID:SP002012
Sampleprep Summary:Urine biospecimens were collected during cross-over trial among premenopausal women conducted from 2007-2010, and urine sample aliquots used for this analysis were kept frozen at -80C.

Combined analysis:

Analysis ID AN003136
Analysis type MS
Chromatography type HILIC
Chromatography system Waters Acquity
Column Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Unspecified
Ion Mode UNSPECIFIED
Units Peak height

Chromatography:

Chromatography ID:CH002318
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)
Chromatography Type:HILIC

MS:

MS ID:MS002916
Analysis ID:AN003136
Instrument Name:Unspecified
Instrument Type:QTOF
MS Type:ESI
MS Comments:See uploaded file.
Ion Mode:UNSPECIFIED
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