Summary of Study ST002176

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001384. The data can be accessed directly via it's Project DOI: 10.21228/M8TH8J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002176
Study TitleEffect of external high-dose rate radiation on mouse biofluid metabolomic signatures (neg)
Study SummaryIn the event of an improvised nuclear device (IND), a complex IR exposure will occur consisting of both low (LDR) and high-dose rates (HDR). We have previously addressed LDR exposures from internal emitters or externally deposited radionuclides on biofluid small molecule signatures, but further research on the HDR component is required. Here, we exposed 8 − 10 week old male C57BL/6 mice to a cumulative dose of 3 Gy using a reference dose rate of 0.7 Gy/min or a HDR of 7 Gy/sec, collected urine and serum at 1 and 7 d, then compared the metabolite signatures using an untargeted (urine) approach with liquid chromatography mass spectrometry platforms.
Institute
Georgetown University
Last NamePannkuk
First NameEvan
Address3970 Reservoir Rd, NW New Research Building E504
Emailelp44@georgetown.edu
Phone2026875650
Submit Date2022-04-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2023-06-01
Release Version1
Evan Pannkuk Evan Pannkuk
https://dx.doi.org/10.21228/M8TH8J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001384
Project DOI:doi: 10.21228/M8TH8J
Project Title:Effect of external high-dose rate radiation on mouse biofluid metabolomics
Project Summary:In the event of an improvised nuclear device (IND), a complex IR exposure will occur consisting of both low (LDR) and high-dose rates (HDR). We have previously addressed LDR exposures from internal emitters or externally deposited radionuclides on biofluid small molecule signatures, but further research on the HDR component is required. Here, we exposed 8 − 10 week old male C57BL/6 mice to a cumulative dose of 3 Gy using a reference dose rate of 0.7 Gy/min or a HDR of 7 Gy/sec, collected urine and serum at 1 and 7 d, then compared the metabolite signatures using a untargeted (urine) approache with liquid chromatography mass spectrometry platforms.
Institute:Georgetown University
Last Name:Pannkuk
First Name:Evan
Address:3970 Reservoir Rd, NW New Research Building E504
Email:elp44@georgetown.edu
Phone:2026875650
Publications:https://www.mdpi.com/2218-1989/12/6/520

Subject:

Subject ID:SU002262
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6
Gender:Male
Animal Animal Supplier:Charles River

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Factor Factor Factor
SA20903384CTL D1 post
SA209034113CTL D1 post
SA20903562CTL D1 post
SA20903616CTL D1 post
SA20903713CTL D1 post
SA20903814CTL D1 post
SA20903970CTL D1 pre
SA2090405CTL D1 pre
SA209041132CTL D1 pre
SA209042127CTL D1 pre
SA209043115CTL D1 pre
SA20904485CTL D1 pre
SA20904551CTL D7 post
SA20904678CTL D7 post
SA20904724CTL D7 post
SA209048120CTL D7 post
SA209049116CTL D7 pre
SA209050133CTL D7 pre
SA20905118CTL D7 pre
SA209052142CTL D7 pre
SA20905373CTL D7 pre
SA209054146CTL D7 pre
SA20905548HDR D1 post
SA209056108HDR D1 post
SA209057111HDR D1 post
SA209058139HDR D1 post
SA209059117HDR D1 post
SA209060114HDR D1 post
SA20906186HDR D1 pre
SA209062122HDR D1 pre
SA20906388HDR D1 pre
SA209064128HDR D1 pre
SA20906545HDR D1 pre
SA20906657HDR D1 pre
SA209067103HDR D7 post
SA209068145HDR D7 post
SA20906911HDR D7 post
SA20907054HDR D7 post
SA20907192HDR D7 post
SA209072129HDR D7 post
SA20907341HDR D7 pre
SA20907464HDR D7 pre
SA209075141HDR D7 pre
SA209076137HDR D7 pre
SA20907760HDR D7 pre
SA209078106REF D1 post
SA20907933REF D1 post
SA20908065REF D1 post
SA20908138REF D1 post
SA20908268REF D1 post
SA20908361REF D1 pre
SA209084151REF D1 pre
SA209085121REF D1 pre
SA20908634REF D1 pre
SA20908722REF D1 pre
SA20908830REF D7 post
SA20908926REF D7 post
SA20909096REF D7 post
SA209091150REF D7 post
SA209092131REF D7 post
SA20909340REF D7 post
SA20909477REF D7 pre
SA20909523REF D7 pre
SA209096125REF D7 pre
SA20909794REF D7 pre
SA20909817REF D7 pre
SA209099136REF D7 pre
Showing results 1 to 67 of 67

Collection:

Collection ID:CO002255
Collection Summary:Spot urine collected before and after irradiation
Sample Type:Urine
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002274
Treatment Summary:This novel irradiator is based on a Clinac 2100C (Varian Medical Systems, Corona, CA, USA) where the pulse delivery is controlled using in house software. All irradiations were performed using 9 MeV electrons with no scatterer or flattening filter. For these experiments, mice were placed in a 72 mm x 41mm x41mm acrylic box in which air holes had been drilled (The Container Store, Coppell, TX, USA). For 0.7 Gy/min irradiations, mice (n=6) were individually placed at 120 cm above the clinac head and irradiation delivered at 3.25 pulses per second. In this configuration, 3 Gy was delivered in 580 pulses. For 7 Gy/sec mice (n=6) were individually placed 20cm above the clinac head (Figure S1) and dose delivered at 180 pulses/sec after allowing 20 sec where the acceleration and electron source were both on but operated asynchronously so that no beam is delivered. In this configuration, 3 Gy was delivered in 78 pulses.

Sample Preparation:

Sampleprep ID:SP002268
Sampleprep Summary:[17,18]. Briefly, urine (20 μl) was deproteinized (80 μl 50% cold acetonitrile) with internal standards (2 μM debrisoquine [M+H]+ = 176.1188; 30 μM 4-nitrobenzoic acid [M-H]- = 166.0141), vortexed, incubated on ice (10 min), and centrifuged for 10 min (max speed, 4 °C). A 1 μl aliquot of each sample was combined for a quality control (QC) sample.
Processing Storage Conditions:-80℃

Combined analysis:

Analysis ID AN003564
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (50 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Synapt G2 S QTOF
Ion Mode NEGATIVE
Units peak area

Chromatography:

Chromatography ID:CH002635
Chromatography Summary:Mobile phases consisted of the following: solvent A (water/0.1% formic acid [FA]) and solvent B (acetonitrile/0.1% FA). The gradient for urine was (solvent A and B) 4.0 min 5% B, 4.0 min 20% B, 5.1 min 95% B, and 1.9 min 5% B at a flow rate of 0.5 ml/min, column temp 40 °C.
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (50 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:4.0 min 5% B, 4.0 min 20% B, 5.1 min 95% B, and 1.9 min 5% B
Flow Rate:0.5 ml/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS003321
Analysis ID:AN003564
Instrument Name:Waters Synapt G2 S QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Operating conditions for ESI were: capillary voltage 2.75 kV, cone voltage 30 V, desolvation temperature 500°C, desolvation gas flow 1000 L/Hr.
Ion Mode:NEGATIVE
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