Summary of Study ST002440

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001572. The data can be accessed directly via it's Project DOI: 10.21228/M8H70Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002440
Study TitleImpacts of interactions between environmental chemical exposures and diet on gut microbiota and microbiota-derived metabolites in mothers and children (MAAP Cohort)
Study TypeProspective Cohort Study
Study SummaryIn 2014, the Microbes, Allergy, Asthma and Pets (MAAP) study (P01 AI089473) was launched. All women ages 18-49 years scheduled for prenatal visits at Henry Ford Health, expected to deliver during the recruitment period at a Henry Ford Health hospital, and whose residence was in a predefined area of Detroit and metropolitan Detroit suburbs were potentially eligible. There were no medical insurance requirements. Between 2014 and 2016, 141 women were enrolled; their infant was followed through age 18 months (with continuing follow-up through early/mid childhood underway through the NIH ECHO program under the CREW birth cohort consortium (5UG3OD023282, Gern PI). Blood and stool samples were obtained from the mother prenatally and stool specimens were obtained from the infant at 5 time points (1-week, 1-, 3-, 6- and 18-months) with 16S sequencing already completed. Blood was also obtained from the infant at pre-defined intervals. Please contact Andrea Cassidy-Bushrow at acassid1@hfhs.org for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) OIF program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. MAAP is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
Wayne State University
DepartmentPharmacology
Last NamePetriello
First NameMichael
Address6135 Woodward Ave, Detroit, MI 48202
Emailmichael.petriello@wayne.edu
Phone313-577-1089
Submit Date2023-01-12
Total Subjects31
Study CommentsECHO Project EC0549, HHEAR Project E020-0012
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-01-12
Release Version1
Michael Petriello Michael Petriello
https://dx.doi.org/10.21228/M8H70Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001572
Project DOI:doi: 10.21228/M8H70Z
Project Title:Impacts of interactions between environmental chemical exposures and diet on gut microbiota and microbiota-derived metabolites in mothers and children
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) OIF program, and uses data from two cohorts: MAAP and MARCH. This project will examine associations with PFAS exposures and gut microbiota form and function in mothers and children over time. Untargeted metabolomics will be used as a means to assess changes in microbiota-derived metabolites.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Li
First Name:Yuanyuan
Address:Nutrition Research Institute, UNC-CH, 500 Laureate Way, Kannapolis, NC 28081
Email:yuanyli4@unc.edu
Phone:984-377-0693
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) OIF program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. MAAP is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; (U24 ES026539)

Subject:

Subject ID:SU002529
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA244064MAAP_HHEAR_1HHEAR Pool
SA244065MAAP_HHEAR_4HHEAR Pool
SA244066MAAP_HHEAR_3HHEAR Pool
SA244067MAAP_HHEAR_2HHEAR Pool
SA244068MAAP_NIST_4NIST Pool
SA244069MAAP_NIST_1NIST Pool
SA244070MAAP_NIST_3NIST Pool
SA244071MAAP_NIST_2NIST Pool
SA244072MAAP_SP_2Study Pool
SA244073MAAP_SP_1Study Pool
SA244074MAAP_SP_4Study Pool
SA244075MAAP_SP_3Study Pool
SA244076MAAP_S_23Study Sample
SA244077MAAP_S_24Study Sample
SA244078MAAP_S_22Study Sample
SA244079MAAP_S_20Study Sample
SA244080MAAP_S_21Study Sample
SA244081MAAP_S_25Study Sample
SA244082MAAP_S_26Study Sample
SA244083MAAP_S_31Study Sample
SA244084MAAP_S_19Study Sample
SA244085MAAP_S_30Study Sample
SA244086MAAP_S_29Study Sample
SA244087MAAP_S_28Study Sample
SA244088MAAP_S_27Study Sample
SA244089MAAP_S_14Study Sample
SA244090MAAP_S_5Study Sample
SA244091MAAP_S_6Study Sample
SA244092MAAP_S_7Study Sample
SA244093MAAP_S_4Study Sample
SA244094MAAP_S_3Study Sample
SA244095MAAP_S_1Study Sample
SA244096MAAP_S_2Study Sample
SA244097MAAP_S_8Study Sample
SA244098MAAP_S_9Study Sample
SA244099MAAP_S_15Study Sample
SA244100MAAP_S_16Study Sample
SA244101MAAP_S_17Study Sample
SA244102MAAP_S_13Study Sample
SA244103MAAP_S_12Study Sample
SA244104MAAP_S_10Study Sample
SA244105MAAP_S_11Study Sample
SA244106MAAP_S_18Study Sample
Showing results 1 to 43 of 43

Collection:

Collection ID:CO002522
Collection Summary:In 2014, the Microbes, Allergy, Asthma and Pets (MAAP) study (P01 AI089473) was launched. All women ages 18-49 years scheduled for prenatal visits at Henry Ford Health, expected to deliver during the recruitment period at a Henry Ford Health hospital, and whose residence was in a predefined area of Detroit and metropolitan Detroit suburbs were potentially eligible. There were no medical insurance requirements. Between 2014 and 2016, 141 women were enrolled; their infant was followed through age 18 months (with continuing follow-up through early/mid childhood underway through the NIH ECHO program under the CREW birth cohort consortium (5UG3OD023282, Gern PI). Blood and stool samples were obtained from the mother prenatally and stool specimens were obtained from the infant at 5 time points (1-week, 1-, 3-, 6- and 18-months) with 16S sequencing already completed. Blood was also obtained from the infant at pre-defined intervals. Please contact Andrea Cassidy-Bushrow at acassid1@hfhs.org for questions related to the subject characteristics and outcomes.
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002541
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002535
Sampleprep Summary:Study samples (500-1000 µL aliquots) were shipped from the Henry Ford Health System to the NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and aliquoted the study samples at 50 µL per aliquot. An additional 10 µL was taken from each study sample, mixed, and distributed at 50 µL per aliquot, which were used as QC study pools throughout the study. HHEAR reference plasma (50 µL each) and NIST plasma (1950) reference material (50 µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used as blank. All samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference plasma, NIST reference plasma, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extraction Method:Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
Extract Storage:-80℃
Sample Resuspension:Water-Methanol (95:5, v/v)
Sample Spiking:Tryptophan-d5 stock solution at 500 ng/mL

Combined analysis:

Analysis ID AN003977
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH002940
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000 psi
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 mL/min
Injection Temperature:8
Internal Standard:Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:10:90 Methanol:Water with 0.1% FA solution
Strong Wash Solvent Name:75:25 2-Propanol:Water with 0.1% FA solution
Chromatography Type:Reversed phase

MS:

MS ID:MS003711
Analysis ID:AN003977
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 kV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Temp:400°C
Fragmentation Method:CID
Desolvation Gas Flow:55
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