Summary of Study ST002455

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001583. The data can be accessed directly via it's Project DOI: 10.21228/M82X4B This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002455
Study TitleOrganism-Wide Analysis of Sepsis Reveals Mechanisms of Systemic Inflammation
Study Typelipidomics analysis
Study SummaryPhospholipase A2 group V (PLA2G5) is a secretory and Ca2+-dependent lipolytic enzyme and is inducible during several pathologic conditions. However, it has been unknown how PLA2G5 plays a role in sepsis. To study the role of PLA2G5 in sepsis, we performed lipidomics analysis of plasma and tissues from LPS-injected mice with or without PLA2G5 blockade. Here, we showed that PLA2G5 is involved in the production of fatty acids such as oleic acid and linoleic acid, lysophospholipids such as lysophosphatidic acid, lysophosphatidylcholine, lysophatidylethanolamine, and lysophosphatidylserine species, and metabolites derived from polyunsaturated fatty acids such as arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, and linoleic acids during sepsis. Thus, PLA2G5 regulates selective lipid pathways during sepsis.
Institute
University of Chicago
DepartmentPritzker School of Molecular Engineering
LaboratoryChevrier lab
Last NameTakahama
First NameMichihiro
Address900 E 57th St,, Chicago, Illinois, 60637, USA
Emailmtakahama@uchicago.edu
Phone7732302766
Submit Date2023-01-23
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-01-23
Release Version1
Michihiro Takahama Michihiro Takahama
https://dx.doi.org/10.21228/M82X4B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001583
Project DOI:doi: 10.21228/M82X4B
Project Title:Organism-Wide Analysis of Sepsis Reveals Mechanisms of Systemic Inflammation
Project Type:lipidomics analysis
Project Summary:Phospholipase A2 group V (PLA2G5) is a secretory and Ca2+-dependent lipolytic enzyme and is inducible during several pathologic conditions. However, it has been unknown how PLA2G5 plays a role in sepsis. To study the role of PLA2G5 in sepsis, we performed lipidomics analysis of plasma and tissues from LPS-injected mice with or without PLA2G5 blockade. Here, we showed that PLA2G5 is involved in the production of fatty acids such as oleic acid and linoleic acid, lysophospholipids such as lysophosphatidic acid, lysophosphatidylcholine, lysophatidylethanolamine, and lysophosphatidylserine species, and metabolites derived from polyunsaturated fatty acids such as arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, and linoleic acids during sepsis. Thus, PLA2G5 regulates selective lipid pathways during sepsis.
Institute:University of Chicago
Department:Pritzker School of Molecular Engineering
Laboratory:Chevrier lab
Last Name:Takahama
First Name:Michihiro
Address:900 E 57th St,, Chicago, Illinois, 60637, USA
Email:mtakahama@uchicago.edu
Phone:7732302766

Subject:

Subject ID:SU002544
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:6 weeks
Gender:Female
Animal Animal Supplier:JAX

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Treatment
SA245654BM_FFA_3Wild-type LPS
SA245655BM_FFA_4Wild-type LPS
SA245656BM_Oxylipin_2Wild-type LPS
SA245657BM_FFA_2Wild-type LPS
SA245658BM_Oxylipin_1Wild-type LPS
SA245659SP_FFA_1Wild-type LPS
SA245660CO_Oxylipin_2Wild-type LPS
SA245661CO_Oxylipin_3Wild-type LPS
SA245662CO_Oxylipin_4Wild-type LPS
SA245663BM_Oxylipin_3Wild-type LPS
SA245664BM_Oxylipin_4Wild-type LPS
SA245665PL_Oxylipin_2Wild-type LPS
SA245666PL_Oxylipin_3Wild-type LPS
SA245667PL_Oxylipin_4Wild-type LPS
SA245668PL_Oxylipin_1Wild-type LPS
SA245669PL_FFA_4Wild-type LPS
SA245670PL_FFA_1Wild-type LPS
SA245671PL_FFA_2Wild-type LPS
SA245672PL_FFA_3Wild-type LPS
SA245673CO_Oxylipin_1Wild-type LPS
SA245674BM_FFA_1Wild-type LPS
SA245675SP_Oxylipin_2Wild-type LPS
SA245676SP_Oxylipin_4Wild-type LPS
SA245677CO_FFA_1Wild-type LPS
SA245678SP_Oxylipin_1Wild-type LPS
SA245679SP_FFA_4Wild-type LPS
SA245680SP_FFA_2Wild-type LPS
SA245681SP_FFA_3Wild-type LPS
SA245682CO_FFA_2Wild-type LPS
SA245683SP_Oxylipin_3Wild-type LPS
SA245684CO_FFA_4Wild-type LPS
SA245685CO_FFA_3Wild-type LPS
SA245686PL_FFA_7Wild-type LPS+antiPla2g5
SA245687PL_FFA_8Wild-type LPS+antiPla2g5
SA245688PL_FFA_6Wild-type LPS+antiPla2g5
SA245689PL_FFA_5Wild-type LPS+antiPla2g5
SA245690SP_FFA_8Wild-type LPS+antiPla2g5
SA245691SP_FFA_7Wild-type LPS+antiPla2g5
SA245692SP_FFA_6Wild-type LPS+antiPla2g5
SA245693SP_FFA_5Wild-type LPS+antiPla2g5
SA245694PL_Oxylipin_8Wild-type LPS+antiPla2g5
SA245695CO_FFA_8Wild-type LPS+antiPla2g5
SA245696PL_Oxylipin_7Wild-type LPS+antiPla2g5
SA245697PL_Oxylipin_6Wild-type LPS+antiPla2g5
SA245698CO_FFA_6Wild-type LPS+antiPla2g5
SA245699PL_Oxylipin_5Wild-type LPS+antiPla2g5
SA245700CO_FFA_5Wild-type LPS+antiPla2g5
SA245701BM_Oxylipin_8Wild-type LPS+antiPla2g5
SA245702BM_FFA_5Wild-type LPS+antiPla2g5
SA245703BM_FFA_6Wild-type LPS+antiPla2g5
SA245704BM_FFA_7Wild-type LPS+antiPla2g5
SA245705SP_Oxylipin_7Wild-type LPS+antiPla2g5
SA245706SP_Oxylipin_8Wild-type LPS+antiPla2g5
SA245707CO_Oxylipin_7Wild-type LPS+antiPla2g5
SA245708CO_Oxylipin_6Wild-type LPS+antiPla2g5
SA245709BM_FFA_8Wild-type LPS+antiPla2g5
SA245710SP_Oxylipin_6Wild-type LPS+antiPla2g5
SA245711BM_Oxylipin_6Wild-type LPS+antiPla2g5
SA245712BM_Oxylipin_7Wild-type LPS+antiPla2g5
SA245713BM_Oxylipin_5Wild-type LPS+antiPla2g5
SA245714CO_FFA_7Wild-type LPS+antiPla2g5
SA245715SP_Oxylipin_5Wild-type LPS+antiPla2g5
SA245716CO_Oxylipin_5Wild-type LPS+antiPla2g5
SA245717CO_Oxylipin_8Wild-type LPS+antiPla2g5
Showing results 1 to 64 of 64

Collection:

Collection ID:CO002537
Collection Summary:C57BL/6J were injected intraperitoneally with 50 µg of anti-PLA2G5 neutralizing antibodies in 100 µl of PBS 1 hour prior to LPS injection. Twelve hours after LPS injection, mice were anesthetized with 2,2,2-tribromoethanol (250-500 mg/kg) and perfused transcardially with PBS containing 10 mM EDTA. Plasma was collected from blood prior to perfusion and frozen at -80°C. Immediately after perfusion, colon tissues were extensively washed in PBS and frozen by liquid nitrogen and kept at -80°C until the following procedures.
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002556
Treatment Summary:C57BL/6J were injected intraperitoneally with 50 µg of anti-PLA2G5 neutralizing antibodies in 100 µl of PBS 1 hour prior to LPS injection.

Sample Preparation:

Sampleprep ID:SP002550
Sampleprep Summary:Tissues were mechanically homogenized with the Precellys 24 homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France) in methanol containing internal standards (500 pmol/sample of d4-labeled EPA, d5-labeled PGE2, LPC with a 17:0 fatty acyl chain (LPC17:0), and PC with two 14:0 fatty acyl chains (PE14:0-14:0)) and then incubated overnight at -20°C. For extraction of phospholipids and lysophospholipids, one-tenth of tissue lysates were added to 10 volumes of 20 mM Tris-HCl (pH 7.4) and were extracted using the method of Bligh and Dyer. For extraction of oxygenated fatty acid metabolites, nine-tenths of the tissue lysates were added to water (final methanol concentration of 10% (v/v)), and the lipids were extracted using an Oasis HLB cartridge (Waters, Milford, MA, USA).

Combined analysis:

Analysis ID AN004005
Analysis type MS
Chromatography type Reversed phase
Chromatography system Shimadzu Nexera X2
Column Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 4000 QTrap
Ion Mode UNSPECIFIED
Units intensity

Chromatography:

Chromatography ID:CH002958
Instrument Name:Shimadzu Nexera X2
Column Name:Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)
Column Temperature:50
Flow Gradient:linear
Flow Rate:0.2 mL/min
Solvent A:acetonitrile/methanol/water = 1/1/1 (v/v/v) containing 5 mM phosphoric acid and 1 mM ammonium formate
Solvent B:2-propanol containing 5 uM phosphoric acid and 1 mM ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS003753
Analysis ID:AN004005
Instrument Name:ABI Sciex 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:The samples were applied to a Kinetex C18 column (Kinetex C18, 2.1 x 150 mm, 1.7 µm particle; Phenomenex, Inc., Torrance, CA, USA) connected with ESI-MS/MS on a liquid chromatography (NexeraX2 system; Shimadzu Co., Kyoto, Japan) coupled with a 4000Q-TRAP quadrupole-linear ion trap hybrid mass spectrometer (AB Sciex, Framingham, MA, USA). For analyses of free fatty acids (FFAs), lysophospholipids (LPLs) and phospholipids, the samples were applied to the column and separated by a step gradient with mobile phase A (acetonitrile/methanol/water =1/1/1 (v/v/v) containing 5 mM phosphoric acid and 1 mM ammonium formate) and mobile phase B (2-propanol containing 5 µM phosphoric acid and 1 mM ammonium formate) at a flow rate of 0.2 mL/min at 50°C. For analyses of oxygenated fatty acid metabolites, the samples were applied to the column and separated using a step gradient including mobile phase C (water containing 0.1 % acetic acid) and mobile phase D (acetonitrile/methanol = 4/1 (v/v)) at a flow rate of 0.2 mL/ min at 45°C. Identification of phospholipids, LPLs, FFAs, and oxygenated PUFAs (polyunsaturated fatty acids) metabolites was conducted by multiple reaction monitoring (MRM) transition, and quantification was performed based on the peak area of the MRM transition and the calibration curve obtained with an authentic standard for each compound.
Ion Mode:UNSPECIFIED
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