Summary of Study ST002662

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002662
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Liver - Targeted Acyl-CoA
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Duke University
DepartmentDuke Molecular Physiology Institute
LaboratoryMetabolomics Core Laboratory
Last NameNewgard
First NameChristopher
Address300 N Duke St, Durham, NC 27701
Emailchris.newgard@duke.edu
Phone(919) 668-6059
Submit Date2023-04-25
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Christopher Newgard Christopher Newgard
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002764
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26421890248016811Control 8 weeks of training or control time Female
SA26421990252016811Control 8 weeks of training or control time Female
SA26422090266016811Control 8 weeks of training or control time Female
SA26422190245016811Control 8 weeks of training or control time Female
SA26422290265016811Control 8 weeks of training or control time Female
SA26422390229016811Control 8 weeks of training or control time Male
SA26422490232016811Control 8 weeks of training or control time Male
SA26422590217016811Control 8 weeks of training or control time Male
SA26422690239016811Control 8 weeks of training or control time Male
SA26422790237016811Control 8 weeks of training or control time Male
SA26422880010816802_1QC-ExternalStandard 0 hour -
SA26422980011826802_2QC-ExternalStandard 0 hour -
SA26423080010816802_3QC-ExternalStandard 0 hour -
SA26423180011826802_1QC-ExternalStandard 0 hour -
SA26423280010816802_4QC-ExternalStandard 0 hour -
SA26423380011826802_3QC-ExternalStandard 0 hour -
SA26423480010816802_2QC-ExternalStandard 0 hour -
SA26423580011826802_4QC-ExternalStandard 0 hour -
SA26423690571016811Training 1 week of training or control time Female
SA26423790559016811Training 1 week of training or control time Female
SA26423890567016811Training 1 week of training or control time Female
SA26423990564016811Training 1 week of training or control time Female
SA26424090560016811Training 1 week of training or control time Female
SA26424190430016811Training 1 week of training or control time Male
SA26424290423016811Training 1 week of training or control time Male
SA26424390426016811Training 1 week of training or control time Male
SA26424490421016811Training 1 week of training or control time Male
SA26424590422016811Training 1 week of training or control time Male
SA26424690587016811Training 2 weeks of training Female
SA26424790578016811Training 2 weeks of training Female
SA26424890581016811Training 2 weeks of training Female
SA26424990576016811Training 2 weeks of training Female
SA26425090585016811Training 2 weeks of training Female
SA26425190441016811Training 2 weeks of training Male
SA26425290450016811Training 2 weeks of training Male
SA26425390439016811Training 2 weeks of training Male
SA26425490449016811Training 2 weeks of training Male
SA26425590444016811Training 2 weeks of training Male
SA26425690410016811Training 4 weeks of training Female
SA26425790420016811Training 4 weeks of training Female
SA26425890406016811Training 4 weeks of training Female
SA26425990416016811Training 4 weeks of training Female
SA26426090412016811Training 4 weeks of training Female
SA26426190292016811Training 4 weeks of training Male
SA26426290280016811Training 4 weeks of training Male
SA26426390283016811Training 4 weeks of training Male
SA26426490289016811Training 4 weeks of training Male
SA26426590281016811Training 4 weeks of training Male
SA26426690267016811Training 8 weeks of training or control time Female
SA26426790259016811Training 8 weeks of training or control time Female
SA26426890254016811Training 8 weeks of training or control time Female
SA26426990251016811Training 8 weeks of training or control time Female
SA26427090258016811Training 8 weeks of training or control time Female
SA26427190227016811Training 8 weeks of training or control time Male
SA26427290222016811Training 8 weeks of training or control time Male
SA26427390225016811Training 8 weeks of training or control time Male
SA26427490223016811Training 8 weeks of training or control time Male
SA26427590218016811Training 8 weeks of training or control time Male
Showing results 1 to 58 of 58

Collection:

Collection ID:CO002757
Collection Summary:-
Sample Type:Liver

Treatment:

Treatment ID:TR002773
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002770
Sampleprep Summary:500 ul of tissue homogenate preprared at 50 mg/ml in 50% acetonitrile/0.3% formic acid are spiked with C17 Acyl CoA. The acyl CoAs are purified by solid phase extraction using 2-(2-pyridyl) ethyl functionalized Silica gel (Sigma-Aldrich 54127-U).
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004334
Analysis type MS
Chromatography type Flow induction analysis
Chromatography system Waters Acquity UPLC
Column No column
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Waters Xevo TQ-S
Ion Mode POSITIVE
Units pmol/mg of tissue

Chromatography:

Chromatography ID:CH003240
Chromatography Summary:80% methanol/20% water containing 30 mM ammonium hydroxide is used as the mobile phase, the flow is 0.030 ml/min.
Methods Filename:pass1b_acoa_methods.pdf
Instrument Name:Waters Acquity UPLC
Column Name:No column
Chromatography Type:Flow induction analysis

MS:

MS ID:MS004081
Analysis ID:AN004334
Instrument Name:Waters Xevo TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Ion ratios of endogenous acyl CoAs to the C-17 CoA internal standard are computed from centroided spectra using a software package NeoLynx (Waters, Milford, MA). The ratios are converted to concentrations using calibrators prepared by spiking tissue homogenates with authentic CoAs (Sigma, St. Louis , MO) having saturated acyl chain lengths C0- C18. Corrections for the heavy isotope effects, mainly 13C, to the adjacent m+2 spectral peaks in a particular chain length cluster are made empirically by referring to the observed spectra for the analytical standards. The values are expressed in pmol/mg. Spectra are acquired in the multichannel acquisition mode monitoring the neutral loss of 507 amu (phosphoadenosine diphosphate) and scanning from m/z 750 to1060.
Ion Mode:POSITIVE
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