Summary of Study ST002671

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002671
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Lung - Untargeted Lipidomics, Reversed-Phase Positive
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Georgia Institute of Technology
DepartmentDepartment of Chemistry and Biochemistry
LaboratorySystems Mass Spectrometry Core (SyMSC)
Last NameFacundo
First NameFernandez
Address901 Atlantic Dr NW, Atlanta, GA 30332-0400
Emailfacundo.fernandez@chemistry.gatech.edu
Phone(404) 385-4432
Submit Date2023-05-02
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Fernandez Facundo Fernandez Facundo
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002773
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26475990265016607Control 8 weeks of training or control time Female
SA26476090245016607Control 8 weeks of training or control time Female
SA26476190248016607Control 8 weeks of training or control time Female
SA26476290266016607Control 8 weeks of training or control time Female
SA26476390252016607Control 8 weeks of training or control time Female
SA26476490239016607Control 8 weeks of training or control time Male
SA26476590229016607Control 8 weeks of training or control time Male
SA26476690232016607Control 8 weeks of training or control time Male
SA26476790217016607Control 8 weeks of training or control time Male
SA26476890237016607Control 8 weeks of training or control time Male
SA264769SampleBlank05QC-Blank 0 hour -
SA264770SampleBlank03QC-Blank 0 hour -
SA264771SampleBlank01QC-Blank 0 hour -
SA264772SampleBlank04QC-Blank 0 hour -
SA264773SampleBlank06QC-Blank 0 hour -
SA264774SampleBlank02QC-Blank 0 hour -
SA264775QC06QC-Pooled 0 hour -
SA264776QC08QC-Pooled 0 hour -
SA264777QC07QC-Pooled 0 hour -
SA264778QC05QC-Pooled 0 hour -
SA264779QC02QC-Pooled 0 hour -
SA264780QC09QC-Pooled 0 hour -
SA264781QC04QC-Pooled 0 hour -
SA264782QC01QC-Pooled 0 hour -
SA264783QC03QC-Pooled 0 hour -
SA264784SED_Reference_Lung02QC-Reference 0 hour -
SA264785SED_Reference_LungQC-Reference 0 hour -
SA264786IPE_Reference_LungQC-Reference 0 hour -
SA264787IPE_Reference_Lung02QC-Reference 0 hour -
SA26478890560016607Training 1 week of training or control time Female
SA26478990559016607Training 1 week of training or control time Female
SA26479090571016607Training 1 week of training or control time Female
SA26479190567016607Training 1 week of training or control time Female
SA26479290564016607Training 1 week of training or control time Female
SA26479390422016607Training 1 week of training or control time Male
SA26479490421016607Training 1 week of training or control time Male
SA26479590423016607Training 1 week of training or control time Male
SA26479690426016607Training 1 week of training or control time Male
SA26479790430016607Training 1 week of training or control time Male
SA26479890576016607Training 2 weeks of training Female
SA26479990581016607Training 2 weeks of training Female
SA26480090578016607Training 2 weeks of training Female
SA26480190587016607Training 2 weeks of training Female
SA26480290585016607Training 2 weeks of training Female
SA26480390450016607Training 2 weeks of training Male
SA26480490439016607Training 2 weeks of training Male
SA26480590444016607Training 2 weeks of training Male
SA26480690441016607Training 2 weeks of training Male
SA26480790449016607Training 2 weeks of training Male
SA26480890406016607Training 4 weeks of training Female
SA26480990416016607Training 4 weeks of training Female
SA26481090412016607Training 4 weeks of training Female
SA26481190420016607Training 4 weeks of training Female
SA26481290410016607Training 4 weeks of training Female
SA26481390289016607Training 4 weeks of training Male
SA26481490292016607Training 4 weeks of training Male
SA26481590280016607Training 4 weeks of training Male
SA26481690283016607Training 4 weeks of training Male
SA26481790281016607Training 4 weeks of training Male
SA26481890259016607Training 8 weeks of training or control time Female
SA26481990251016607Training 8 weeks of training or control time Female
SA26482090267016607Training 8 weeks of training or control time Female
SA26482190258016607Training 8 weeks of training or control time Female
SA26482290254016607Training 8 weeks of training or control time Female
SA26482390223016607Training 8 weeks of training or control time Male
SA26482490218016607Training 8 weeks of training or control time Male
SA26482590227016607Training 8 weeks of training or control time Male
SA26482690225016607Training 8 weeks of training or control time Male
SA26482790222016607Training 8 weeks of training or control time Male
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Collection:

Collection ID:CO002766
Collection Summary:-
Sample Type:Lung

Treatment:

Treatment ID:TR002782
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002779
Sampleprep Summary:IPA extraction with three cycles of freeze/thaw(with sonication)
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004343
Analysis type MS
Chromatography type Reversed phase
Chromatography system Vanquish horizon LC
Column Thermo Accucore C30 (100 x 2.1mm,2.6um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH003249
Chromatography Summary:global lipid separation
Methods Filename:pass1b_lrpneg_lrppos_methods.pdf
Instrument Name:Vanquish horizon LC
Column Name:Thermo Accucore C30 (100 x 2.1mm,2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=1 min: 40%/60% (%A/%B) @ 0.4 ml/min | t=5 min: 30%/70% (%A/%B) @ 0.4 ml/min | t=8 min: 10%/90% (%A/%B) @ 0.4 ml/min | t=8.2 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.5 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.7 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=12 min: 80%/20% (%A/%B) @ 0.4 ml/min
Flow Rate:0.4 ml/min
Solvent A:40% water/60% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Solvent B:10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004090
Analysis ID:AN004343
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
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