Summary of Study ST002678

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002678
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Liver - Untargeted Lipidomics, Reversed-Phase Positive
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Georgia Institute of Technology
DepartmentDepartment of Chemistry and Biochemistry
LaboratorySystems Mass Spectrometry Core (SyMSC)
Last NameFacundo
First NameFernandez
Address901 Atlantic Dr NW, Atlanta, GA 30332-0400
Emailfacundo.fernandez@chemistry.gatech.edu
Phone(404) 385-4432
Submit Date2023-05-02
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Fernandez Facundo Fernandez Facundo
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002780
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26530190266016809Control 8 weeks of training or control time Female
SA26530290252016809Control 8 weeks of training or control time Female
SA26530390265016809Control 8 weeks of training or control time Female
SA26530490245016809Control 8 weeks of training or control time Female
SA26530590248016809Control 8 weeks of training or control time Female
SA26530690237016809Control 8 weeks of training or control time Male
SA26530790217016809Control 8 weeks of training or control time Male
SA26530890232016809Control 8 weeks of training or control time Male
SA26530990229016809Control 8 weeks of training or control time Male
SA26531090239016809Control 8 weeks of training or control time Male
SA265311SampleBlank05QC-Blank 0 hour -
SA265312SampleBlank04QC-Blank 0 hour -
SA265313SampleBlank03QC-Blank 0 hour -
SA265314SampleBlank06QC-Blank 0 hour -
SA265315SampleBlank02QC-Blank 0 hour -
SA265316SampleBlank01QC-Blank 0 hour -
SA265317QC08QC-Pooled 0 hour -
SA265318QC04QC-Pooled 0 hour -
SA265319QC07QC-Pooled 0 hour -
SA265320QC03QC-Pooled 0 hour -
SA265321QC09QC-Pooled 0 hour -
SA265322QC02QC-Pooled 0 hour -
SA265323QC05QC-Pooled 0 hour -
SA265324QC01QC-Pooled 0 hour -
SA265325QC06QC-Pooled 0 hour -
SA265326SED_Reference_Liver02QC-Reference 0 hour -
SA265327IPE_Reference_LiverQC-Reference 0 hour -
SA265328IPE_Reference_Liver02QC-Reference 0 hour -
SA265329SED_Reference_LiverQC-Reference 0 hour -
SA26533090567016809Training 1 week of training or control time Female
SA26533190564016809Training 1 week of training or control time Female
SA26533290560016809Training 1 week of training or control time Female
SA26533390571016809Training 1 week of training or control time Female
SA26533490559016809Training 1 week of training or control time Female
SA26533590422016809Training 1 week of training or control time Male
SA26533690430016809Training 1 week of training or control time Male
SA26533790423016809Training 1 week of training or control time Male
SA26533890426016809Training 1 week of training or control time Male
SA26533990421016809Training 1 week of training or control time Male
SA26534090578016809Training 2 weeks of training Female
SA26534190585016809Training 2 weeks of training Female
SA26534290581016809Training 2 weeks of training Female
SA26534390576016809Training 2 weeks of training Female
SA26534490587016809Training 2 weeks of training Female
SA26534590441016809Training 2 weeks of training Male
SA26534690439016809Training 2 weeks of training Male
SA26534790450016809Training 2 weeks of training Male
SA26534890444016809Training 2 weeks of training Male
SA26534990449016809Training 2 weeks of training Male
SA26535090410016809Training 4 weeks of training Female
SA26535190412016809Training 4 weeks of training Female
SA26535290416016809Training 4 weeks of training Female
SA26535390406016809Training 4 weeks of training Female
SA26535490420016809Training 4 weeks of training Female
SA26535590292016809Training 4 weeks of training Male
SA26535690283016809Training 4 weeks of training Male
SA26535790280016809Training 4 weeks of training Male
SA26535890281016809Training 4 weeks of training Male
SA26535990289016809Training 4 weeks of training Male
SA26536090251016809Training 8 weeks of training or control time Female
SA26536190259016809Training 8 weeks of training or control time Female
SA26536290267016809Training 8 weeks of training or control time Female
SA26536390254016809Training 8 weeks of training or control time Female
SA26536490258016809Training 8 weeks of training or control time Female
SA26536590227016809Training 8 weeks of training or control time Male
SA26536690225016809Training 8 weeks of training or control time Male
SA26536790218016809Training 8 weeks of training or control time Male
SA26536890223016809Training 8 weeks of training or control time Male
SA26536990222016809Training 8 weeks of training or control time Male
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Collection:

Collection ID:CO002773
Collection Summary:-
Sample Type:Liver

Treatment:

Treatment ID:TR002789
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002786
Sampleprep Summary:IPA extraction with three cycles of freeze/thaw(with sonication)
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004350
Analysis type MS
Chromatography type Reversed phase
Chromatography system Vanquish horizon LC
Column Thermo Accucore C30 (100 x 2.1mm,2.6um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH003256
Chromatography Summary:global lipid separation
Methods Filename:pass1b_lrpneg_lrppos_methods.pdf
Instrument Name:Vanquish horizon LC
Column Name:Thermo Accucore C30 (100 x 2.1mm,2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=1 min: 40%/60% (%A/%B) @ 0.4 ml/min | t=5 min: 30%/70% (%A/%B) @ 0.4 ml/min | t=8 min: 10%/90% (%A/%B) @ 0.4 ml/min | t=8.2 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.5 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.7 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=12 min: 80%/20% (%A/%B) @ 0.4 ml/min
Flow Rate:0.4 ml/min
Solvent A:40% water/60% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Solvent B:10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004097
Analysis ID:AN004350
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
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