Summary of Study ST002719

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001686. The data can be accessed directly via it's Project DOI: 10.21228/M8S13H This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002719
Study TitleComparison of metabolic of A549 cells before and after Gossypol acetate (GAA) treatment
Study SummaryGAA is a natural product with anti-cancer application prospect, but its anti-tumor molecular mechanism is still controversial. Previous studies have showed that GAA can suppress the expression of mitochondrial DNA encoded proteins by targeting LRPPRC, suggesting that GAA may affect mitochondrial metabolism. Here, metabonomics was applied to study of effect of GAA on central carbon metabolism in A549 cells. The metabolomic data showed that GAA significantly decreased tricarboxylic acid cycle ralated metabolites and significantly increased glycolysis-related metabolites. These results indicated that GAA could inhibite oxidative phosphorylation in A549 cells.
Institute
Hangzhou Institute of Medicine (HIM), University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Chinese Academy of Sciences
Last NameZhou
First NameWei
AddressBanshan Road
Emailzhouwei1989@iccas.ac.cn
Phone057188122431
Submit Date2023-05-23
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-06-21
Release Version1
Wei Zhou Wei Zhou
https://dx.doi.org/10.21228/M8S13H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001686
Project DOI:doi: 10.21228/M8S13H
Project Title:Comparison of metabolic of A549 cells before and after Gossypol acetate (GAA) treatment
Project Summary:A549 cells were seeded into 6-well cell culture dishes and treated with DMSO or 10 μM GAA for 48 h. Analysis of energy metabolites using liquid chromatography-mass spectrometry.
Institute:Hangzhou Institute of Medicine (HIM), University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Chinese Academy of Sciences
Last Name:Zhou
First Name:Wei
Address:Banshan Road
Email:zhouwei1989@iccas.ac.cn
Phone:057188122431

Subject:

Subject ID:SU002824
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA273731A549-GAA-310μM
SA273732A549-GAA-210μM
SA273733A549-GAA-110μM
SA273734A549-NC-2Control
SA273735A549-NC-3Control
SA273736A549-NC-1Control
Showing results 1 to 6 of 6

Collection:

Collection ID:CO002817
Collection Summary:Cultured cells were collected by trypsin digestion and low-speed centrifugation. The collected cells were rapidly cooled using liquid nitrogen, and the cooled samples were stored in a cryogenic refrigerator at -80 degrees Celsius.
Sample Type:Lung

Treatment:

Treatment ID:TR002833
Treatment Summary:A549 cells were seeded in six-well plates and cultured at 37 °C and 5% CO2. When the cell confluence reached about 60%, the corresponding final concentration of GAA or equivalent volume of DMSO was added. The final volume of the medium was 3 ml/well, and the treatment time was 48 hours.

Sample Preparation:

Sampleprep ID:SP002830
Sampleprep Summary:The collected cells were thawed on ice, then added 500 μM pre-cooled extractant (80% methanol aqueous solution), and whirl for 2 min. Freeze the mixture for 5 min in liquid nitrogen after remove ice for 5 min, it will be whirled for 2 min, circulate this at 3 times. Centrifuge the mixture again with 15000 rpm/min at 4 ℃ for 20 min. Finally take the supernatant into the sample bottle for LC-MS/MS analysis.

Combined analysis:

Analysis ID AN004408
Analysis type MS
Chromatography type HILIC
Chromatography system Nexera UHPLC LC-30A
Column Merck SeQuant ZIC-pHILIC (100 x 2.1mm,5um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex API 6500 QTrap
Ion Mode POSITIVE
Units relative intensity

Chromatography:

Chromatography ID:CH003308
Instrument Name:Nexera UHPLC LC-30A
Column Name:Merck SeQuant ZIC-pHILIC (100 x 2.1mm,5um)
Column Temperature:40℃
Flow Gradient:0min A/B (5:95 V/V), 9.5min (50:50 V/V), 11.1 min（5:95 V/V）,14 min (5:95 V/V))
Flow Rate:0.4 ml/min
Solvent A:10mmol/L ammonium acetate+0.3% ammonia
Solvent B:90% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS004156
Analysis ID:AN004408
Instrument Name:ABI Sciex API 6500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:The data collection system mainly includes ultra-high performance liquid chromatography and tandem mass spectrometry. LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer(QTRAP), QTRAP® LC-MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operatingin positive and negative ion mode and controlled by Analyst 1.6.3 software (Sciex). The ESI source operation parameters were as follows: source temperature 450 ∘C; ion spray voltage (IS) 5500 V (positive), -4500 V (negative); ion source gas I (GSI), gas II (GSII), curtain gas (CUR) were set at 40, 55, and 35.0 psi, respectively; the collision gas (CAD) was medium. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
Ion Mode:POSITIVE
Analysis Protocol File:Protocol.pdf
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