Summary of Study ST002749

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001712. The data can be accessed directly via it's Project DOI: 10.21228/M8DM8V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002749
Study TitleBromeliad lipidomics for adaptation to elevation
Study SummaryUntargeted analysis on Bromeliades leaf samples. The aim of this study was to evaluate the lipid profile alterations on Pitcairnia flammea leaves based on the different altitudes where they were collected. A lipidomic approach was applied to the samples. Ultra-high performance liquid chromatography coupled to electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw data and MS-DIAL was used to perform data preprocessing. The statistical analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization of a trend distribution based on the altitude. Our study grouped the individuals into three major groups: one with individuals from the lower elevations (UBA, RAN, and COR), another with individuals from the highest elevations (PAP and MAR), and another with individuals from mixed localities from intermediate elevations. Higher elevation population showed an increase in very long chain fatty acids and monounsaturated lipids compared to lower elevation as a response to cold higher elevations affecting the leaves' membrane fluidity. In addition, the higher elevation population showed a higher abundance of hexosylceramides and phosphatidylglycerol compared to lower ones.
Institute
University of Campinas
DepartmentChemistry's Institute
LaboratoryLaboratory of Bioanalytics and Integrated Omics
Last NameMatos
First NameTaynara
AddressRua Josué de Castro, s/n – Cidade Universitária, 13083-970, Campinas – SP, Brazil
Emailt262827@dac.unicamp.br
Phone(85)996154192
Submit Date2023-06-20
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-08-07
Release Version1
Taynara Matos Taynara Matos
https://dx.doi.org/10.21228/M8DM8V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001712
Project DOI:doi: 10.21228/M8DM8V
Project Title:Bromeliad lipidomics for adaptation to elevation
Project Type:MS untargeted analysis
Project Summary:Untargeted analysis on Bromeliades leaf samples. The aim of this study was to evaluate the lipid profile alterations on Pitcairnia flammea leaves based on the different altitudes where they were collected. A lipidomic approach was applied to the samples. Ultra-high performance liquid chromatography coupled to electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw data and MS-DIAL was used to perform data preprocessing. The statistical analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization of a trend distribution based on the altitude.
Institute:University of Campinas
Department:Chemistry's Institute
Laboratory:Laboratory of Bioanalytics and Integrated Omics
Last Name:Matos
First Name:Taynara
Address:Rua Josué de Castro, s/n – Cidade Universitária, 13083-970, Campinas – SP, Brazil
Email:t262827@dac.unicamp.br
Phone:(85)996154192

Subject:

Subject ID:SU002856
Subject Type:Plant
Subject Species:Pitcairnia flammea

Factors:

Subject type: Plant; Subject species: Pitcairnia flammea (Factor headings shown in green)

mb_sample_id local_sample_id Collection locale
SA289311COR192-POSCOR
SA289312COR191-POSCOR
SA289313COR195-POSCOR
SA289314COR197-POSCOR
SA289315COR199-POSCOR
SA289316COR186-NEGCOR
SA289317COR186-POSCOR
SA289318COR197-NEGCOR
SA289319COR192-NEGCOR
SA289320COR191-NEGCOR
SA289321COR199-NEGCOR
SA289322COR195-NEGCOR
SA289323ITA116-NEGITA
SA289324ITA113-NEGITA
SA289325ITA119-NEGITA
SA289326ITA102-NEGITA
SA289327ITA110-POSITA
SA289328ITA113-POSITA
SA289329ITA115-NEGITA
SA289330ITA119-POSITA
SA289331ITA116-POSITA
SA289332ITA115-POSITA
SA289333ITA110-NEGITA
SA289334ITA102-POSITA
SA289335MAC158-NEGMAC
SA289336MAC163-NEGMAC
SA289337MAC153-NEGMAC
SA289338MAC153-POSMAC
SA289339MAC155-POSMAC
SA289340MAC155-NEGMAC
SA289341MAC163-POSMAC
SA289342MAC158-POSMAC
SA289343MAC152-NEGMAC
SA289344MAC152-POSMAC
SA289345MAR131-NEGMAR
SA289346MAR134-NEGMAR
SA289347MAR139-NEGMAR
SA289348MAR128-NEGMAR
SA289349MAR131-POSMAR
SA289350MAR155-POSMAR
SA289351MAR139-POSMAR
SA289352MAR134-POSMAR
SA289353MAR155-NEGMAR
SA289354MAR128-POSMAR
SA289355PAP17-NEGPAP
SA289356PAP01-NEGPAP
SA289357PAP04-NEGPAP
SA289358PAP04-POSPAP
SA289359PAP15-POSPAP
SA289360PAP24-NEGPAP
SA289361PAP24-POSPAP
SA289362PAP17-POSPAP
SA289363PAP15-NEGPAP
SA289364PAP01-POSPAP
SA289365PES145-NEGPES
SA289366PES148-NEGPES
SA289367PES149-NEGPES
SA289368PES142-NEGPES
SA289369PES145-POSPES
SA289370PES150-POSPES
SA289371PES149-POSPES
SA289372PES148-POSPES
SA289373PES150-NEGPES
SA289374PES142-POSPES
SA289375QC04-POSPooled QC
SA289376QC03-POSPooled QC
SA289377QC02-POSPooled QC
SA289378QC01-NEGPooled QC
SA289379QC05-POSPooled QC
SA289380QC06-POSPooled QC
SA289381QC010-POSPooled QC
SA289382QC09-POSPooled QC
SA289383QC08-POSPooled QC
SA289384QC07-POSPooled QC
SA289385QC03-NEGPooled QC
SA289386QC01-POSPooled QC
SA289387QC06-NEGPooled QC
SA289388QC010-NEGPooled QC
SA289389QC05-NEGPooled QC
SA289390QC07-NEGPooled QC
SA289391QC08-NEGPooled QC
SA289392QC02-NEGPooled QC
SA289393QC09-NEGPooled QC
SA289394QC04-NEGPooled QC
SA289395RAN167-POSRAN
SA289396RAN169-POSRAN
SA289397RAN178-POSRAN
SA289398RAN175-NEGRAN
SA289399RAN177-POSRAN
SA289400RAN175-POSRAN
SA289401RAN174-POSRAN
SA289402RAN173-POSRAN
SA289403RAN167-NEGRAN
SA289404RAN177-NEGRAN
SA289405RAN174-NEGRAN
SA289406RAN173-NEGRAN
SA289407RAN178-NEGRAN
SA289408RAN169-NEGRAN
SA289409UBA05-POSUBA
SA289410UBA04-POSUBA
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Collection:

Collection ID:CO002849
Collection Summary:Pitcairnia flammea individuals were collected in different altitudes locations and named according to the local. PAP was collected at 2140 m, MAR 2037 m, PES 1496 m, ITA 1139 m, MAC 959 m, COR 425 m, RAN 25 m and UBA 20 m. Then, the plants were cultivated at the Institute of Biology (UNICAMP, Brazil). The mid-region of the youngest fully expanded leaves of five to seven individuals per population of healthy plants with visually appealing leaves were harvested, and immediately frozen in liquid nitrogen, followed the -80ºC storage until sample preparation.
Sample Type:Leaves
Collection Location:Institute of Biology (UNICAMP, Brazil)

Treatment:

Treatment ID:TR002865
Treatment Summary:Leaves were macerated to fine powder using a mortar and pestle in liquid nitrogen condition.

Sample Preparation:

Sampleprep ID:SP002862
Sampleprep Summary:Lipid extraction was based on Hummel et al. Method. 60 mL of solvent mixture was prepared with pre-cooled (-20ºC) methanol (MeOH, grade HPLC, LiChrosolv® Reag. Ph. Eur.) and methyl-tert-butyl-ether (MTBE, grade HPLC, purity 99,9%, Sigma-Aldrich) in proportion (1:3 v/v). In a 2 mL tube, 50 mg of macerated sample were added and 1 mL of the solvent mixture. The samples were incubated for 5 min under agitation at 500 rpm at 4 ºC (Microtube Shaking Incubator AccuTherm, Labnet International, Inc.), followed by an ultrasonication (Branson 5800 Ultrasonic Bath, Emerson, Danbury, USA) in ice-cold bath in 10 minutes. After adding 500 μL mixture of water type I:MeOH (3:1 v/v), the samples were vortexed and centrifuged for 5 min at 4 ºC, 10000 rpm (Hettich Zentrifugen Mikro 220R, Tuttlingen, DE). The three phases were separated and dried in a vacuum concentrator (Concentrator Plus, Eppendorf AG, Hamburg, DE), at ambient temperature under vacuum - alcoholic mode, and stored at -80 ºC until the chromatographic analysis.
Sampleprep Protocol Filename:SamplePrep-BromeliadLipidomics.pdf

Combined analysis:

Analysis ID AN004459 AN004460
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific UltiMate™ 3000 UHPLC RSLCnano system Thermo Scientific UltiMate™ 3000 UHPLC RSLCnano system
Column Supelco Sigma-Aldrich Titan C18 (100 x 2.1mm, 1.9um) Supelco Sigma-Aldrich Titan C18 (100 x 2.1mm, 1.9um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE POSITIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH003348
Methods Filename:UntargetedRP-BromeliadLipidomics.pdf
Instrument Name:Thermo Scientific UltiMate™ 3000 UHPLC RSLCnano system
Column Name:Supelco Sigma-Aldrich Titan C18 (100 x 2.1mm, 1.9um)
Column Temperature:40
Flow Gradient:250 µL/min: 0-2 min: 40% B, 2-3 min: 50% B, 3-6 min: 50% B, 6.1-8 min: 70% B, 8-9 min: 100% B, 9-11 min: 100% B, 11-12 min: 40% B, 12-14 min: 40% B.
Flow Rate:250 µL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/90% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004206
Analysis ID:AN004459
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
Analysis Protocol File:UntargetedRP-BromeliadLipidomics.pdf
  
MS ID:MS004207
Analysis ID:AN004460
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
Analysis Protocol File:UntargetedRP-BromeliadLipidomics.pdf
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