Summary of Study ST002794

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001742. The data can be accessed directly via it's Project DOI: 10.21228/M8J71N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002794
Study Title	Metabolomics Study on Plasma and Lung Tissue of Rats Exposed to Whole Thorax Irradiation.
Study Summary	Although some progress has been made in the study of radiation injury, there are still no effective prevention and treatment methods for severe acute radiation syndrome or sickness (ARS). Accordingly, a thorough understanding of biological characteristics associated with high-dose radiation is essential for revealing the mechanisms underlying the varied biological processes following high dose radiation and the development of novel potent radioprotective agents. In present study, plasma metabolic characteristics were investigated from patients of hematopoietic stem cell transplantation following high-dose TBI pretreatment utilizing gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). The most potential panel of four metabolic markers for radiation injury was selected and the metabolic disorders involved were explored. The metabolic disorders implied the dysregulation of gut microflora, shift of energy supply from aerobic respiration to ketogenesis, protein synthesis and metabolism in response to TBI. Although similar metabolic alternation patterns exist between male and female following high-dose irradiation, specific changes are observed in either male or female patients. These findings provide valuable information for further selecting biomarkers, clues of pathogenic mechanisms involved in high-dose radiation exposure.
Institute	Soochow University
Last Name	Wang
First Name	Chang
Address	Suzhou, No. 199, Renai Road, Suzhou Industrial Park
Email	wangchang@suda.edu.cn
Phone	+8651265880067
Submit Date	2023-07-18
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2023-08-10
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR001742
Project DOI:	doi: 10.21228/M8J71N
Project Title:	Metabolomics Study on Plasma and Lung Tissue of Rats Exposed to Whole Thorax Irradiation
Project Type:	MS quantitative analysis
Project Summary:	Although some progress has been made in the study of radiation injury, there are still no effective prevention and treatment methods for severe acute radiation syndrome or sickness (ARS). Accordingly, a thorough understanding of biological characteristics associated with high-dose radiation is essential for revealing the mechanisms underlying the varied biological processes following high dose radiation and the development of novel potent radioprotective agents. Therefore, in this paper, we investigated the metabolic characteristics of plasma and lung tissue of rats exposed to whole-thorax irradiation (WTI) by liquid chromatography-mass spectrometry (LC-MS). The correlation of metabolic characteristics between plasma and lung tissue of WTI rats was studied. Parallel metabolic pathway analysis of common differential metabolites suggests that the metabolic characteristics of plasma can reflect the metabolic changes of RILI. On this basis, the potential plasma biomarkers of RILI (acylcarnitine C9:1, acylcarnitine C5:0, taurocholic acid and tauro-α-Muricholic acid) were screened, and their dose-response patterns suggest that they have the potential for early diagnosis and evaluation of RILI.
Institute:	Soochow University
Last Name:	Wang
First Name:	Chang
Address:	Suzhou, No. 199, Renai Road, Suzhou Industrial Park
Email:	wangchang@suda.edu.cn
Phone:	+86-512-65880067

Subject:

Subject ID:	SU002901
Subject Type:	Mammal
Subject Species:	Rattus norvegicus
Gender:	Female

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment
SA299574	20210203-C4-6-5h	Control
SA299575	20210203-C3-6-5h	Control
SA299576	20210203-C5-6-5h	Control
SA299577	20210322-C1-3-72h	Control
SA299578	20210203-C6-1-5h	Control
SA299579	20210203-C8-2-5h	Control
SA299580	20210203-C6-6-5h	Control
SA299581	20210203-C6-4-5h	Control
SA299582	20210322-C1-4-72h	Control
SA299583	20210322-C1-6-72h	Control
SA299584	20210322-C6-1-72h	Control
SA299585	20210322-C6-4-72h	Control
SA299586	20210322-C6-6-72h	Control
SA299587	20210322-C8-2-72h	Control
SA299588	20210322-C5-6-72h	Control
SA299589	20210322-C4-6-72h	Control
SA299590	20210322-C2-1-72h	Control
SA299591	20210322-C3-3-72h	Control
SA299592	20210322-C3-6-72h	Control
SA299593	20210203-C8-6-5h	Control
SA299594	20210308-C3-3-72h	Control
SA299595	20210326-C2-1-48h	Control
SA299596	20210326-C3-3-48h	Control
SA299597	20210326-C3-6-48h	Control
SA299598	20210326-C1-6-48h	Control
SA299599	20210326-C1-4-48h	Control
SA299600	20210331-C1-3-24h	Control
SA299601	20210318-C1-3-5h	Control
SA299602	20210326-C1-3-48h	Control
SA299603	20210326-C4-6-48h	Control
SA299604	20210326-C5-6-48h	Control
SA299605	20210308-C1-4-72h	Control
SA299606	20210308-C1-6-72h	Control
SA299607	20210308-C2-1-72h	Control
SA299608	20210308-C1-3-72h	Control
SA299609	20210326-C8-6-48h	Control
SA299610	20210326-C6-1-48h	Control
SA299611	20210326-C6-4-48h	Control
SA299612	20210326-C8-2-48h	Control
SA299613	20210322-C8-6-72h	Control
SA299614	20210203-C3-3-5h	Control
SA299615	20210324-C1-3-120h	Control
SA299616	20210324-C1-4-120h	Control
SA299617	20210324-C1-6-120h	Control
SA299618	20210308-C8-6-72h	Control
SA299619	20210311-C1-3-120h	Control
SA299620	20210311-C2-1-120h	Control
SA299621	20210311-C1-6-120h	Control
SA299622	20210311-C1-4-120h	Control
SA299623	20210324-C2-1-120h	Control
SA299624	20210324-C3-3-120h	Control
SA299625	20210324-C6-6-120h	Control
SA299626	20210324-C8-2-120h	Control
SA299627	20210324-C8-6-120h	Control
SA299628	20210324-C6-4-120h	Control
SA299629	20210324-C6-1-120h	Control
SA299630	20210324-C3-6-120h	Control
SA299631	20210324-C4-6-120h	Control
SA299632	20210324-C5-6-120h	Control
SA299633	20210311-C3-3-120h	Control
SA299634	20210311-C3-6-120h	Control
SA299635	20210308-C4-6-72h	Control
SA299636	20210308-C5-6-72h	Control
SA299637	20210308-C6-1-72h	Control
SA299638	20210308-C3-6-72h	Control
SA299639	20210203-C1-3-5h	Control
SA299640	20210203-C2-1-5h	Control
SA299641	20210203-C1-6-5h	Control
SA299642	20210203-C1-4-5h	Control
SA299643	20210308-C6-4-72h	Control
SA299644	20210308-C6-6-72h	Control
SA299645	20210311-C6-1-120h	Control
SA299646	20210311-C5-6-120h	Control
SA299647	20210311-C4-6-120h	Control
SA299648	20210311-C6-4-120h	Control
SA299649	20210311-C6-6-120h	Control
SA299650	20210308-C8-2-72h	Control
SA299651	20210311-C8-6-120h	Control
SA299652	20210311-C8-2-120h	Control
SA299653	20210331-C1-6-24h	Control
SA299654	20210326-C6-6-48h	Control
SA299655	20210206-C5-6-48h	Control
SA299656	20210206-C6-1-48h	Control
SA299657	20210206-C6-4-48h	Control
SA299658	20210206-C6-6-48h	Control
SA299659	20210206-C4-6-48h	Control
SA299660	20210206-C3-6-48h	Control
SA299661	20210206-C1-4-48h	Control
SA299662	20210206-C1-6-48h	Control
SA299663	20210206-C2-1-48h	Control
SA299664	20210206-C3-3-48h	Control
SA299665	20210206-C8-2-48h	Control
SA299666	20210206-C8-6-48h	Control
SA299667	20210329-C5-6-24h	Control
SA299668	20210329-C6-1-24h	Control
SA299669	20210329-C6-4-24h	Control
SA299670	20210329-C6-6-24h	Control
SA299671	20210329-C4-6-24h	Control
SA299672	20210329-C3-6-24h	Control
SA299673	20210329-C1-3-24h	Control

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Collection:

Collection ID:	CO002894
Collection Summary:	Whole blood was collected about 5 hours, 1 day, 2 days, 3 days, and 5 days before and after irradiation, and tissue samples were taken on the fifth day。
Sample Type:	Blood (whole)

Treatment:

Treatment ID:	TR002910
Treatment Summary:	After anesthesia with 10% chloral hydrate solution (0.25 ml/100 g) by intraperitoneal injection, the small animal radiation therapy simulator (X-RAD SmART) was used to irradiate the whole lung tissue using a 40×40 mm lead collimator. Whole chest X-ray irradiation of 10 Gy, 20 Gy and 35 Gy in each dose group was given by anterior-anterior-field cross-irradiation, and the dose rate was about 2.7 Gy/min. The control group was also anesthetized, but not irradiated

Treatment ID:

TR002910

Treatment Summary:

After anesthesia with 10% chloral hydrate solution (0.25 ml/100 g) by intraperitoneal injection, the small animal radiation therapy simulator (X-RAD SmART) was used to irradiate the whole lung tissue using a 40×40 mm lead collimator. Whole chest X-ray irradiation of 10 Gy, 20 Gy and 35 Gy in each dose group was given by anterior-anterior-field cross-irradiation, and the dose rate was about 2.7 Gy/min. The control group was also anesthetized, but not irradiated

Sample Preparation:

Sampleprep ID:	SP002907
Sampleprep Summary:	Plasma：Add 200 μL ice acetonitrile containing internal standard (internal standard and its concentration are shown in Table 1-3) to 50 μL plasma, swirl and mix well for 2 min, centrifuge at 13,000 rpm and 4 ℃ for 15 min, and divide the supernatant into two parts in parallel (100 μL each for determination and analysis of positive and negative ion modes of LC-MS). Freeze-dried and stored in a -80 ℃ refrigerator. Before metabolomics analysis, the samples were redissolved with 50 μL 25% acetonitrile/water (v/v) solution。 Tissue:Weigh about 20 mg lung tissue sample powder into 2 mL EP tube, add 1.5 mL 80% methanol/water (v/v) solution containing internal standard (internal standard and its concentration are shown in Table 2-1), swirl and mix well for 2 min, then add 3 zirconium beads into the sample and grind at 45 hz for 2 times, 5 min each time. The supernatant was divided into two parts in parallel (600 μL each for determination of positive and negative ion modes of LC-MS) at an interval of 15 s, and then centrifuged at 13,000 rpm for 15 min at 4 ℃. The supernatant was freeze-dried and stored in a refrigerator at -80 ℃. Before metabolomics analysis, samples were redissolved with 100 μL 80% methanol/water (v/v) solution.

Sampleprep ID:

SP002907

Sampleprep Summary:

Plasma：Add 200 μL ice acetonitrile containing internal standard (internal standard and its concentration are shown in Table 1-3) to 50 μL plasma, swirl and mix well for 2 min, centrifuge at 13,000 rpm and 4 ℃ for 15 min, and divide the supernatant into two parts in parallel (100 μL each for determination and analysis of positive and negative ion modes of LC-MS). Freeze-dried and stored in a -80 ℃ refrigerator. Before metabolomics analysis, the samples were redissolved with 50 μL 25% acetonitrile/water (v/v) solution。 Tissue:Weigh about 20 mg lung tissue sample powder into 2 mL EP tube, add 1.5 mL 80% methanol/water (v/v) solution containing internal standard (internal standard and its concentration are shown in Table 2-1), swirl and mix well for 2 min, then add 3 zirconium beads into the sample and grind at 45 hz for 2 times, 5 min each time. The supernatant was divided into two parts in parallel (600 μL each for determination of positive and negative ion modes of LC-MS) at an interval of 15 s, and then centrifuged at 13,000 rpm for 15 min at 4 ℃. The supernatant was freeze-dried and stored in a refrigerator at -80 ℃. Before metabolomics analysis, samples were redissolved with 100 μL 80% methanol/water (v/v) solution.

Combined analysis:

Analysis ID	AN004545	AN004546
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	Thermo TSQ Vantage HPLC-MS/MS	Thermo TSQ Vantage HPLC-MS/MS
Column	Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)	Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um)
MS Type	ESI	ESI
MS instrument type	Triple quadrupole	Triple quadrupole
MS instrument name	Thermo TSQ Vantage	Thermo TSQ Vantage
Ion Mode	NEGATIVE	POSITIVE
Units	peak area	peak area

Chromatography:

Chromatography ID:	CH003414
Chromatography Summary:	negtive ion mode
Instrument Name:	Thermo TSQ Vantage HPLC-MS/MS
Column Name:	Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:	55
Flow Gradient:	0~1 min, 2% B; 1-20 min, 2~100% B; 20~24min, 100% B; 24~24.5min, 100~2% B; 24.5~34 min, 2% B
Flow Rate:	0.35 mL/min
Solvent A:	100% water; 0.1% formic acid
Solvent B:	100% acetonitrile; 0.1% formic acid
Chromatography Type:	Reversed phase

Chromatography ID:	CH003415
Chromatography Summary:	positive ion mode
Instrument Name:	Thermo TSQ Vantage HPLC-MS/MS
Column Name:	Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um)
Column Temperature:	50
Flow Gradient:	0~0.5min, 5% B; 0.5 ~24min, 5~100% B; 24-28 min, 40-100% B; 28~28.5min, 100~5% B; 28.5~48 min, 5% B
Flow Rate:	0.35 mL/min
Solvent A:	water(0.1% formic acid )
Solvent B:	acetonitrile(0.1% formic acid )
Chromatography Type:	Reversed phase

MS:

MS ID:	MS004292
Analysis ID:	AN004545
Instrument Name:	Thermo TSQ Vantage
Instrument Type:	Triple quadrupole
MS Type:	ESI
MS Comments:	The temperature of ion source and ion transport tube is 350 ℃, the electrospray voltage in positive and negative ion mode is -2,500 V respectively, the sheath pressure is 35 Arb, and the auxiliary pressure is 10 Arb. Data acquisition and pre-processing and instrument interface control are performed in Xcalibur (Thermo, USA) software.
Ion Mode:	NEGATIVE

MS ID:	MS004293
Analysis ID:	AN004546
Instrument Name:	Thermo TSQ Vantage
Instrument Type:	Triple quadrupole
MS Type:	ESI
MS Comments:	The temperature of ion source and ion transport tube is 350 ℃, the electrospray voltage in positive and negative ion mode is 3,000 V respectively, the sheath pressure is 35 Arb, and the auxiliary pressure is 10 Arb. Data acquisition and pre-processing and instrument interface control are performed in Xcalibur (Thermo, USA) software.
Ion Mode:	POSITIVE