Summary of Study ST002815

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001748. The data can be accessed directly via it's Project DOI: 10.21228/M8RQ7M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002815
Study TitleInvestigation of metabolism in hypertrophic cardiomyopathy - HILIC
Study SummaryWe propose the use of targeted metabolomics to define the metabolic and molecular pathways altered in 2 mouse models (R92W-TnT and R403Q-MHC) of hypertrophic cardiomyopathy (HCM) that span the spectrum of human disease (heart failure and sudden death), with the goal of identifying treatment targets. Parallel targeted metabolomics studies will be performed in heart tissue and plasma at rest and following inotrope stimulation. Since energy compromise is expected to be most marked when the heart is subject to increased workload, as is the case during high-intensity exercise or inotropic stimulation, we propose metabolomics studies in heart tissue and plasma, at rest and following inotrope stimulation. We anticipate that the results of these studies will allow us to move forward with further investigations into specific metabolites of interest as biomarkers, to be tested in HCM patients in future studies.
Institute
University of California, San Francisco
DepartmentHypertrophic Cardiomyopathy Center of Excellence
LaboratoryUCSF Smith Cardiovascular Research Building
Last NameAbraham
First NameMaria Roselle
Address555 Mission Bay Boulevard South, San Francisco, CA 94158
EmailRoselle.Abraham@ucsf.edu
Phone415-502-3911
Submit Date2023-07-27
Num Groups2 groups of 2 (i.e., mutant vs control of two mouse models of HCM (R402Q-MyHC, R92W-TnT)
Total Subjects32
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2023-09-06
Release Version1
Maria Roselle Abraham Maria Roselle Abraham
https://dx.doi.org/10.21228/M8RQ7M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001748
Project DOI:doi: 10.21228/M8RQ7M
Project Title:Investigation of metabolism in hypertrophic cardiomyopathy
Project Summary:We propose the use of targeted metabolomics to define the metabolic and molecular pathways altered in 2 mouse models (R92W-TnT and R403Q-MHC) of hypertrophic cardiomyopathy (HCM) that span the spectrum of human disease (heart failure and sudden death), with the goal of identifying treatment targets. Parallel targeted metabolomics studies will be performed in heart tissue and plasma at rest and following inotrope stimulation. Since energy compromise is expected to be most marked when the heart is subject to increased workload, as is the case during high-intensity exercise or inotropic stimulation, we propose metabolomics studies in heart tissue and plasma, at rest and following inotrope stimulation. We anticipate that the results of these studies will allow us to move forward with further investigations into specific metabolites of interest as biomarkers, to be tested in HCM patients in future studies.
Institute:University of California, San Francisco
Department:Hypertrophic Cardiomyopathy Center of Excellence
Laboratory:UCSF Smith Cardiovascular Research Building
Last Name:Abraham
First Name:Maria Roselle
Address:555 Mission Bay Boulevard South, San Francisco, CA 94158
Email:Roselle.Abraham@ucsf.edu
Phone:415-502-3911
Contributors:Arpana Vaniya (avaniya@ucdavis.edu), Anja Karlstaedt (Anja.Karlstaedt@cshs.org)

Subject:

Subject ID:SU002924
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA302519M503Control MHC
SA302520M438Control MHC
SA302521M499Control MHC
SA302522M492Control MHC
SA302523M440Control MHC
SA302524M485Control MHC
SA302525M447Control MHC
SA302526M487Control MHC
SA302527T545Control TNT
SA302528T547Control TNT
SA302529T544Control TNT
SA302530T518Control TNT
SA302531T515Control TNT
SA302532T524Control TNT
SA302533T520Control TNT
SA302534T522Control TNT
SA302543MtdBlank002Method Blank
SA302544MtdBlank001Method Blank
SA302545MtdBlank003Method Blank
SA302546MtdBlank005Method Blank
SA302547MtdBlank007Method Blank
SA302548MtdBlank004Method Blank
SA302549MtdBlank006Method Blank
SA302535M486MHC
SA302536M483MHC
SA302537M540MHC
SA302538M498MHC
SA302539M504MHC
SA302540M439MHC
SA302541M500MHC
SA302542M441MHC
SA302550Biorec006QC
SA302551Biorec005QC
SA302552Biorec007QC
SA302553Biorec002QC
SA302554Biorec001QC
SA302555Biorec003QC
SA302556Biorec004QC
SA302557T523TNT
SA302558T519TNT
SA302559T525TNT
SA302560T521TNT
SA302561T540TNT
SA302562T546TNT
SA302563T542TNT
SA302564T527TNT
Showing results 1 to 46 of 46

Collection:

Collection ID:CO002917
Collection Summary:Excision while the heart still beating and flash freeze within 15 seconds of excision
Sample Type:Heart

Treatment:

Treatment ID:TR002933
Treatment Summary:Saline IP and heparine SC injection in control & mutant group. After 10 minutes of saline injection we performed cervical dislocation, whole heart excision and flash freeze within 15 seconds of excision.

Sample Preparation:

Sampleprep ID:SP002930
Sampleprep Summary:2 mg of lyophilized tissue extracted with 1 mL 3:3:2 ACN/IPA/H2O 450 uL of extract dried and resuspended in 80:20 ACN/H2O.

Combined analysis:

Analysis ID AN004582
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1290
Column Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple TOF
MS instrument name ABI Sciex 6600 TripleTOF
Ion Mode POSITIVE
Units peak heights

Chromatography:

Chromatography ID:CH003443
Chromatography Summary:Column: Waters Acquity UPLC BEH Amide Column, 1.7 μm, 2.1 mm x 150 mm); Pre-column: Waters Acquity UPLC BEH Amide VanGuard pre-column (1.7 μm , 5 mm × 2.1 mm;)
Instrument Name:Agilent 1290
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:40C
Flow Gradient:0 min 100% (B), 0-2 min 100% (B), 2-7 min 70% (B), 7.7-9 min 40% (B), 9.5-10.25 min 30% (B), 10.25- 12.75 min 100% (B), 16.75 min 100% (B)
Flow Rate:0.4 mL/min
Solvent A:Ultrapure water with 10 mM ammonium formiate + 0.125% formic acid, pH 3
Solvent B:95:5 v/v acetonitrile:ultrapure water w/ 10 mM ammonium formiate + 0.125% formic acid, pH 3
Chromatography Type:HILIC

MS:

MS ID:MS004328
Analysis ID:AN004582
Instrument Name:ABI Sciex 6600 TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:The triple time-of-flight (TTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 50-1500 in positive mode. Instrument parameters are as follows: Gas Temp 500°C, Ion Source Gas 1 50, Ion Source Gas 2 50, Curtain Gas 34, Ion Spray Voltage 4000 V. Data was collected in centroid mode, cycle time 0.5 seconds for a total of 1679 cycles. Full MS-ddMS/MS was acquired.
Ion Mode:POSITIVE
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