Summary of Study ST002870

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001793. The data can be accessed directly via it's Project DOI: 10.21228/M8Z433 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002870
Study TitleA carbon-nitrogen negative feedback loop underlies the repeated evolution of cnidarian-Symbiodiniaceae symbioses across >700 Myr
Study SummarySymbiotic associations with Symbiodiniaceae have evolved independently across a diverse range of cnidarian taxa including reef-building corals, sea anemones, and jellyfish, yet the molecular mechanisms underlying their regulation and repeated evolution are still elusive. Here we show that despite their independent evolution, cnidarian hosts employ the same carbon-nitrogen negative feedback loop to control symbiont proliferation. Symbiont-derived photosynthates are used to assimilate nitrogenous waste via GS/GOGAT mediated amino acid biosynthesis in a carbon dependent manner, which regulates the availability of nitrogen to the symbionts. Using nutrient supplementation experiments, we show that the provision of additional carbohydrates significantly reduces symbiont density while ammonium promotes symbiont proliferation. UHPLC-HR-MS analysis confirmed that all hosts co-incorporated glucose-derived 13C and ammonium-derived 15N via GS/GOGAT mediated amino acid biosynthesis. Our results reveal a general carbon-nitrogen negative feedback loop underlying these symbioses and provide a parsimonious explanation for their repeated evolution.
Institute
King Abdullah University of Science and Technology
Last NameCui
First NameGuoxin
Address4700 KAUST, 23955-6900 Thuwal
Emailguoxin.cui@kaust.edu.sa
Phone+966544701749
Submit Date2023-09-12
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-10-04
Release Version1
Guoxin Cui Guoxin Cui
https://dx.doi.org/10.21228/M8Z433
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001793
Project DOI:doi: 10.21228/M8Z433
Project Title:A carbon-nitrogen negative feedback loop underlies the repeated evolution of cnidarian-Symbiodiniaceae symbioses across >700 Myr
Project Summary:Using nutrient supplementation experiments, we show that the provision of additional carbohydrates significantly reduces symbiont density while ammonium promotes symbiont proliferation. UHPLC-HR-MS analysis confirmed that all hosts co-incorporated glucose-derived 13C and ammonium-derived 15N via GS/GOGAT mediated amino acid biosynthesis. Our results reveal a general carbon-nitrogen negative feedback loop underlying these symbioses and provide a parsimonious explanation for their repeated evolution.
Institute:King Abdullah University of Science and Technology
Last Name:Cui
First Name:Guoxin
Address:4700 KAUST, 23955-6900 Thuwal
Email:guoxin.cui@kaust.edu.sa
Phone:+966544701749

Subject:

Subject ID:SU002982
Subject Type:Invertebrate
Subject Species:Multiple Cnidarian species: Exaiptasia diaphana, Stylophora pistillata, Cassiopea andromeda

Factors:

Subject type: Invertebrate; Subject species: Multiple Cnidarian species: Exaiptasia diaphana, Stylophora pistillata, Cassiopea andromeda (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA313088SymAip_13C15N413CG+15NA
SA313089SymAip_13C15N313CG+15NA
SA313090SymAip_13C15N213CG+15NA
SA313091SymCas_13C15N113CG+15NA
SA313092ApoAip_13C15N113CG+15NA
SA313093SymCas_13C15N413CG+15NA
SA313094SymCas_13C15N313CG+15NA
SA313095SymAip_13C15N113CG+15NA
SA313096SymCas_13C15N213CG+15NA
SA313097ApoAip_13C15N313CG+15NA
SA313098ApoAip_13C15N213CG+15NA
SA313099ApoAip_13C15N413CG+15NA
SA313100SymCas_13C113CGG
SA313101ApoAip_13C213CGG
SA313102SymAip_13C513CGG
SA313103SymCas_13C213CGG
SA313104ApoAip_13C113CGG
SA313105SymSty_13C113CGG
SA313106SymSty_13C513CGG
SA313107SymSty_13C613CGG
SA313108SymSty_13C413CGG
SA313109SymSty_13C313CGG
SA313110SymSty_13C213CGG
SA313111SymAip_13C413CGG
SA313112SymCas_13C313CGG
SA313113ApoAip_13C513CGG
SA313114SymAip_13C113CGG
SA313115ApoAip_13C413CGG
SA313116ApoAip_13C313CGG
SA313117SymAip_13C213CGG
SA313118SymAip_13C313CGG
SA313119SymSty_13C15N213CGG+15NA
SA313120SymSty_13C15N613CGG+15NA
SA313121SymSty_13C15N513CGG+15NA
SA313122SymSty_13C15N413CGG+15NA
SA313123SymSty_13C15N313CGG+15NA
SA313124SymSty_13C15N113CGG+15NA
SA313125SymSty_15N415NA
SA313126SymSty_15N115NA
SA313127SymSty_15N315NA
SA313128SymSty_15N515NA
SA313129SymAip_15N415NA
SA313130SymAip_15N115NA
SA313131SymAip_15N215NA
SA313132SymAip_15N315NA
SA313133SymSty_15N215NA
SA313134SymCas_15N415NA
SA313135ApoAip_15N115NA
SA313136ApoAip_15N415NA
SA313137ApoAip_15N315NA
SA313138ApoAip_15N215NA
SA313139SymCas_15N115NA
SA313140SymCas_15N315NA
SA313141SymCas_15N215NA
SA313142SymSty_15N615NA
SA313143SymSty_Con3Con
SA313144SymSty_Con6Con
SA313145SymSty_Con5Con
SA313146SymSty_Con4Con
SA313147SymSty_Con2Con
SA313148SymSty_Con1Con
SA313149ApoAip_Con5Con
SA313150SymCas_Con2Con
SA313151SymCas_Con3Con
SA313152SymCas_Con1Con
SA313153SymAip_Con3Con
SA313154SymAip_Con4Con
SA313155SymAip_Con2Con
SA313156SymAip_Con1Con
SA313157ApoAip_Con2Con
SA313158ApoAip_Con3Con
SA313159ApoAip_Con4Con
SA313160SymAip_Con5Con
SA313161ApoAip_Con1Con
Showing results 1 to 74 of 74

Collection:

Collection ID:CO002975
Collection Summary:Coral fragments and jellyfish were collected from the Red Sea and maintained in the lab for more than 6 months before the experiment. Sea anemone Exaiptasia were maintained in the lab for more than 5 years before the experiment.
Sample Type:Whole invertebrate animal

Treatment:

Treatment ID:TR002991
Treatment Summary:S. pistillata fragments, symbiotic and aposymbiotic E. diaphana polyps, and C. andromeda at the medusa stage were incubated for 48 hours with either filtered seawater, filtered seawater with 10 mM 13C6-glucose, filtered seawater with 250 µM 15N-ammonium, or filtered seawater and 10 mM 13C6-glucose and 250 µM 15N-ammonium.

Sample Preparation:

Sampleprep ID:SP002988
Sampleprep Summary:Animal tissue homogenates were lysed in 5 ml milli-Q water and lyophilized using a freeze dryer (Labconco). The lyophilisates were resuspended in 1 ml pre-chilled (-20 ℃) 100% methanol, sonicated for 30 minutes at 4 ℃ in an ultrasonication bath (Branson), and centrifuged at 3,000g for 30 minutes at 4 ℃. The supernatants were collected and stored in -80 ℃. The pellets were resuspended in 1 ml 50% methanol (-20 ℃) and centrifuged at 3,000g for 30 minutes at 4 ℃. The supernatants were then combined with those collected from the previous step. The total extracts were then centrifuged at 16,000g for 15 minutes at 4 ℃ to remove any potential particulates. The supernatants were dried using a speed vacuum concentrator (Labconco) and stored at -80 ℃ until further processing.

Combined analysis:

Analysis ID AN004704
Analysis type MS
Chromatography type HILIC
Chromatography system Dionex Ultimate 3000 UHPLC
Column Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH003542
Instrument Name:Dionex Ultimate 3000 UHPLC
Column Name:Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Column Temperature:35
Flow Gradient:0–8 min, 95 % B to 25 % B; 8–11 min, 25 % B; 11–12 min, 25 % B to 95 % B; 12–15 min, 95 % B
Flow Rate:0.25 mL/min
Solvent A:100% water; 0.01% formic acid
Solvent B:100% acetonitrile; 0.01% formic acid
Chromatography Type:HILIC

MS:

MS ID:MS004450
Analysis ID:AN004704
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Amino acids were detected using a mass spectrometer operated in positive mode with a spray voltage of 3.0 kV, sheath gas flow rate of 35 arbitrary units, auxiliary gas flow rate of 10 arbitrary units, spray capillary temperature of 300 °C, auxiliary gas heater temperature of 325 °C, AGC target of 3e6, and resolution of 280,000. Xcalibur software was used for the MS data acquisition, peak identification, signal extraction, and the related quantifications.
Ion Mode:POSITIVE
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