Summary of Study ST003098
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003098 |
| Study Title | Lactiplantibacillus plantarum intervention on cecum tryptophans of gestational fecal microbiome transplant germ-free mice |
| Study Summary | The study aims to investigate whether administration of probiotic L. plantarum could influence profiling of cecum tryptophans of germ-free mice. L. plantarum intervention during gestation of humanized GF mice showed no significant difference on tryptophans’s content. |
| Institute | Beijing Sanyuan Foods Co. Ltd |
| Last Name | Lijun |
| First Name | Chen |
| Address | 8th yingchang street, yinghai district |
| chenlijun@sanyuan.com.cn | |
| Phone | 01056306594 |
| Submit Date | 2024-02-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001923 |
| Project DOI: | doi: 10.21228/M85149 |
| Project Title: | Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice |
| Project Summary: | The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism. |
| Institute: | Beijing Sanyuan Foods Co. Ltd |
| Last Name: | Lijun |
| First Name: | Chen |
| Address: | 8th yingchang street, yinghai distric |
| Email: | chenlijun@sanyuan.com.cn |
| Phone: | 01056306594 |
Subject:
| Subject ID: | SU003213 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA333341 | 908MN | control |
| SA333342 | 185MN | control |
| SA333343 | 188MN | control |
| SA333344 | 909MN | control |
| SA333345 | 122MN | intervention |
| SA333346 | 120MN | intervention |
| SA333347 | 907MN | intervention |
| SA333348 | 180MN | intervention |
| SA333349 | 81MN | intervention |
| SA333350 | 107MN | intervention |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003206 |
| Collection Summary: | Cecum content was collected and stored at -80°C environment for tryptophan analysis. |
| Sample Type: | Cecum |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003222 |
| Treatment Summary: | Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003219 |
| Sampleprep Summary: | Sample was extracted in 100 μL of 80% methanol aqueous solution, high throughput tissue grinder grinding for 60 s, then add 900 μL of 10% methanol aqueous solution, high throughput tissue grinder grinding for 120 s. Centrifugation was carried out at 12,000 rpm at 4 ℃ for 10 min, and the supernatant was filtered through the membrane to add an equal amount of the internal standard solution, and then transferred into the vial. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Chromatography:
| Chromatography ID: | CH003828 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-2 min, 1% B; 2-3 min, 1-30% B; 3-3.5 min, 30% B; 4.5-8 min, 30-50% B; 8-10 min, 50-95% B; 10-11 min, 95% B; 11-17 min, 95-1% B. |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in methanol |
| Chromatography Type: | LC |
Analysis:
| Analysis ID: | AN005069 |
| Analysis Type: | MS |
| Chromatography ID: | CH003828 |
| Num Factors: | 2 |
| Num Metabolites: | 30 |
| Units: | μg/g |
