Summary of Study ST003804

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002378. The data can be accessed directly via it's Project DOI: 10.21228/M87Z5M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003804
Study Title	Metabolomics Analysis of Zebrafish Liver Under Long-Term BPF Exposure
Study Summary	In this project, we exposed both female and male adult zebrafish to blank, 1, 10 and 100 μg/L bisphenol F (BPF) for 7 weeks to study the influence of chronic BPF exposure on adult zebrafish liver. After experiments, fish were euthanized and metabolites were extracted from zebrafish liver for untargeted metabolomics analysis. This project aims to provide an integrative picture of sex-dependent metabolic alterations induced by chronic BPF exposure on adult zebrafish.
Institute	Washington University in St. Louis
Last Name	Guo
First Name	Qiuyuan
Address	1 Brookings Dr, St. Louis, MO 63130
Email	qiuyuan.g@wustl.edu
Phone	314-229-3635
Submit Date	2025-03-18
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2025-08-15
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR002378
Project DOI:	doi: 10.21228/M87Z5M
Project Title:	Metabolomics Analysis of Zebrafish Liver Under Long-Term BPF Exposure
Project Summary:	In this project, we exposed both female and male adult zebrafish to blank, 1, 10 and 100 μg/L bisphenol F (BPF) for 7 weeks to study the influence of chronic BPF exposure on adult zebrafish liver. After experiments, fish were euthanized and metabolites were extracted from zebrafish liver for untargeted metabolomics analysis. This project aims to provide an integrative picture of sex-dependent metabolic alterations induced by chronic BPF exposure on adult zebrafish.
Institute:	Washington University in St. Louis
Department:	Chemistry
Laboratory:	Patti Lab
Last Name:	Guo
First Name:	Qiuyuan
Address:	1 Brookings Dr, St. Louis, Missouri, 63130, USA
Email:	qiuyuan.g@wustl.edu
Phone:	314-229-3635

Subject:

Subject ID:	SU003938
Subject Type:	Fish
Subject Species:	Danio rerio
Taxonomy ID:	7955
Genotype Strain:	SjA
Age Or Age Range:	10 month old
Gender:	Male and female

Factors:

Subject type: Fish; Subject species: Danio rerio (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment
SA416595	100ugM02	100 ug/L BPF
SA416596	100ugM03	100 ug/L BPF
SA416597	100ugF08	100 ug/L BPF
SA416598	100ugF07	100 ug/L BPF
SA416599	100ugF06	100 ug/L BPF
SA416600	100ugF05	100 ug/L BPF
SA416601	100ugF04	100 ug/L BPF
SA416602	100ugF03	100 ug/L BPF
SA416603	100ugF02	100 ug/L BPF
SA416604	100ugF01	100 ug/L BPF
SA416605	100ugM08	100 ug/L BPF
SA416606	100ugM07	100 ug/L BPF
SA416607	100ugM06	100 ug/L BPF
SA416608	100ugM05	100 ug/L BPF
SA416609	100ugM04	100 ug/L BPF
SA416610	100ugM01	100 ug/L BPF
SA416580	10ugM03	10 ug/L BPF
SA416581	10ugM04	10 ug/L BPF
SA416582	10ugM05	10 ug/L BPF
SA416583	10ugM06	10 ug/L BPF
SA416584	10ugM07	10 ug/L BPF
SA416585	10ugF01	10 ug/L BPF
SA416586	10ugF02	10 ug/L BPF
SA416587	10ugF03	10 ug/L BPF
SA416588	10ugF04	10 ug/L BPF
SA416589	10ugF05	10 ug/L BPF
SA416590	10ugF06	10 ug/L BPF
SA416591	10ugF07	10 ug/L BPF
SA416592	10ugF08	10 ug/L BPF
SA416593	10ugM02	10 ug/L BPF
SA416594	10ugM01	10 ug/L BPF
SA416565	1ugM01	1 ug/L BPF
SA416566	1ugF06	1 ug/L BPF
SA416567	1ugF05	1 ug/L BPF
SA416568	1ugF04	1 ug/L BPF
SA416569	1ugF03	1 ug/L BPF
SA416570	1ugF02	1 ug/L BPF
SA416571	1ugF01	1 ug/L BPF
SA416572	1ugM07	1 ug/L BPF
SA416573	1ugM06	1 ug/L BPF
SA416574	1ugM05	1 ug/L BPF
SA416575	1ugM04	1 ug/L BPF
SA416576	1ugM03	1 ug/L BPF
SA416577	1ugM02	1 ug/L BPF
SA416578	1ugF07	1 ug/L BPF
SA416579	1ugF08	1 ug/L BPF
SA416611	blankM02	Blank
SA416612	blankF08	Blank
SA416613	blankM03	Blank
SA416614	blankM04	Blank
SA416615	blankM05	Blank
SA416616	blankM06	Blank
SA416617	blankM07	Blank
SA416618	blankM08	Blank
SA416619	blankF01	Blank
SA416620	blankF02	Blank
SA416621	blankF03	Blank
SA416622	blankF04	Blank
SA416623	blankF05	Blank
SA416624	blankF06	Blank
SA416625	blankF07	Blank
SA416626	blankM01	Blank

Showing results 1 to 62 of 62

Showing results 1 to 62 of 62

Collection:

Collection ID:	CO003931
Collection Summary:	After 7 weeks of exposure, fish were euthanized using gradual cooling. Liver from zebrafish were harvested in pre-weighed 1.5 mL Eppendorf tube, snap frozen in liquid nitrogen and stored in -80℃ until metabolite extraction.
Sample Type:	Liver

Treatment:

Treatment ID:	TR003947
Treatment Summary:	Stock solutions of bisphenol F (BPF, 10 mg/L, 100 mg/L and 1000 mg/L) were prepared in DMSO and stored in the dark at 4℃. Experiments were performed using adult (10-month old) zebrafish. Eight female and eight male fish were exposed to 1 μg/L, 10 μg/L and 100 μg/L BPF and a blank control (DMSO 0.01% v/v) in 5 L tanks for 7 weeks, respectively. Importantly, the lowest concentration used (1 ug/L) was corresponding to the detected BPF levels in surface water samples. During the experiment, all tanks were maintained under the same pre-experimental conditions as described above, in which fish were allowed to swim freely. Fish were fed with Gemma micro 300 zebrafish food (Skretting) daily at noon (4 g/per fish) and the water in all tanks was changed every other day. After 7 weeks of exposure, fish were euthanized using gradual cooling. Liver from zebrafish were harvested in pre-weighed 1.5 mL Eppendorf tube, snap frozen in liquid nitrogen and stored in -80℃ until metabolite extraction. Metabolic extraction from zebrafish liver for LC/MS analysis

Treatment ID:

TR003947

Treatment Summary:

Stock solutions of bisphenol F (BPF, 10 mg/L, 100 mg/L and 1000 mg/L) were prepared in DMSO and stored in the dark at 4℃. Experiments were performed using adult (10-month old) zebrafish. Eight female and eight male fish were exposed to 1 μg/L, 10 μg/L and 100 μg/L BPF and a blank control (DMSO 0.01% v/v) in 5 L tanks for 7 weeks, respectively. Importantly, the lowest concentration used (1 ug/L) was corresponding to the detected BPF levels in surface water samples. During the experiment, all tanks were maintained under the same pre-experimental conditions as described above, in which fish were allowed to swim freely. Fish were fed with Gemma micro 300 zebrafish food (Skretting) daily at noon (4 g/per fish) and the water in all tanks was changed every other day. After 7 weeks of exposure, fish were euthanized using gradual cooling. Liver from zebrafish were harvested in pre-weighed 1.5 mL Eppendorf tube, snap frozen in liquid nitrogen and stored in -80℃ until metabolite extraction. Metabolic extraction from zebrafish liver for LC/MS analysis

Sample Preparation:

Sampleprep ID:	SP003944
Sampleprep Summary:	Frozen zebrafish liver was ground in the pre-weighed 1.5 ml Eppendorf tube with a disposable pestle prechilled in liquid nitrogen. The tube containing zebrafish liver was weighed again after grinding to calculate the net liver weight. Cold extraction buffer (2:2:1 ACN:MeOH:water with 0.1 M formic acid, 4℃) was added to the ground liver. For every 1 mg of liver wet weight, 40 μL of extraction solvent were added. The extraction mixture was then vortexed for 30 s and incubated on ice for 4 min. 2 M ammonium bicarbonate was added to the extraction buffer to adjust the pH to 8.5. The mixture was subjected to three cycles of freezing in liquid nitrogen (1 min), thawing in 25℃ water (10 s), sonication (5 min), and vortexing (30 s), followed by incubation at -20℃ for 1 h. After protein precipitation, liver extracts were centrifuged at 14000 g at 4℃ for 15 min. The supernatant was transferred to LC/MS vial for same-day analysis.

Sampleprep ID:

SP003944

Sampleprep Summary:

Frozen zebrafish liver was ground in the pre-weighed 1.5 ml Eppendorf tube with a disposable pestle prechilled in liquid nitrogen. The tube containing zebrafish liver was weighed again after grinding to calculate the net liver weight. Cold extraction buffer (2:2:1 ACN:MeOH:water with 0.1 M formic acid, 4℃) was added to the ground liver. For every 1 mg of liver wet weight, 40 μL of extraction solvent were added. The extraction mixture was then vortexed for 30 s and incubated on ice for 4 min. 2 M ammonium bicarbonate was added to the extraction buffer to adjust the pH to 8.5. The mixture was subjected to three cycles of freezing in liquid nitrogen (1 min), thawing in 25℃ water (10 s), sonication (5 min), and vortexing (30 s), followed by incubation at -20℃ for 1 h. After protein precipitation, liver extracts were centrifuged at 14000 g at 4℃ for 15 min. The supernatant was transferred to LC/MS vial for same-day analysis.

Chromatography:

Chromatography ID:	CH004742
Instrument Name:	Agilent 1260
Column Name:	HILICON iHILIC-(P) Classic (100 x 2.1 mm, 5 μm, 200A)
Column Temperature:	40℃
Flow Gradient:	0.25 mL/min: 0-1 min: 90%B, 1-12 min: 35%B, 12-12.5 min: 25%B, 12.5-14.5 min: 25%B, 14.5-15 min: 90%B, 15-15.5 min: 90%B, 0.40 mL/min: 15.5-16.5 min: 90%B, 16.5-20 min:90%B, 0.25 mL/min: 20-20.5 min: 90%B, 20.5-24 min: 90%B,
Flow Rate:	0.25 mL/min
Solvent A:	95% Water/5% Acetonitrile; 20 mM Ammonium bicarbonate; 0.1% Ammonium hydroxide; 5 uM Medronic acid
Solvent B:	95% Acetonitrile/5% Water
Chromatography Type:	HILIC

Analysis:

Analysis ID:	AN006253
Analysis Type:	MS
Chromatography ID:	CH004742
Num Factors:	4
Num Metabolites:	117
Units:	peak area