Summary of Study ST004236
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002673. The data can be accessed directly via it's Project DOI: 10.21228/M84P1D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004236 |
| Study Title | Lipidomics analysis of TERT-hWA adipocytes spheroids under basal and stimulated lipolysis conditions |
| Study Summary | TERT-hWA spheroids (5 per replicate) were treated with DMSO or 1 µM FK for 3 h, after which spheroids were collected and subjected to lipid extraction for lipidomic analysis. LC–MS/MS was then used to quantify individual triacylglycerol (TG) species under basal and stimulated lipolysis conditions, allowing us to assess their susceptibility to lipolysis. The results reveal that TGs containing short- and medium-chain fatty acids (MSCFA-TGs) are markedly more prone to lipolysis than conventional TG species. |
| Institute | University of Szeged |
| Last Name | David |
| First Name | Kovacs |
| Address | Dóm tér 9, 6723 |
| kovacs.david@med.u-szeged.hu | |
| Phone | +3662342665 |
| Submit Date | 2025-09-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-10-20 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002673 |
| Project DOI: | doi: 10.21228/M84P1D |
| Project Title: | Chain length-dependent mobilization and oxidation of fatty acids in adipocytes |
| Project Summary: | Fatty acids (FAs) are essential metabolites in energy homeostasis. Adipocytes store FAs as triacylglycerol (TG) in their lipid droplets and mobilize them upon demand in order to provide energy for peripheral tissues. While the mobilization of long-chain fatty acids (LCFAs) during lipolysis is largely documented, there is little information on the metabolism of short- and medium-chain fatty acids (SMCFA) in adipocytes. We demonstrate that adipocytes store SMCFAs in their lipid droplets and that following lipolysis initiation, TGs containing SMCFAs undergo rapid hydrolysis. We found that this process is facilitated by the preferential accumulation of SMCFA-containing TGs at the surface of lipid droplets, thereby enhancing their accessibility to lipases. Unlike LCFAs, SMCFAs are not released from the adipocytes following lipolysis but undergo oxidation within the cell. Our findings suggest that SMCFAs are preferentially mobilized and oxidized to provide energy for the adipocyte, highlighting a distinct metabolic fate compared to LCFAs. |
| Institute: | University of Szeged |
| Department: | Institute of Biochemistry |
| Last Name: | Kovacs |
| First Name: | David |
| Address: | Dóm tér 9, Szeged, Csongrád-Csanád, 6723, Hungary |
| Email: | kovacs.david@med.u-szeged.hu |
| Phone: | +3662342665 |
Subject:
| Subject ID: | SU004388 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Condition | Injection order | Sample source |
|---|---|---|---|---|
| SA486420 | 1 | Blank | 1 | Adipocyte_spheroids |
| SA486421 | 31 | Blank | 1 | Adipocyte_spheroids |
| SA486422 | 2 | Blank | 2 | Adipocyte_spheroids |
| SA486423 | 32 | Blank | 2 | Adipocyte_spheroids |
| SA486424 | 3 | Blank | 3 | Adipocyte_spheroids |
| SA486425 | 33 | Blank | 3 | Adipocyte_spheroids |
| SA486426 | 4 | C | 1 | Adipocyte_spheroids |
| SA486427 | 42 | C | 1 | Adipocyte_spheroids |
| SA486428 | 38 | C | 1 | Adipocyte_spheroids |
| SA486429 | 34 | C | 1 | Adipocyte_spheroids |
| SA486430 | 12 | C | 1 | Adipocyte_spheroids |
| SA486431 | 8 | C | 1 | Adipocyte_spheroids |
| SA486432 | 5 | C | 2 | Adipocyte_spheroids |
| SA486433 | 43 | C | 2 | Adipocyte_spheroids |
| SA486434 | 39 | C | 2 | Adipocyte_spheroids |
| SA486435 | 9 | C | 2 | Adipocyte_spheroids |
| SA486436 | 13 | C | 2 | Adipocyte_spheroids |
| SA486437 | 35 | C | 2 | Adipocyte_spheroids |
| SA486438 | 44 | C | 3 | Adipocyte_spheroids |
| SA486439 | 40 | C | 3 | Adipocyte_spheroids |
| SA486440 | 36 | C | 3 | Adipocyte_spheroids |
| SA486441 | 10 | C | 3 | Adipocyte_spheroids |
| SA486442 | 6 | C | 3 | Adipocyte_spheroids |
| SA486443 | 14 | C | 3 | Adipocyte_spheroids |
| SA486444 | 37 | C | 4 | Adipocyte_spheroids |
| SA486445 | 11 | C | 4 | Adipocyte_spheroids |
| SA486446 | 45 | C | 4 | Adipocyte_spheroids |
| SA486447 | 41 | C | 4 | Adipocyte_spheroids |
| SA486448 | 7 | C | 4 | Adipocyte_spheroids |
| SA486449 | 15 | C | 4 | Adipocyte_spheroids |
| SA486450 | 24 | Lipolysis | 1 | Adipocyte_spheroids |
| SA486451 | 16 | Lipolysis | 1 | Adipocyte_spheroids |
| SA486452 | 54 | Lipolysis | 1 | Adipocyte_spheroids |
| SA486453 | 20 | Lipolysis | 1 | Adipocyte_spheroids |
| SA486454 | 50 | Lipolysis | 1 | Adipocyte_spheroids |
| SA486455 | 46 | Lipolysis | 1 | Adipocyte_spheroids |
| SA486456 | 55 | Lipolysis | 2 | Adipocyte_spheroids |
| SA486457 | 51 | Lipolysis | 2 | Adipocyte_spheroids |
| SA486458 | 47 | Lipolysis | 2 | Adipocyte_spheroids |
| SA486459 | 25 | Lipolysis | 2 | Adipocyte_spheroids |
| SA486460 | 17 | Lipolysis | 2 | Adipocyte_spheroids |
| SA486461 | 21 | Lipolysis | 2 | Adipocyte_spheroids |
| SA486462 | 18 | Lipolysis | 3 | Adipocyte_spheroids |
| SA486463 | 48 | Lipolysis | 3 | Adipocyte_spheroids |
| SA486464 | 26 | Lipolysis | 3 | Adipocyte_spheroids |
| SA486465 | 52 | Lipolysis | 3 | Adipocyte_spheroids |
| SA486466 | 22 | Lipolysis | 3 | Adipocyte_spheroids |
| SA486467 | 56 | Lipolysis | 3 | Adipocyte_spheroids |
| SA486468 | 53 | Lipolysis | 4 | Adipocyte_spheroids |
| SA486469 | 57 | Lipolysis | 4 | Adipocyte_spheroids |
| SA486470 | 23 | Lipolysis | 4 | Adipocyte_spheroids |
| SA486471 | 49 | Lipolysis | 4 | Adipocyte_spheroids |
| SA486472 | 19 | Lipolysis | 4 | Adipocyte_spheroids |
| SA486473 | 27 | Lipolysis | 4 | Adipocyte_spheroids |
| SA486474 | 58 | Standards | 1 | Adipocyte_spheroids |
| SA486475 | 28 | Standards | 1 | Adipocyte_spheroids |
| SA486476 | 59 | Standards | 2 | Adipocyte_spheroids |
| SA486477 | 29 | Standards | 2 | Adipocyte_spheroids |
| SA486478 | 30 | Standards | 3 | Adipocyte_spheroids |
| SA486479 | 60 | Standards | 3 | Adipocyte_spheroids |
| Showing results 1 to 60 of 60 |
Collection:
| Collection ID: | CO004381 |
| Collection Summary: | Spheroids were pooled in plastic test tubes (5 spheroid/replicate) and lipolysis was induced by Forskolin addition. Following a 3 h incubation at 37 degree, spheroids were collected in a minimal volume of PBS, then ACN and Hex phases were extracted from TERT-hWA as described in the Sampleprep section. The phases were analysed by LC-MS/MS and each sample was injected at least times. |
| Sample Type: | Adipocyte spheroids |
Treatment:
| Treatment ID: | TR004397 |
| Treatment Summary: | Pooled spheroids (5/sample) were treated with DMSO (C) or 1 uM Forskolin (Lipolysis) for 3 hours |
Sample Preparation:
| Sampleprep ID: | SP004394 |
| Sampleprep Summary: | To extract lipids from spheroids, a 3-phase liquid extraction was performed to separate neutral and polar lipids. Adipocytes were collected in a minimum phosphate-buffered saline (PBS) volume (aqueous). Spheroids were transferred into glass tubes, containing already the internal standard mix (Splash) and TG 6:0/6:0/6:0. Then, to reduce sample loss, 0.75 mL of ACN were added to microtubes, vortexed, and transferred into the corresponding glass tubes. The remaining solvents were added to each sample: 0.75 mL Hex, 0.25 mL EtAc. The aqueous phase (sample) was completed with ultrapure water when needed, resulting in Hex:EtAc:ACN:Aqueous (3:1:3:2, V:V:V:V). Spheroid samples were first vortexed for 30 min at 4oC with glass beads, and only then centrifuged. The upper phase was collected into a new tube with a Hamilton glass syringe, which was washed three times in solvent between samples. Hex was added (half the volume of the first extraction) to the 2 remaining phases for re-extraction. Samples were again vortexed and centrifuged, and upper and middle phases were collected separately. To reduce phospholipid loss, a re-extraction of middle phase was done with ACN:EtAc (3:1, V:V) (half the volume of first extraction). Extraction (solvents and water) and experiment blanks (PBS) were included. All extraction solvents had 50 μg/mL BHT. Extracted samples were kept dried at -20oC under Ar. |
Chromatography:
| Chromatography ID: | CH005355 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Accucore C18 (150 x 2.1mm,2.6um) |
| Column Temperature: | 35°C |
| Flow Gradient: | 0.0 min, 35% B; 4.0 min, 60% B; 8.0 min, 70% B; 16.0 min, 85% B; 25.0 min, 97% B |
| Flow Rate: | 400 µl/min |
| Solvent A: | 50% Acetonitrile/50% Water; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B: | 88% Isopropanom/10% Acetonitrile/2% Water; 2 mM ammonium formate; 0.02 % formic acid |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN007053 |
| Laboratory Name: | CNRS Institute of Molecular and Cellular Pharmacology |
| Analysis Type: | MS |
| Chromatography ID: | CH005355 |
| Num Factors: | 14 |
| Num Metabolites: | 160 |
| Units: | Peak Intensity |
