Summary of Study ST004237
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002673. The data can be accessed directly via it's Project DOI: 10.21228/M84P1D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004237 |
| Study Title | Lipidomic analysis of the perigonadal adipose tissue of Control, HSL KO and ATGL KO mice. |
| Study Summary | AdipoCreERT2⁺ and AdipoCreERT2⁻ (control) mice carrying floxed alleles of Lipe (HSL) or Pnpla2 (ATGL) were treated with 4-hydroxy-tamoxifen to induce adipocyte-specific depletion of HSL or ATGL. Following gene deletion, white adipose tissue (WAT) samples were collected, and lipids were extracted. The hexane phases were isolated and analyzed by LC–MS/MS to quantify the complete triacylglycerol (TG) profile. While all TG species were measured, we specifically focused on short- and medium-chain fatty acid–containing TGs (SMCFA-TGs), where we detected marked differences in abundance between genotypes depending on the presence or absence of HSL or ATGL. |
| Institute | University of Szeged |
| Last Name | David |
| First Name | Kovacs |
| Address | Dóm tér 9, 6723 |
| kovacs.david@med.u-szeged.hu | |
| Phone | +3662342665 |
| Submit Date | 2025-09-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-10-20 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002673 |
| Project DOI: | doi: 10.21228/M84P1D |
| Project Title: | Chain length-dependent mobilization and oxidation of fatty acids in adipocytes |
| Project Summary: | Fatty acids (FAs) are essential metabolites in energy homeostasis. Adipocytes store FAs as triacylglycerol (TG) in their lipid droplets and mobilize them upon demand in order to provide energy for peripheral tissues. While the mobilization of long-chain fatty acids (LCFAs) during lipolysis is largely documented, there is little information on the metabolism of short- and medium-chain fatty acids (SMCFA) in adipocytes. We demonstrate that adipocytes store SMCFAs in their lipid droplets and that following lipolysis initiation, TGs containing SMCFAs undergo rapid hydrolysis. We found that this process is facilitated by the preferential accumulation of SMCFA-containing TGs at the surface of lipid droplets, thereby enhancing their accessibility to lipases. Unlike LCFAs, SMCFAs are not released from the adipocytes following lipolysis but undergo oxidation within the cell. Our findings suggest that SMCFAs are preferentially mobilized and oxidized to provide energy for the adipocyte, highlighting a distinct metabolic fate compared to LCFAs. |
| Institute: | University of Szeged |
| Department: | Institute of Biochemistry |
| Last Name: | Kovacs |
| First Name: | David |
| Address: | Dóm tér 9, Szeged, Csongrád-Csanád, 6723, Hungary |
| Email: | kovacs.david@med.u-szeged.hu |
| Phone: | +3662342665 |
Subject:
| Subject ID: | SU004389 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype | Injection order | Sample source |
|---|---|---|---|---|
| SA486480 | 221130_C-806_Pos1 | ATGL KO | 1 | WAT |
| SA486481 | 221130_C-807_Pos1 | ATGL KO | 1 | WAT |
| SA486482 | 221130_C-841_Pos1 | ATGL KO | 1 | WAT |
| SA486483 | 221130_C-843_Pos1 | ATGL KO | 1 | WAT |
| SA486484 | 221130_C-807_Pos2 | ATGL KO | 2 | WAT |
| SA486485 | 221130_C-841_Pos2 | ATGL KO | 2 | WAT |
| SA486486 | 221130_C-843_Pos2 | ATGL KO | 2 | WAT |
| SA486487 | 221130_C-806_Pos2 | ATGL KO | 2 | WAT |
| SA486488 | 221130_C-841_Pos3 | ATGL KO | 3 | WAT |
| SA486489 | 221130_C-807_Pos3 | ATGL KO | 3 | WAT |
| SA486490 | 221130_C-843_Pos3 | ATGL KO | 3 | WAT |
| SA486491 | 221130_C-806_Pos3 | ATGL KO | 3 | WAT |
| SA486492 | 221130_C-841_Pos4 | ATGL KO | 4 | WAT |
| SA486493 | 221130_C-807_Pos4 | ATGL KO | 4 | WAT |
| SA486494 | 221130_C-843_Pos4 | ATGL KO | 4 | WAT |
| SA486495 | 221130_C-806_Pos4 | ATGL KO | 4 | WAT |
| SA486496 | 221130_A-808_Pos1 | Control | 1 | WAT |
| SA486497 | 221130_A-824_Pos1 | Control | 1 | WAT |
| SA486498 | 221130_A-826_Pos1 | Control | 1 | WAT |
| SA486499 | 221130_A-834_Pos1 | Control | 1 | WAT |
| SA486500 | 221130_A-824_Pos2 | Control | 2 | WAT |
| SA486501 | 221130_A-808_Pos2 | Control | 2 | WAT |
| SA486502 | 221130_A-826_Pos2 | Control | 2 | WAT |
| SA486503 | 221130_A-834_Pos2 | Control | 2 | WAT |
| SA486504 | 221130_A-808_Pos3 | Control | 3 | WAT |
| SA486505 | 221130_A-824_Pos3 | Control | 3 | WAT |
| SA486506 | 221130_A-826_Pos3 | Control | 3 | WAT |
| SA486507 | 221130_A-834_Pos3 | Control | 3 | WAT |
| SA486508 | 221130_A-834_Pos4 | Control | 4 | WAT |
| SA486509 | 221130_A-808_Pos4 | Control | 4 | WAT |
| SA486510 | 221130_A-824_Pos4 | Control | 4 | WAT |
| SA486511 | 221130_A-826_Pos4 | Control | 4 | WAT |
| SA486512 | 221130_B-781_Pos1 | HSL KO | 1 | WAT |
| SA486513 | 221130_B-778_Pos1 | HSL KO | 1 | WAT |
| SA486514 | 221130_B-780_Pos1 | HSL KO | 1 | WAT |
| SA486515 | 221130_B-835_Pos1 | HSL KO | 1 | WAT |
| SA486516 | 221130_B-778_Pos2 | HSL KO | 2 | WAT |
| SA486517 | 221130_B-835_Pos2 | HSL KO | 2 | WAT |
| SA486518 | 221130_B-781_Pos2 | HSL KO | 2 | WAT |
| SA486519 | 221130_B-780_Pos2 | HSL KO | 2 | WAT |
| SA486520 | 221130_B-835_Pos3 | HSL KO | 3 | WAT |
| SA486521 | 221130_B-781_Pos3 | HSL KO | 3 | WAT |
| SA486522 | 221130_B-780_Pos3 | HSL KO | 3 | WAT |
| SA486523 | 221130_B-778_Pos3 | HSL KO | 3 | WAT |
| SA486524 | 221130_B-778_Pos4 | HSL KO | 4 | WAT |
| SA486525 | 221130_B-835_Pos4 | HSL KO | 4 | WAT |
| SA486526 | 221130_B-781_Pos4 | HSL KO | 4 | WAT |
| SA486527 | 221130_B-780_Pos4 | HSL KO | 4 | WAT |
| Showing results 1 to 48 of 48 |
Collection:
| Collection ID: | CO004382 |
| Collection Summary: | The mouse models used in this study were generated previously by insertion of LoxP sites in the Lipe gene encoding HSL, or in the Pnpla2 gene encoding ATGL. To obtain adipocyte specific knock-out, these mice were crossed with transgenic mice (Tg(Adipoq-cre/ERT2)1Soff/J) expressing the tamoxifen-inducible Cre-ERT2 recombinase, expressed under the control of the adiponectin promoter, specific for adipose tissue. 7-week old mice were gavaged with tamoxifen (1 mg/day for 5 days, SIGMA T5648-1G) to induce adipocyte-specific deletion of HSL (HSLAdipo-/-) or ATGL (ATGLAdipo-/- mice). Control littermate were Atgl+/+ Hslfl/fl AdipoQ-Cre/ERT2-, Atglfl/fl Hsl+/+ AdipoQ-Cre/ERT2- and Hslfl/fl Atglfl/fl AdipoQ-Cre/ERT2-. Mice were fed chow (Ssniff V1534). Mice were housed and manipulated according to Inserm guidelines and European Directive 2010/63/UE in the local animal care facility (agreements A 31 555 04 and C 31 555 07). Protocols were approved by the French Ministry of Research after review by local ethical committee (comité d’éthique en experimentation animale de l’UMS006/CREFRE, CEEA122, Toulouse, France). For lipidomic analysis, perigonadal white adipose tissued were collected, and 8-13 mg samples were extracted. |
| Sample Type: | Adipose tissue |
Treatment:
| Treatment ID: | TR004398 |
| Treatment Summary: | Mice with different genotypes were used |
Sample Preparation:
| Sampleprep ID: | SP004395 |
| Sampleprep Summary: | To extract lipids from tissue samples, a 3-phase liquid extraction was performed to separate neutral and polar lipids. First, tissue samples were measured on an analytical scale then transferred into glass tubes, containing already the internal standard mix (Splash) and TG 6:0/6:0/6:0. Then, to reduce sample loss, 0.75 mL of ACN were added to microtubes, vortexed, and transferred into the corresponding glass tubes. The remaining solvents were added to each sample: 0.75 mL Hex, 0.25 mL EtAc. The aqueous phase (sample) was completed with ultrapure water when needed, resulting in Hex:EtAc:ACN:Aqueous (3:1:3:2, V:V:V:V). Tissue samples were first vortexed for 30 min at 4oC with glass beads, and only then centrifuged. The upper phase was collected into a new tube with a Hamilton glass syringe, which was washed three times in solvent between samples. Hex was added (half the volume of the first extraction) to the 2 remaining phases for re-extraction. Samples were again vortexed and centrifuged, and upper and middle phases were collected separately. To reduce phospholipid loss, a re-extraction of middle phase was done with ACN:EtAc (3:1, V:V) (half the volume of first extraction). Extraction (solvents and water) and experiment blanks (PBS) were included. All extraction solvents had 50 μg/mL BHT. Extracted samples were kept dried at -20oC under Ar. |
Chromatography:
| Chromatography ID: | CH005356 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Accucore C18 (150 x 2.1mm,2.6um) |
| Column Temperature: | 35°C |
| Flow Gradient: | 0.0 min, 35% B; 4.0 min, 60% B; 8.0 min, 70% B; 16.0 min, 85% B; 25.0 min, 97% B. |
| Flow Rate: | 400 µl/min |
| Solvent A: | 50% Acetonitrile/50% Water; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B: | 88% Isopropanol/10% Acetonitrile/2% Water; 2 mM ammonium formate; 0.02 % formic acid |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN007054 |
| Laboratory Name: | CNRS Institute of Molecular and Cellular Pharmacology |
| Analysis Type: | MS |
| Chromatography ID: | CH005356 |
| Num Factors: | 12 |
| Num Metabolites: | 184 |
| Units: | Peak Intensity |
