Summary of Study ST004376
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002774. The data can be accessed directly via it's Project DOI: 10.21228/M8383Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004376 |
| Study Title | Convolvulus pluricaulis confers antidepressant and antioxidant effects through conserved metabolic and molecular pathways in Drosophila |
| Study Summary | Convolvulus pluricaulis (Shankhpushpi) is a traditional herb used to treat depression and anxiety. Using Drosophila melanogaster as a model, we identified conserved metabolic and molecular pathways mediating its neuroprotective effects. Metabolomic profiling of flies fed C. pluricaulis revealed altered levels of ascorbic acid, glucose, and adenine monophosphate in head tissue. Gene expression analysis showed significant modulation of glut1 (glucose transporter 1), CG6293 (ascorbate transporter), Rdl (resistant to dieldrin), GABA-B-R1 (GABA-B receptor 1), and sod1 (superoxide dismutase 1). Dietary C. pluricaulis reduced depression-like behavior in a stress-induced model and elevated head ascorbate levels. Knockdown of CG6293, sod1, glut1, or GABA-B-R1 abolished the antioxidant effects, and knockdown of CG6293 eliminated the antidepressant effects, implicating these genes as key downstream effectors. Supplementation with L-ascorbic acid mimicked the behavioral and oxidative resilience conferred by C. pluricaulis. Together, these findings reveal conserved antioxidant and anxiolytic mechanisms underlying the pharmacological effects of C. pluricaulis in Drosophila. |
| Institute | Shiv Nadar Institution of Eminence, Greater Noida, India |
| Department | Department of Life Sciences |
| Laboratory | RNA Biology Laboratory |
| Last Name | Chawla |
| First Name | Geetanjali |
| Address | NH91, Tehsil Dadri, Greater Noida, U.P-201314, INDIA |
| geetanjalichawla25@gmail.com | |
| Phone | +91-9205007117 |
| Submit Date | 2025-11-19 |
| Num Groups | 2 |
| Total Subjects | 200 |
| Num Females | 200 |
| Publications | Mitra et al., Convolvulus pluricaulis confers antidepressant and antioxidant effects through conserved metabolic and molecular pathways in Drosophila |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2025-11-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002774 |
| Project DOI: | doi: 10.21228/M8383Q |
| Project Title: | Convolvulus pluricaulis confers antidepressant and antioxidant effects through conserved metabolic pathways in Drosophila |
| Project Type: | GC-MS |
| Project Summary: | Convolvulus pluricaulis (Shankhpushpi) is a traditional herb used to treat depression and anxiety. Using Drosophila melanogaster as a model, we identified conserved metabolic and molecular pathways mediating its neuroprotective effects. Metabolomic profiling of flies fed C. pluricaulis revealed altered levels of ascorbic acid, glucose, and adenine monophosphate in head tissue. Gene expression analysis showed significant modulation of glut1 (glucose transporter 1), CG6293 (ascorbate transporter), Rdl (resistant to dieldrin), GABA-B-R1 (GABA-B receptor 1), and sod1 (superoxide dismutase 1). Dietary C. pluricaulis reduced depression-like behavior in a stress-induced model and elevated head ascorbate levels. Knockdown of CG6293, sod1, glut1, or GABA-B-R1 abolished the antioxidant effects, and knockdown of CG6293 eliminated the antidepressant effects, implicating these genes as key downstream effectors. Supplementation with L-ascorbic acid mimicked the behavioral and oxidative resilience conferred by C. pluricaulis. Together, these findings reveal conserved antioxidant and anxiolytic mechanisms underlying the pharmacological effects of C. pluricaulis in Drosophila. |
| Institute: | Shiv Nadar Institution of Eminence, Greater Noida, India |
| Department: | Department of Life Sciences |
| Laboratory: | RNA Biology Laboratory |
| Last Name: | Chawla |
| First Name: | Geetanjali |
| Address: | NH91, Tehsil Dadri, Greater Noida, U.P-201314, INDIA |
| Email: | geetanjalichawla25@gmail.com |
| Phone: | +91-9205007117 |
| Funding Source: | DBT-Wellcome Trust India Alliance |
| Publications: | Mitra et al., Convolvulus pluricaulis confers antidepressant and antioxidant effects through conserved metabolic and molecular pathways in Drosophila |
| Contributors: | Geetanjali Chawla |
Subject:
| Subject ID: | SU004535 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Genotype Strain: | Canton S |
| Age Or Age Range: | 23-25 Days |
| Weight Or Weight Range: | 0.7-1mg |
| Height Or Height Range: | 1mm |
| Gender: | Female |
| Species Group: | Drosophila melanogaster |
Factors:
Subject type: Insect; Subject species: Drosophila melanogaster (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Treatment |
|---|---|---|---|
| SA520194 | Ctrl 20d-2B | Body tissue | Control |
| SA520195 | Ctrl 20d-4B | Body tissue | Control |
| SA520196 | Ctrl 20d-3B | Body tissue | Control |
| SA520197 | Ctrl 20d-1B | Body tissue | Control |
| SA520190 | C.p 20d-4B | Body tissue | C. pluricaulis |
| SA520191 | C.p 20d-3B | Body tissue | C. pluricaulis |
| SA520192 | C.p 20d-2B | Body tissue | C. pluricaulis |
| SA520193 | C.p 20d-1B | Body tissue | C. pluricaulis |
| SA520202 | Ctrl 20d-2H | Head tissue | Control |
| SA520203 | Ctrl 20d-4H | Head tissue | Control |
| SA520204 | Ctrl 20d-3H | Head tissue | Control |
| SA520205 | Ctrl 20d-1H | Head tissue | Control |
| SA520198 | C.p 20d-4H | Head tissue | C. pluricaulis |
| SA520199 | C.p 20d-3H | Head tissue | C. pluricaulis |
| SA520200 | C.p 20d-2H | Head tissue | C. pluricaulis |
| SA520201 | C.p 20d-1H | Head tissue | C. pluricaulis |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO004528 |
| Collection Summary: | Fly heads were dissected, counted and frozen in liquid nitrogen. The remaining body tissue was frozen in separate vials. The samples were frozen in liquid nitrogen in 2 mL screw cap vials with ceramic beads and stored in -80°C until preparation of the lysate. |
| Sample Type: | Head and Body tissue |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR004544 |
| Treatment Summary: | Canton S flies were fed a regular cornmeal diet (Vehicle) or a Convolvulus pluricaulis diet (treatment) for 20 Days. |
| Treatment Compound: | Convolvulus pluricaulis |
| Treatment Dose: | 100X |
| Treatment Vehicle: | Ethanol |
Sample Preparation:
| Sampleprep ID: | SP004541 |
| Sampleprep Summary: | The sample lysate was prepared by homogenizing the samples in 90% chilled methanol, followed by one hour incubation at -20°C to precipitate proteins, and the sample was centrifuged to remove insoluble debris. The supernatant was dried in a SpeedVac, and the metabolite containing precipitate was analysed. |
Chromatography:
| Chromatography ID: | CH005551 |
| Chromatography Summary: | All GC-MS analysis was performed with an Agilent 5977b GC-MS MSD-HES and an Agilent 7693A automatic liquid sampler. Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath. 25 µL of this solution was added to auto sampler vials. 60 µL of N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, Thermo #TS48913) was added automatically via the auto sampler and incubated for 30 minutes at 37 °C. After incubation, samples were vortexed and 1 µL of the prepared sample was injected into the gas chromatograph inlet in the split mode with the inlet temperature held at 250°C. A 50:1 split ratio was used for analysis for the majority of metabolites. Any metabolites that saturated the instrument at the 50:1 split were analyzed at a 150:1 split ratio. The gas chromatograph had an initial temperature of 60°C for one minute followed by a 10°C/min ramp to 325°C and a hold time of 10 minutes. A 30-meter Agilent Zorbax DB-5MS with 10 m Duraguard capillary column was employed for chromatographic separation. Helium was used as the carrier gas at a rate of 1 mL/min. |
| Instrument Name: | Agilent 5977b GC-MS |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Column Temperature: | 60°C for one minute followed by a 10°C/min ramp to 325°C and a hold time of 10 minutes |
| Flow Gradient: | NA |
| Flow Rate: | NA |
| Solvent A: | NA |
| Solvent B: | NA |
| Chromatography Type: | GC |
| Solvent C: | NA |
Analysis:
| Analysis ID: | AN007312 |
| Analysis Type: | MS |
| Acquisition Date: | 2020-02-19 |
| Chromatography ID: | CH005551 |
| Num Factors: | 4 |
| Num Metabolites: | 118 |
| Units: | Normalized and scaled peak intensity (arbitrary units, a.u.) |
